Zinc in PDB 5zx3: Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H

All present enzymatic activity of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H:
2.7.7.6;

The structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H, PDB code: 5zx3 was solved by L.Li, Y.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.64 / 2.75
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	130.572, 159.790, 131.437, 90.00, 118.72, 90.00
R / Rfree (%)	21.8 / 25.8

The binding sites of Zinc atom in the Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H (pdb code 5zx3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H, PDB code: 5zx3:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2001 b:89.4 occ:1.00 SG D:CYS975 2.2 66.6 1.0 SG D:CYS968 2.3 76.5 1.0 SG D:CYS978 2.3 82.3 1.0 SG D:CYS891 2.4 94.5 1.0 CB D:CYS978 3.1 80.7 1.0 CB D:CYS968 3.1 80.9 1.0 CB D:CYS975 3.4 81.0 1.0 CA D:CYS968 3.4 81.6 1.0 NH2 D:ARG963 3.7 86.0 1.0 CB D:CYS891 3.7 80.1 1.0 N D:ALA969 3.7 90.0 1.0 N D:CYS975 3.9 77.8 1.0 C D:CYS968 4.1 83.4 1.0 OG1 D:THR970 4.1 84.9 1.0 CA D:CYS975 4.2 77.8 1.0 CG2 D:THR893 4.3 91.5 1.0 CA D:CYS978 4.3 78.4 1.0 N D:CYS978 4.3 72.4 1.0 N D:CYS891 4.4 82.8 1.0 O D:CYS975 4.6 67.0 1.0 N D:THR970 4.6 86.8 1.0 CA D:CYS891 4.7 81.0 1.0 N D:CYS968 4.7 82.8 1.0 C D:CYS975 4.8 72.1 1.0 CZ D:ARG963 4.8 83.3 1.0 CA D:ALA969 4.9 92.4 1.0

Zinc binding site 2 out of 2 in the Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme with Ecf Sigma Factor Sigma H within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:87.2 occ:1.00 SG D:CYS62 2.2 92.1 1.0 SG D:CYS75 2.3 83.7 1.0 SG D:CYS78 2.3 95.5 1.0 SG D:CYS60 2.4 92.6 1.0 CB D:CYS75 3.2 86.1 1.0 CB D:CYS60 3.2 87.4 1.0 CB D:CYS62 3.3 91.2 1.0 N D:CYS62 3.5 92.3 1.0 CB D:CYS78 3.6 91.8 1.0 N D:CYS78 3.8 0.2 1.0 CA D:CYS62 3.9 95.0 1.0 N D:GLY63 4.1 94.0 1.0 N D:TYR61 4.2 80.4 1.0 CA D:CYS78 4.3 92.9 1.0 N D:LYS64 4.4 96.0 1.0 CB D:ARG77 4.4 0.1 1.0 C D:CYS60 4.4 82.8 1.0 C D:CYS62 4.4 94.7 1.0 CA D:CYS60 4.4 85.8 1.0 CB D:LYS64 4.5 97.5 1.0 C D:TYR61 4.6 88.6 1.0 CA D:CYS75 4.7 89.8 1.0 CD1 D:TYR65 4.8 99.0 1.0 C D:ARG77 4.9 0.1 1.0 CA D:TYR61 5.0 84.4 1.0 CA D:ARG77 5.0 96.0 1.0 N D:ARG77 5.0 90.5 1.0 C D:CYS78 5.0 87.6 1.0 O D:CYS60 5.0 82.0 1.0

L.Li, C.Fang, N.Zhuang, T.Wang, Y.Zhang. Structural Basis For Transcription Initiation By Bacterial Ecf Sigma Factors. Nat Commun V. 10 1153 2019.
ISSN: ESSN 2041-1723
PubMed: 30858373
DOI: 10.1038/S41467-019-09096-Y