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Zinc in PDB 6p70: X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex

Enzymatic activity of X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex

All present enzymatic activity of X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex:
2.7.7.6;

Protein crystallography data

The structure of X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex, PDB code: 6p70 was solved by Y.Shin, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.06 / 3.05
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 185.179, 100.845, 294.865, 90.00, 98.81, 90.00
R / Rfree (%) 20.7 / 25.2

Other elements in 6p70:

The structure of X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex also contains other interesting chemical elements:

Magnesium (Mg) 3 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex (pdb code 6p70). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex, PDB code: 6p70:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 6p70

Go back to Zinc Binding Sites List in 6p70
Zinc binding site 1 out of 2 in the X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:96.3
occ:1.00
SG D:CYS1112 2.2 70.4 1.0
SG D:CYS1201 2.3 76.1 1.0
SG D:CYS1194 2.3 84.9 1.0
SG D:CYS1204 2.6 85.0 1.0
CB D:CYS1194 3.0 75.4 1.0
CB D:CYS1204 3.3 66.1 1.0
CA D:CYS1194 3.4 67.6 1.0
CB D:CYS1112 3.7 72.7 1.0
CB D:CYS1201 3.8 73.7 1.0
CG2 D:THR1114 4.0 86.8 1.0
N D:GLN1195 4.1 70.6 1.0
N D:CYS1204 4.1 70.1 1.0
NH2 D:ARG1189 4.1 83.4 1.0
OG1 D:THR1196 4.2 80.8 1.0
C D:CYS1194 4.3 69.2 1.0
CA D:CYS1204 4.3 69.2 1.0
N D:CYS1201 4.4 76.7 1.0
N D:CYS1112 4.4 85.1 1.0
N D:CYS1194 4.6 64.9 1.0
CA D:CYS1201 4.6 75.5 1.0
CA D:CYS1112 4.7 74.2 1.0
O D:CYS1201 4.9 72.3 1.0
OD1 D:ASP1111 4.9 88.3 1.0
N D:THR1196 4.9 73.0 1.0

Zinc binding site 2 out of 2 in 6p70

Go back to Zinc Binding Sites List in 6p70
Zinc binding site 2 out of 2 in the X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Crystal Structure of Bacterial Rna Polymerase and Pyrbi Promoter Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:0.8
occ:1.00
SG D:CYS76 2.1 0.9 1.0
SG D:CYS60 2.2 0.2 1.0
SG D:CYS73 2.3 0.8 1.0
SG D:CYS58 2.7 0.2 1.0
CB D:CYS76 3.1 1.0 1.0
CB D:CYS73 3.2 0.9 1.0
CB D:CYS58 3.5 0.6 1.0
N D:CYS76 3.6 0.4 1.0
N D:CYS60 3.7 0.2 1.0
CB D:CYS60 3.7 0.6 1.0
CA D:CYS76 3.9 0.7 1.0
N D:GLY61 4.1 0.7 1.0
CA D:CYS60 4.1 0.3 1.0
N D:LYS62 4.1 0.3 1.0
N D:ALA59 4.2 99.7 1.0
CB D:LYS62 4.3 0.6 1.0
C D:CYS60 4.5 0.7 1.0
C D:CYS58 4.6 0.0 1.0
CA D:CYS73 4.6 0.6 1.0
C D:CYS76 4.7 0.6 1.0
CA D:CYS58 4.7 0.8 1.0
C D:ALA59 4.7 0.7 1.0
C D:ARG75 4.7 0.9 1.0
N D:GLY77 4.8 0.3 1.0
CA D:LYS62 4.9 0.6 1.0
CB D:ARG75 4.9 0.9 1.0
CA D:ALA59 4.9 0.1 1.0

Reference:

Y.Shin, K.S.Murakami. Structural Basis of Reiterative Transcription From the Pyrg Promoter To Be Published.
Page generated: Tue Oct 29 04:53:03 2024

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