Zinc in PDB 5wck: Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii

All present enzymatic activity of Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii:
3.5.2.6;

The structure of Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii, PDB code: 5wck was solved by I.Garcia-Saez, P.S.Mercuri, R.Kahn, C.Papamicael, I.G.Shabalin, J.E.Raczynska, M.Jaskolski, W.Minor, J.M.Frere, M.Galleni, O.Dideberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.93 / 1.65
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	44.860, 76.850, 78.890, 90.00, 102.08, 90.00
R / Rfree (%)	12.3 / 15.8

The binding sites of Zinc atom in the Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii (pdb code 5wck). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii, PDB code: 5wck:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:11.2 occ:1.00 O A:HOH580 2.0 18.7 1.0 OD2 A:ASP94 2.0 9.5 1.0 NE2 A:HIS234 2.1 7.2 1.0 NE2 A:HIS95 2.1 6.2 1.0 UNK A:UNX313 2.2 16.4 1.0 UNK A:UNX312 2.3 17.6 1.0 CG A:ASP94 3.0 8.4 1.0 CE1 A:HIS234 3.0 9.9 1.0 CE1 A:HIS95 3.0 6.1 1.0 CD2 A:HIS234 3.1 9.0 1.0 CD2 A:HIS95 3.1 6.1 1.0 OD1 A:ASP94 3.3 9.3 1.0 UNK A:UNX309 3.4 11.5 1.0 ZN A:ZN302 3.7 8.5 1.0 NE2 A:HIS90 4.1 6.9 1.0 CE1 A:HIS90 4.1 7.9 1.0 ND1 A:HIS234 4.1 8.1 1.0 UNK A:UNX310 4.1 16.5 1.0 ND1 A:HIS95 4.2 6.9 1.0 UNK A:UNX314 4.2 16.4 1.0 CG A:HIS234 4.2 7.7 1.0 CG A:HIS95 4.2 5.0 1.0 CB A:ASP94 4.3 9.7 1.0 O A:HOH552 4.7 32.3 1.0 UNK A:UNX311 4.8 41.6 1.0 OG A:SER233 5.0 6.8 1.0 NE2 A:HIS168 5.0 5.8 1.0

Zinc binding site 2 out of 4 in the Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn302 b:8.5 occ:1.00 O A:HOH580 2.0 18.7 1.0 ND1 A:HIS92 2.1 8.1 1.0 NE2 A:HIS168 2.1 5.8 1.0 NE2 A:HIS90 2.1 6.9 1.0 UNK A:UNX310 2.4 16.5 1.0 CE1 A:HIS92 3.0 6.5 1.0 CE1 A:HIS90 3.0 7.9 1.0 CD2 A:HIS168 3.0 5.3 1.0 CD2 A:HIS90 3.1 6.5 1.0 CE1 A:HIS168 3.1 6.3 1.0 CG A:HIS92 3.1 7.2 1.0 UNK A:UNX313 3.3 16.4 1.0 CB A:HIS92 3.5 6.3 1.0 UNK A:UNX309 3.6 11.5 1.0 ZN A:ZN301 3.7 11.2 1.0 ND1 A:HIS90 4.1 5.3 1.0 NE2 A:HIS92 4.1 8.4 1.0 CG A:HIS90 4.2 6.1 1.0 UNK A:UNX312 4.2 17.6 1.0 OD1 A:ASP94 4.2 9.3 1.0 CG A:HIS168 4.2 6.1 1.0 ND1 A:HIS168 4.2 6.2 1.0 CD2 A:HIS92 4.2 8.8 1.0 CD2 A:HIS95 4.3 6.1 1.0 NE2 A:HIS95 4.4 6.2 1.0 UNK A:UNX311 4.4 41.6 1.0 UNK A:UNX314 4.5 16.4 1.0 ND2 A:ASN197 4.6 11.2 1.0 OD2 A:ASP94 4.8 9.5 1.0 CG A:ASP94 4.9 8.4 1.0 CG2 A:THR169 5.0 7.5 1.0 CA A:HIS92 5.0 7.1 1.0

Zinc binding site 3 out of 4 in the Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:11.5 occ:1.00 O B:HOH549 2.0 20.8 1.0 OD2 B:ASP94 2.0 10.6 1.0 NE2 B:HIS234 2.1 7.5 1.0 NE2 B:HIS95 2.1 6.3 1.0 UNK B:UNX310 2.3 15.2 1.0 UNK B:UNX309 2.3 15.2 1.0 CG B:ASP94 3.0 9.3 1.0 CE1 B:HIS234 3.0 9.9 1.0 CE1 B:HIS95 3.1 7.3 1.0 CD2 B:HIS234 3.1 8.7 1.0 CD2 B:HIS95 3.1 7.5 1.0 OD1 B:ASP94 3.2 10.4 1.0 UNK B:UNX312 3.4 14.1 1.0 ZN B:ZN302 3.7 8.9 1.0 NE2 B:HIS90 4.0 6.9 1.0 UNK B:UNX314 4.0 40.9 1.0 CE1 B:HIS90 4.1 7.0 1.0 ND1 B:HIS234 4.2 7.9 1.0 ND1 B:HIS95 4.2 7.7 1.0 CG B:HIS95 4.2 5.5 1.0 CG B:HIS234 4.2 7.6 1.0 UNK B:UNX311 4.2 17.7 1.0 UNK B:UNX313 4.2 14.8 1.0 CB B:ASP94 4.3 9.2 1.0 O B:HOH648 4.6 33.0 1.0 NE2 B:HIS168 5.0 6.8 1.0 OG B:SER233 5.0 8.5 1.0

Zinc binding site 4 out of 4 in the Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Native Fez-1 Metallo-Beta-Lactamase From Legionella Gormanii within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn302 b:8.9 occ:1.00 O B:HOH549 2.0 20.8 1.0 ND1 B:HIS92 2.1 7.2 1.0 NE2 B:HIS168 2.1 6.8 1.0 NE2 B:HIS90 2.1 6.9 1.0 UNK B:UNX313 2.4 14.8 1.0 CE1 B:HIS92 3.0 6.9 1.0 CD2 B:HIS168 3.0 5.6 1.0 CD2 B:HIS90 3.0 5.5 1.0 CE1 B:HIS90 3.0 7.0 1.0 CE1 B:HIS168 3.1 7.7 1.0 CG B:HIS92 3.1 6.3 1.0 UNK B:UNX310 3.3 15.2 1.0 UNK B:UNX312 3.5 14.1 1.0 CB B:HIS92 3.5 6.0 1.0 ZN B:ZN301 3.7 11.5 1.0 ND1 B:HIS90 4.1 7.1 1.0 NE2 B:HIS92 4.1 9.6 1.0 CG B:HIS90 4.1 6.5 1.0 UNK B:UNX309 4.2 15.2 1.0 OD1 B:ASP94 4.2 10.4 1.0 CG B:HIS168 4.2 5.8 1.0 CD2 B:HIS92 4.2 8.8 1.0 ND1 B:HIS168 4.2 7.7 1.0 CD2 B:HIS95 4.3 7.5 1.0 O B:HOH726 4.3 36.9 1.0 NE2 B:HIS95 4.4 6.3 1.0 UNK B:UNX311 4.6 17.7 1.0 ND2 B:ASN197 4.6 12.5 1.0 UNK B:UNX314 4.7 40.9 1.0 OD2 B:ASP94 4.8 10.6 1.0 CG B:ASP94 4.9 9.3 1.0 CG2 B:THR169 4.9 7.8 1.0 CA B:HIS92 5.0 6.6 1.0

I.Garcia-Saez, P.S.Mercuri, C.Papamicael, R.Kahn, J.M.Frere, M.Galleni, G.M.Rossolini, O.Dideberg. Three-Dimensional Structure of Fez-1, A Monomeric Subclass B3 Metallo-Beta-Lactamase From Fluoribacter Gormanii, in Native Form and in Complex with D-Captopril. J. Mol. Biol. V. 325 651 2003.
ISSN: ISSN 0022-2836
PubMed: 12507470