Zinc in PDB 5nwt: Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex

All present enzymatic activity of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex:
2.7.7.6;

The structure of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex, PDB code: 5nwt was solved by X.Zhang, M.Buck, V.C.Darbari, Y.Yang, N.Zhang, D.Lu, R.Glyde, Y.Wang, J.Winkelman, R.L.Gourse, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	195.28 / 3.76
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	208.477, 151.525, 195.279, 90.00, 90.00, 90.00
R / Rfree (%)	31.3 / 37.1

The structure of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex (pdb code 5nwt). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex, PDB code: 5nwt:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1501 b:0.5 occ:1.00 SG D:CYS72 1.8 1.0 1.0 SG D:CYS85 2.3 0.3 1.0 SG D:CYS70 2.5 0.8 1.0 CB D:CYS85 3.1 0.9 1.0 CB D:CYS88 3.4 0.4 1.0 CB D:CYS72 3.5 0.6 1.0 CB D:CYS70 3.5 0.3 1.0 N D:CYS72 4.0 1.0 1.0 N D:CYS88 4.1 0.5 1.0 CB D:LYS74 4.2 0.4 1.0 N D:GLY73 4.2 0.7 1.0 CA D:CYS72 4.2 0.7 1.0 CA D:CYS88 4.3 0.3 1.0 N D:LYS74 4.3 0.9 1.0 CA D:CYS85 4.4 0.9 1.0 SG D:CYS88 4.6 0.1 1.0 C D:CYS72 4.7 0.7 1.0 N D:LEU71 4.7 0.4 1.0 CA D:CYS70 4.8 0.2 1.0 O D:CYS88 4.8 1.0 1.0 C D:CYS88 4.8 0.3 1.0 CA D:LYS74 4.9 0.6 1.0 C D:CYS70 5.0 0.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1503 b:0.7 occ:1.00 SG D:CYS888 2.1 0.2 1.0 SG D:CYS895 2.1 0.8 1.0 SG D:CYS814 2.6 0.1 1.0 CB D:CYS888 3.2 0.7 1.0 CB D:CYS814 3.3 0.5 1.0 CB D:CYS895 3.4 0.5 1.0 CA D:CYS888 3.7 0.8 1.0 SG D:CYS898 3.8 0.9 1.0 N D:CYS895 4.1 0.9 1.0 N D:ASP889 4.2 0.8 1.0 CB D:CYS898 4.2 0.8 1.0 C D:CYS888 4.3 0.9 1.0 CA D:CYS895 4.4 0.7 1.0 N D:CYS814 4.5 1.0 1.0 CA D:CYS814 4.6 0.1 1.0 N D:THR890 4.6 0.1 1.0 O D:SER887 4.6 0.0 1.0 CB D:THR890 4.7 0.9 1.0 N D:CYS888 4.9 0.1 1.0

Y.Yang, V.C.Darbari, N.Zhang, D.Lu, R.Glyde, Y.P.Wang, J.T.Winkelman, R.L.Gourse, K.S.Murakami, M.Buck, X.Zhang. Transcription. Structures of the Rna Polymerase-SIGMA54 Reveal New and Conserved Regulatory Strategies. Science V. 349 882 2015.
ISSN: ESSN 1095-9203
PubMed: 26293966
DOI: 10.1126/SCIENCE.AAB1478