Zinc in PDB 7wnt: Rnase J From Mycobacterium Tuberculosis

All present enzymatic activity of Rnase J From Mycobacterium Tuberculosis:
3.5.2.6;

The structure of Rnase J From Mycobacterium Tuberculosis, PDB code: 7wnt was solved by J.Li, L.Bao, J.Hu, B.Zhan, Z.Li, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.64 / 2.44
Space group	P 64 2 2
Cell size a, b, c (Å), α, β, γ (°)	128.52, 128.52, 184.276, 90, 90, 120
R / Rfree (%)	21.9 / 25.1

The binding sites of Zinc atom in the Rnase J From Mycobacterium Tuberculosis (pdb code 7wnt). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Rnase J From Mycobacterium Tuberculosis, PDB code: 7wnt:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Rnase J From Mycobacterium Tuberculosis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rnase J From Mycobacterium Tuberculosis within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:62.0 occ:1.00 OD2 A:ASP170 2.3 54.0 1.0 ND1 A:HIS83 2.4 50.3 1.0 NE2 A:HIS81 2.4 39.4 1.0 NE2 A:HIS148 2.5 46.6 1.0 O A:HOH776 3.0 49.1 1.0 ZN A:ZN602 3.2 59.5 1.0 CE1 A:HIS83 3.3 45.8 1.0 CE1 A:HIS81 3.3 41.6 1.0 CE1 A:HIS148 3.3 44.1 1.0 CG A:ASP170 3.4 46.3 1.0 CD2 A:HIS81 3.4 43.9 1.0 CG A:HIS83 3.4 43.2 1.0 CD2 A:HIS148 3.6 43.7 1.0 CB A:HIS83 3.7 39.5 1.0 CB A:ASP170 3.8 44.5 1.0 NE2 A:HIS86 4.0 50.8 1.0 CD2 A:HIS86 4.2 50.0 1.0 NE2 A:HIS83 4.4 52.2 1.0 CE1 A:HIS375 4.4 50.1 1.0 OD1 A:ASP85 4.4 51.0 1.0 OD1 A:ASP170 4.4 54.0 1.0 ND1 A:HIS81 4.5 39.9 1.0 CD2 A:HIS83 4.5 46.7 1.0 CG A:HIS81 4.5 38.5 1.0 NE2 A:HIS375 4.5 51.7 1.0 ND1 A:HIS148 4.5 50.6 1.0 O A:HOH738 4.6 51.4 1.0 CG A:HIS148 4.7 51.3 1.0 NE2 A:HIS397 5.0 55.6 1.0

Zinc binding site 2 out of 2 in the Rnase J From Mycobacterium Tuberculosis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rnase J From Mycobacterium Tuberculosis within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn602 b:59.5 occ:1.00 NE2 A:HIS397 2.0 55.6 1.0 OD2 A:ASP170 2.2 54.0 1.0 NE2 A:HIS86 2.3 50.8 1.0 OD2 A:ASP85 2.4 55.8 1.0 CE1 A:HIS397 2.7 55.0 1.0 O A:HOH776 2.8 49.1 1.0 CD2 A:HIS86 3.1 50.0 1.0 CG A:ASP170 3.1 46.3 1.0 CD2 A:HIS397 3.1 55.2 1.0 ZN A:ZN601 3.2 62.0 1.0 CG A:ASP85 3.2 52.2 1.0 OD1 A:ASP85 3.3 51.0 1.0 OD1 A:ASP170 3.4 54.0 1.0 CE1 A:HIS86 3.4 49.6 1.0 ND1 A:HIS397 3.9 51.1 1.0 CG A:HIS397 4.2 52.9 1.0 ND2 A:ASN30 4.3 52.1 1.0 CE1 A:HIS375 4.3 50.1 1.0 CG A:HIS86 4.3 50.1 1.0 NE2 A:HIS81 4.4 39.4 1.0 CE1 A:HIS81 4.4 41.6 1.0 ND1 A:HIS86 4.4 48.7 1.0 CB A:ASP170 4.5 44.5 1.0 NE2 A:HIS375 4.7 51.7 1.0 CB A:ASP85 4.7 50.3 1.0

J.Li, J.Hu, L.Bao, B.Zhan. Structural Ans Functional Study of Rnase J From Mycobacterium Tuberculosis To Be Published.