Zinc in PDB 6ow3: X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T

All present enzymatic activity of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T:
2.7.7.6;

The structure of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T, PDB code: 6ow3 was solved by Y.Shin, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.87 / 2.77
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	186.187, 101.833, 295.755, 90.00, 98.64, 90.00
R / Rfree (%)	21.7 / 25.5

The structure of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T (pdb code 6ow3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T, PDB code: 6ow3:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1604 b:0.8 occ:1.00 SG D:CYS73 2.2 0.8 1.0 SG D:CYS76 2.2 1.0 1.0 SG D:CYS60 2.3 0.3 1.0 SG D:CYS58 2.6 99.1 1.0 CB D:CYS76 3.2 0.4 1.0 CB D:CYS73 3.2 0.9 1.0 N D:CYS76 3.6 0.8 1.0 CB D:CYS58 3.6 0.3 1.0 N D:CYS60 3.7 0.4 1.0 CB D:CYS60 3.7 0.2 1.0 CA D:CYS76 4.0 0.9 1.0 CA D:CYS60 4.2 0.3 1.0 N D:GLY61 4.2 0.9 1.0 N D:LYS62 4.3 0.8 1.0 N D:ALA59 4.3 95.3 1.0 CB D:LYS62 4.4 0.4 1.0 C D:CYS60 4.5 0.5 1.0 CA D:CYS73 4.6 0.0 1.0 CB D:ARG75 4.6 1.0 1.0 C D:CYS58 4.7 0.9 1.0 C D:ARG75 4.7 0.8 1.0 CA D:CYS58 4.8 0.9 1.0 C D:ALA59 4.8 0.3 1.0 C D:CYS76 4.8 0.0 1.0 N D:GLY77 4.9 0.3 1.0 C D:CYS73 4.9 0.5 1.0 N D:ARG75 4.9 0.6 1.0 CA D:LYS62 5.0 0.6 1.0 CD2 D:TYR63 5.0 0.9 1.0 CA D:ARG75 5.0 0.4 1.0 CA D:ALA59 5.0 96.2 1.0

Zinc binding site 2 out of 2 in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1605 b:89.0 occ:1.00 SG D:CYS1204 2.3 78.1 1.0 SG D:CYS1194 2.4 83.8 1.0 SG D:CYS1112 2.4 77.3 1.0 SG D:CYS1201 2.4 76.5 1.0 CB D:CYS1204 3.1 66.5 1.0 CB D:CYS1194 3.2 65.5 1.0 CA D:CYS1194 3.5 62.6 1.0 CB D:CYS1112 3.7 67.2 1.0 CB D:CYS1201 3.7 65.1 1.0 N D:GLN1195 4.0 68.7 1.0 CG2 D:THR1114 4.0 74.0 1.0 OG1 D:THR1196 4.0 73.4 1.0 NH2 D:ARG1189 4.2 69.5 1.0 C D:CYS1194 4.2 65.5 1.0 N D:CYS1204 4.2 65.8 1.0 N D:CYS1201 4.2 73.6 1.0 CA D:CYS1204 4.3 64.8 1.0 N D:CYS1112 4.3 75.8 1.0 CA D:CYS1201 4.5 69.3 1.0 CA D:CYS1112 4.6 67.4 1.0 N D:CYS1194 4.6 59.4 1.0 N D:THR1196 4.7 64.6 1.0 O D:CYS1201 5.0 62.6 1.0

K.S.Murakami, Y.Shin. Structural Basis of Reiterative Transcription From the Pyrg Promoter To Be Published.