Atomistry » Zinc » PDB 4wh8-4wwl » 4wqt
Atomistry »
  Zinc »
    PDB 4wh8-4wwl »
      4wqt »

Zinc in PDB 4wqt: Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein)

Enzymatic activity of Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein)

All present enzymatic activity of Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein):
2.7.7.6;

Protein crystallography data

The structure of Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein), PDB code: 4wqt was solved by Y.Murayama, S.Sekine, S.Yokoyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.79 / 4.40
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 189.568, 263.770, 195.848, 90.00, 116.83, 90.00
R / Rfree (%) 26 / 31.3

Other elements in 4wqt:

The structure of Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein) also contains other interesting chemical elements:

Magnesium (Mg) 3 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein) (pdb code 4wqt). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein), PDB code: 4wqt:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in 4wqt

Go back to Zinc Binding Sites List in 4wqt
Zinc binding site 1 out of 3 in the Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:0.9
occ:1.00
 N D:CYS1201 2.2 0.7 1.0
CB D:CYS1201 2.3 0.2 1.0
CA D:CYS1201 2.7 0.7 1.0
SG D:CYS1194 3.0 0.6 1.0
CG1 D:VAL1200 3.1 0.4 1.0
SG D:CYS1112 3.2 0.9 1.0
CB D:CYS1204 3.3 1.0 1.0
C D:VAL1200 3.3 0.6 1.0
CB D:CYS1112 3.4 0.9 1.0
CA D:VAL1200 3.6 0.6 1.0
CB D:CYS1194 3.7 0.6 1.0
C D:CYS1201 3.7 0.7 1.0
OG1 D:THR1196 3.8 0.9 1.0
SG D:CYS1204 3.8 1.0 1.0
O D:CYS1201 3.9 0.7 1.0
SG D:CYS1201 3.9 0.2 1.0
CB D:VAL1200 4.0 0.4 1.0
O D:VAL1200 4.3 0.6 1.0
N D:CYS1204 4.4 0.5 1.0
CB D:THR1196 4.5 0.9 1.0
CA D:CYS1204 4.5 0.5 1.0
CA D:CYS1194 4.5 0.6 1.0
CA D:CYS1112 4.5 0.8 1.0
N D:CYS1112 4.6 0.8 1.0
CG D:GLU1109 4.8 0.8 1.0
N D:GLN1202 4.9 0.3 1.0

Zinc binding site 2 out of 3 in 4wqt

Go back to Zinc Binding Sites List in 4wqt
Zinc binding site 2 out of 3 in the Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein) within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn1601

b:0.2
occ:1.01
 SG I:CYS1194 1.9 0.7 1.0
CB I:CYS1204 2.0 1.0 1.0
SG I:CYS1204 2.1 1.0 1.0
CG1 I:VAL1200 2.3 0.2 1.0
CB I:CYS1194 2.4 0.7 1.0
SG I:CYS1112 2.5 0.3 1.0
N I:CYS1201 3.3 0.8 1.0
SG I:CYS1201 3.3 0.3 1.0
CA I:CYS1204 3.6 0.7 1.0
CB I:VAL1200 3.6 0.2 1.0
CA I:CYS1194 3.8 0.6 1.0
CA I:VAL1200 3.9 0.6 1.0
CB I:CYS1201 4.1 0.3 1.0
C I:VAL1200 4.1 0.6 1.0
N I:CYS1204 4.2 0.7 1.0
CA I:CYS1201 4.2 0.8 1.0
CB I:CYS1112 4.2 0.3 1.0
C I:CYS1204 4.3 0.7 1.0
O I:CYS1201 4.4 0.8 1.0
CA I:PRO1191 4.6 0.6 1.0
CG2 I:VAL1200 4.6 0.2 1.0
N I:TYR1205 4.6 0.0 1.0
C I:CYS1201 4.7 0.8 1.0
N I:CYS1194 4.7 0.6 1.0
C I:CYS1194 4.7 0.6 1.0

Zinc binding site 3 out of 3 in 4wqt

Go back to Zinc Binding Sites List in 4wqt
Zinc binding site 3 out of 3 in the Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Thermus Thermophilus Rna Polymerase Complexed with An Rna Cleavage Stimulating Factor (A Grea/GFH1 Chimeric Protein) within 5.0Å range:
probe atom residue distance (Å) B Occ
N:Zn1601

b:0.1
occ:1.00
 SG N:CYS1201 2.3 1.0 1.0
SG N:CYS1112 2.6 0.5 1.0
SG N:CYS1204 2.8 0.1 1.0
CB N:CYS1204 2.9 0.1 1.0
CB N:CYS1112 3.0 0.5 1.0
CA N:CYS1194 3.0 0.3 1.0
CB N:CYS1194 3.1 0.0 1.0
SG N:CYS1194 3.4 0.0 1.0
N N:GLN1195 3.9 0.9 1.0
C N:CYS1194 3.9 0.3 1.0
N N:CYS1194 4.1 0.3 1.0
CB N:CYS1201 4.1 1.0 1.0
CA N:CYS1204 4.3 0.2 1.0
CA N:CYS1112 4.4 0.9 1.0
O N:THR1193 4.5 0.1 1.0
N N:CYS1201 4.5 1.0 1.0
N N:CYS1204 4.6 0.2 1.0
C N:THR1193 4.6 0.1 1.0
OG1 N:THR1196 4.7 0.4 1.0
CG2 N:THR1114 4.8 0.8 1.0
N N:CYS1112 4.8 0.9 1.0
CA N:CYS1201 4.9 1.0 1.0
N N:THR1196 5.0 0.5 1.0

Reference:

S.I.Sekine, Y.Murayama, V.Svetlov, E.Nudler, S.Yokoyama. The Ratcheted and Ratchetable Structural States of Rna Polymerase Underlie Multiple Transcriptional Functions. Mol.Cell V. 57 408 2015.
ISSN: ISSN 1097-2765
PubMed: 25601758
DOI: 10.1016/J.MOLCEL.2014.12.014
Page generated: Wed Aug 20 23:09:55 2025

Last articles

Zn in 9UUO
Zn in 9UUS
Zn in 9W4R
Zn in 9VKW
Zn in 9W4S
Zn in 9VH1
Zn in 9RMX
Zn in 9RMU
Zn in 9QWN
Zn in 9U9Y
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy