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Zinc in PDB 6joo: Crystal Structure of Corynebacterium Diphtheriae CAS9 in Complex with Sgrna and Target Dna

Protein crystallography data

The structure of Crystal Structure of Corynebacterium Diphtheriae CAS9 in Complex with Sgrna and Target Dna, PDB code: 6joo was solved by S.Hirano, R.Ishitani, H.Nishimasu, O.Nureki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 67.95 / 2.90
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 139.015, 118.999, 116.272, 90.00, 113.59, 90.00
R / Rfree (%) 22.1 / 25.4

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Corynebacterium Diphtheriae CAS9 in Complex with Sgrna and Target Dna (pdb code 6joo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of Corynebacterium Diphtheriae CAS9 in Complex with Sgrna and Target Dna, PDB code: 6joo:

Zinc binding site 1 out of 1 in 6joo

Go back to Zinc Binding Sites List in 6joo
Zinc binding site 1 out of 1 in the Crystal Structure of Corynebacterium Diphtheriae CAS9 in Complex with Sgrna and Target Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Corynebacterium Diphtheriae CAS9 in Complex with Sgrna and Target Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1102

b:0.5
occ:1.00
ND1 A:HIS876 2.0 0.0 1.0
NE2 A:HIS869 2.0 0.3 1.0
CD2 A:HIS869 2.4 0.1 1.0
CE1 A:HIS876 2.5 0.9 1.0
CE1 A:HIS869 2.9 0.3 1.0
CG A:HIS869 3.3 0.7 1.0
CG A:HIS876 3.4 0.4 1.0
ND1 A:HIS869 3.5 0.8 1.0
NE2 A:HIS876 3.8 0.1 1.0
CB A:HIS876 4.1 0.0 1.0
CD2 A:HIS876 4.2 0.7 1.0
CB A:HIS869 4.5 1.0 1.0

Reference:

S.Hirano, O.O.Abudayyeh, J.S.Gootenberg, T.Horii, R.Ishitani, I.Hatada, F.Zhang, H.Nishimasu, O.Nureki. Structural Basis For the Promiscuous Pam Recognition By Corynebacterium Diphtheriae CAS9. Nat Commun V. 10 1968 2019.
ISSN: ESSN 2041-1723
PubMed: 31036811
DOI: 10.1038/S41467-019-09741-6
Page generated: Tue Oct 29 01:19:07 2024

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