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Zinc in PDB 6l74: Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt

Enzymatic activity of Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt

All present enzymatic activity of Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt:
2.7.7.6;

Protein crystallography data

The structure of Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt, PDB code: 6l74 was solved by Y.Zhang, L.Li, R.H.Ebright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 44.92 / 3.12
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 183.071, 103.498, 295.128, 90.00, 99.17, 90.00
R / Rfree (%) 19.1 / 23.6

Other elements in 6l74:

The structure of Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt also contains other interesting chemical elements:

Magnesium (Mg) 5 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt (pdb code 6l74). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt, PDB code: 6l74:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 6l74

Go back to Zinc Binding Sites List in 6l74
Zinc binding site 1 out of 2 in the Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:99.8
occ:1.00
SG D:CYS1204 2.1 67.2 1.0
SG D:CYS1112 2.1 56.0 1.0
SG D:CYS1194 2.2 52.1 1.0
SG D:CYS1201 2.2 50.5 1.0
CB D:CYS1204 3.1 61.6 1.0
CB D:CYS1194 3.2 64.4 1.0
CA D:CYS1194 3.5 60.7 1.0
CB D:CYS1201 3.6 63.3 1.0
CB D:CYS1112 3.6 60.2 1.0
N D:GLN1195 4.0 50.4 1.0
NH2 D:ARG1189 4.1 57.0 1.0
OG1 D:THR1196 4.1 63.9 1.0
CG2 D:THR1114 4.1 58.7 1.0
N D:CYS1112 4.2 62.3 1.0
N D:CYS1201 4.2 54.0 1.0
C D:CYS1194 4.3 52.9 1.0
CA D:CYS1204 4.3 57.8 1.0
N D:CYS1204 4.3 58.9 1.0
O D:HOH2172 4.4 65.2 1.0
CA D:CYS1201 4.4 57.8 1.0
CA D:CYS1112 4.5 60.9 1.0
N D:CYS1194 4.6 50.0 1.0
N D:THR1196 4.7 53.5 1.0
NE D:ARG1189 4.9 61.6 1.0
OD1 D:ASP1111 4.9 69.0 1.0
CZ D:ARG1189 5.0 58.7 1.0
C D:CYS1201 5.0 55.3 1.0

Zinc binding site 2 out of 2 in 6l74

Go back to Zinc Binding Sites List in 6l74
Zinc binding site 2 out of 2 in the Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Thermus Thermophilus Initial Transcription Complex Comprising Sigma A and 5'-Triphosphate Rna of 2 Nt within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:0.3
occ:1.00
SG D:CYS76 2.3 0.7 1.0
SG D:CYS60 2.3 0.3 1.0
SG D:CYS73 2.4 0.7 1.0
SG D:CYS58 2.4 0.0 1.0
CB D:CYS73 3.2 0.5 1.0
CB D:CYS58 3.3 0.7 1.0
CB D:CYS76 3.9 0.8 1.0
CB D:CYS60 4.0 0.2 1.0
CB D:LYS62 4.0 0.7 1.0
N D:LYS62 4.0 0.4 1.0
N D:CYS60 4.1 0.9 1.0
N D:CYS76 4.2 0.8 1.0
N D:GLY61 4.2 0.9 1.0
CA D:CYS60 4.5 1.0 1.0
N D:ALA59 4.5 0.8 1.0
CA D:CYS58 4.6 0.0 1.0
CD2 D:TYR63 4.6 0.5 1.0
CA D:LYS62 4.6 0.6 1.0
CA D:CYS76 4.6 0.3 1.0
C D:CYS58 4.7 0.7 1.0
CA D:CYS73 4.7 0.4 1.0
C D:CYS60 4.8 0.5 1.0
CB D:ARG75 4.8 0.3 1.0
CE2 D:TYR63 4.9 0.0 1.0
N D:TYR63 4.9 0.1 1.0

Reference:

L.Li, V.Molodtsov, W.Lin, R.H.Ebright, Y.Zhang. Rna Extension Drives A Stepwise Displacement of An Initiation-Factor Structural Module in Initial Transcription. Proc.Natl.Acad.Sci.Usa 2020.
ISSN: ESSN 1091-6490
DOI: 10.1073/PNAS.1920747117
Page generated: Tue Oct 29 02:24:25 2024

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