Zinc in PDB 6fn8: Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution

The structure of Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution, PDB code: 6fn8 was solved by F.A.Sala, G.S.A.Wright, S.V.Antonyuk, R.C.Garratt, S.S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	61.09 / 1.55
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	68.359, 68.359, 136.180, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 21.7

The binding sites of Zinc atom in the Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution (pdb code 6fn8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution, PDB code: 6fn8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:11.0 occ:1.00 OD1 A:ASP167 2.0 9.4 1.0 ND1 A:HIS164 2.1 12.4 1.0 ND1 A:HIS147 2.1 9.8 1.0 ND1 A:HIS155 2.1 9.1 1.0 CG A:ASP167 2.7 11.4 1.0 OD2 A:ASP167 2.8 11.0 1.0 CE1 A:HIS155 2.9 10.0 1.0 CE1 A:HIS164 2.9 11.6 1.0 CE1 A:HIS147 3.0 9.7 1.0 CG A:HIS147 3.1 10.2 1.0 CG A:HIS164 3.1 12.1 1.0 CG A:HIS155 3.2 10.2 1.0 CB A:HIS147 3.4 10.2 1.0 CB A:HIS164 3.5 11.6 1.0 CB A:HIS155 3.7 10.7 1.0 CA A:HIS155 4.0 11.8 1.0 NE2 A:HIS155 4.1 10.4 1.0 NE2 A:HIS164 4.1 11.3 1.0 NE2 A:HIS147 4.1 11.2 1.0 CB A:ASP167 4.2 11.0 1.0 CD2 A:HIS164 4.2 13.4 1.0 CD2 A:HIS147 4.2 9.7 1.0 CD2 A:HIS155 4.2 10.8 1.0 O A:ILE217 4.3 15.4 1.0 CD2 A:HIS130 4.6 13.7 1.0 CA A:ASP167 4.7 10.3 1.0 N A:HIS164 4.8 12.3 1.0 CA A:HIS164 4.8 11.6 1.0 N A:GLY156 4.8 12.4 1.0 C A:HIS155 4.9 13.1 1.0 O A:THR218 4.9 13.7 1.0 CA A:HIS147 5.0 10.9 1.0 N A:HIS155 5.0 13.2 1.0

Zinc binding site 2 out of 2 in the Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Domain II of the Human Copper Chaperone For Superoxide Dismutase at 1.55 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:11.9 occ:1.00 ND1 B:HIS164 2.0 12.6 1.0 OD1 B:ASP167 2.0 12.9 1.0 ND1 B:HIS147 2.0 10.9 1.0 ND1 B:HIS155 2.1 11.5 1.0 CG B:ASP167 2.8 13.7 1.0 OD2 B:ASP167 2.9 12.3 1.0 CE1 B:HIS155 2.9 12.7 1.0 CE1 B:HIS164 2.9 14.3 1.0 CE1 B:HIS147 3.0 9.7 1.0 CG B:HIS147 3.1 11.9 1.0 CG B:HIS164 3.1 11.4 1.0 CG B:HIS155 3.2 14.0 1.0 CB B:HIS147 3.4 11.5 1.0 CB B:HIS164 3.5 11.4 1.0 CB B:HIS155 3.7 13.2 1.0 CA B:HIS155 4.0 12.9 1.0 NE2 B:HIS164 4.0 11.9 1.0 NE2 B:HIS155 4.1 12.3 1.0 NE2 B:HIS147 4.1 11.3 1.0 CD2 B:HIS164 4.1 12.6 1.0 O B:ILE217 4.1 17.1 1.0 CD2 B:HIS147 4.2 11.1 1.0 CB B:ASP167 4.2 11.7 1.0 CD2 B:HIS155 4.2 13.1 1.0 CD2 B:HIS130 4.6 13.2 1.0 CA B:ASP167 4.7 10.7 1.0 N B:HIS164 4.7 11.3 1.0 CA B:HIS164 4.8 12.1 1.0 N B:GLY156 4.8 15.8 1.0 O B:HOH485 4.9 25.8 1.0 C B:HIS155 4.9 16.4 1.0 CA B:HIS147 4.9 12.5 1.0 N B:HIS155 5.0 14.7 1.0 N B:ASP167 5.0 10.1 1.0 O B:THR218 5.0 16.4 1.0

F.A.Sala, G.S.A.Wright, S.V.Antonyuk, R.C.Garratt, S.S.Hasnain. Molecular Recognition and Maturation of SOD1 By Its Evolutionarily Destabilised Cognate Chaperone Hccs. Plos Biol. V. 17 00141 2019.
ISSN: ESSN 1545-7885
PubMed: 30735496
DOI: 10.1371/JOURNAL.PBIO.3000141