Zinc in PDB 5emi: N-Acetylmuramoyl-L-Alanine Amidase AMIC2 of Nostoc Punctiforme

All present enzymatic activity of N-Acetylmuramoyl-L-Alanine Amidase AMIC2 of Nostoc Punctiforme:
3.5.1.28;

The structure of N-Acetylmuramoyl-L-Alanine Amidase AMIC2 of Nostoc Punctiforme, PDB code: 5emi was solved by F.M.Buettner, T.Stehle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.25 / 1.12
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	35.030, 62.760, 65.290, 90.00, 90.00, 90.00
R / Rfree (%)	13.4 / 14.6

The binding sites of Zinc atom in the N-Acetylmuramoyl-L-Alanine Amidase AMIC2 of Nostoc Punctiforme (pdb code 5emi). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the N-Acetylmuramoyl-L-Alanine Amidase AMIC2 of Nostoc Punctiforme, PDB code: 5emi:

Zinc binding site 1 out of 1 in the N-Acetylmuramoyl-L-Alanine Amidase AMIC2 of Nostoc Punctiforme


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of N-Acetylmuramoyl-L-Alanine Amidase AMIC2 of Nostoc Punctiforme within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:6.5 occ:1.00 OE2 A:GLU462 2.1 6.2 1.0 ND1 A:HIS515 2.1 6.3 1.0 NE2 A:HIS447 2.1 6.8 1.0 OE1 A:GLU462 2.7 6.8 1.0 CD A:GLU462 2.7 6.3 1.0 CE1 A:HIS515 3.0 6.2 1.0 CD2 A:HIS447 3.0 6.6 1.0 CE1 A:HIS447 3.1 7.0 1.0 CG A:HIS515 3.1 5.8 1.0 CB A:HIS515 3.5 5.6 1.0 O A:ALA516 4.0 7.4 1.0 NE2 A:HIS515 4.1 6.6 1.0 C2 A:MES704 4.1 15.0 0.7 CG A:GLU462 4.2 7.4 1.0 ND1 A:HIS447 4.2 7.0 1.0 CG A:HIS447 4.2 6.2 1.0 CD2 A:HIS515 4.2 6.3 1.0 CA A:HIS515 4.3 5.6 1.0 N A:ALA516 4.5 6.4 1.0 OE2 A:GLU578 4.5 11.6 1.0 O A:HOH907 4.6 32.9 1.0 O1 A:MES704 4.7 15.9 0.7 C A:HIS515 5.0 6.2 1.0 OE1 A:GLU578 5.0 10.8 1.0 C A:ALA516 5.0 6.9 1.0

F.M.Buttner, K.Faulhaber, K.Forchhammer, I.Maldener, T.Stehle. Enabling Cell-Cell Communication Via Nanopore Formation: Structure, Function and Localization of the Unique Cell Wall Amidase AMIC2 of Nostoc Punctiforme. Febs J. V. 283 1336 2016.
ISSN: ISSN 1742-464X
PubMed: 26833702
DOI: 10.1111/FEBS.13673