Zinc in PDB 6jcx: Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna

All present enzymatic activity of Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna:
2.7.7.6;

The structure of Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna, PDB code: 6jcx was solved by L.Li, Y.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.09 / 2.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	125.139, 161.001, 128.846, 90.00, 117.06, 90.00
R / Rfree (%)	21 / 25.6

The structure of Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna (pdb code 6jcx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna, PDB code: 6jcx:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2001 b:71.9 occ:1.00 SG D:CYS975 2.2 67.0 1.0 SG D:CYS978 2.3 67.8 1.0 SG D:CYS968 2.3 69.2 1.0 SG D:CYS891 2.4 83.0 1.0 CB D:CYS978 2.8 65.1 1.0 CB D:CYS968 3.1 66.1 1.0 CA D:CYS968 3.4 64.9 1.0 CB D:CYS975 3.5 69.7 1.0 CB D:CYS891 3.6 72.0 1.0 N D:ALA969 3.9 71.9 1.0 N D:CYS975 3.9 66.6 1.0 NH1 D:ARG963 4.0 67.5 1.0 CA D:CYS978 4.1 57.4 1.0 C D:CYS968 4.1 72.2 1.0 OG1 D:THR970 4.1 65.7 1.0 N D:CYS978 4.2 54.5 1.0 CA D:CYS975 4.2 65.0 1.0 N D:CYS891 4.3 69.3 1.0 O D:CYS975 4.5 57.7 1.0 CA D:CYS891 4.6 74.0 1.0 N D:THR970 4.6 79.7 1.0 N D:CYS968 4.7 64.7 1.0 C D:CYS975 4.7 59.0 1.0 CG2 D:THR893 4.7 72.1 1.0

Zinc binding site 2 out of 2 in the Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mycobacterium Tuberculosis Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 6NT Rna within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:91.4 occ:1.00 SG D:CYS62 2.2 92.9 1.0 SG D:CYS78 2.2 78.8 1.0 SG D:CYS75 2.2 73.1 1.0 SG D:CYS60 2.3 87.8 1.0 CB D:CYS78 3.2 83.6 1.0 CB D:CYS62 3.4 87.4 1.0 CB D:CYS75 3.4 74.6 1.0 N D:CYS62 3.5 85.5 1.0 CB D:CYS60 3.6 78.1 1.0 N D:CYS78 3.8 82.0 1.0 CA D:CYS62 3.9 87.5 1.0 CB D:LYS64 4.0 87.4 1.0 N D:LYS64 4.0 92.0 1.0 N D:GLY63 4.1 90.7 1.0 CA D:CYS78 4.2 83.4 1.0 C D:CYS62 4.3 90.9 1.0 N D:TYR61 4.3 81.5 1.0 CB D:ARG77 4.5 91.8 1.0 C D:CYS60 4.6 84.1 1.0 CA D:LYS64 4.6 91.0 1.0 C D:TYR61 4.7 82.0 1.0 C D:ARG77 4.7 78.4 1.0 CD2 D:TYR65 4.7 92.1 1.0 CA D:CYS60 4.7 75.7 1.0 CA D:CYS75 4.8 78.2 1.0 CA D:ARG77 5.0 81.6 1.0 N D:ARG77 5.0 77.6 1.0 CE2 D:TYR65 5.0 94.5 1.0 CA D:TYR61 5.0 80.5 1.0

C.Fang, L.Li, L.Shen, J.Shi, S.Wang, Y.Feng, Y.Zhang. Structures and Mechanism of Transcription Initiation By Bacterial Ecf Factors. Nucleic Acids Res. V. 47 7094 2019.
ISSN: ESSN 1362-4962
PubMed: 31131408
DOI: 10.1093/NAR/GKZ470