Zinc in PDB 4u8h: Crystal Structure of Mammalian Period-Cryptochrome Complex

The structure of Crystal Structure of Mammalian Period-Cryptochrome Complex, PDB code: 4u8h was solved by S.N.Nangle, C.Rosensweig, N.Koike, H.Tei, J.S.Takahashi, C.B.Green, N.Zheng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.74 / 2.80
Space group	P 41
Cell size a, b, c (Å), α, β, γ (°)	97.675, 97.675, 163.213, 90.00, 90.00, 90.00
R / Rfree (%)	20.5 / 27.8

The binding sites of Zinc atom in the Crystal Structure of Mammalian Period-Cryptochrome Complex (pdb code 4u8h). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Mammalian Period-Cryptochrome Complex, PDB code: 4u8h:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Mammalian Period-Cryptochrome Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Mammalian Period-Cryptochrome Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2000 b:0.6 occ:1.00 SG B:CYS1210 2.4 0.7 1.0 CD2 A:HIS491 2.5 96.3 1.0 NE2 A:HIS491 2.6 0.6 1.0 SG A:CYS432 2.7 0.6 1.0 N B:CYS1213 3.0 0.8 1.0 CB A:CYS432 3.5 0.2 1.0 N B:TYR1212 3.5 0.5 1.0 C B:TYR1212 3.7 1.0 1.0 CA B:TYR1212 3.8 0.0 1.0 CG A:HIS491 3.8 0.6 1.0 CB B:CYS1213 3.8 0.9 1.0 CE1 A:HIS491 3.9 0.1 1.0 CB B:TYR1212 3.9 0.9 1.0 CA B:CYS1213 3.9 0.6 1.0 CB B:CYS1210 4.2 0.6 1.0 SG B:CYS1213 4.2 99.1 1.0 CA A:CYS432 4.3 0.9 1.0 ND1 A:HIS491 4.5 0.3 1.0 N B:VAL1211 4.5 0.1 1.0 O B:TYR1212 4.7 0.6 1.0 C B:VAL1211 4.8 0.0 1.0 C B:CYS1213 4.9 0.6 1.0 CB A:HIS491 4.9 0.8 1.0 CD2 B:TYR1212 4.9 0.9 1.0 CG B:TYR1212 5.0 0.4 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Mammalian Period-Cryptochrome Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Mammalian Period-Cryptochrome Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2000 b:0.7 occ:1.00 SG D:CYS1213 1.2 0.7 1.0 NE2 C:HIS491 1.9 88.9 1.0 CE1 C:HIS491 2.6 86.5 1.0 SG D:CYS1210 2.6 0.3 1.0 CB D:CYS1213 2.7 0.5 1.0 SG C:CYS432 3.0 0.2 1.0 CD2 C:HIS491 3.1 90.4 1.0 N D:CYS1213 3.1 0.0 1.0 CB C:CYS432 3.3 82.5 1.0 CA D:CYS1213 3.4 0.5 1.0 CB D:CYS1210 3.5 0.0 1.0 C D:TYR1212 3.5 0.1 1.0 CD2 D:TYR1212 3.7 0.6 1.0 ND1 C:HIS491 3.8 90.0 1.0 CB D:TYR1212 3.8 0.5 1.0 CA C:CYS432 4.0 97.3 1.0 CG C:HIS491 4.0 91.2 1.0 O D:TYR1212 4.1 0.1 1.0 CA D:TYR1212 4.1 0.2 1.0 CG D:TYR1212 4.2 0.2 1.0 N D:TYR1212 4.4 0.3 1.0 C D:CYS1210 4.6 0.3 1.0 O D:CYS1210 4.6 0.6 1.0 CA D:CYS1210 4.7 0.6 1.0 CD C:PRO433 4.7 72.8 1.0 CE2 D:TYR1212 4.7 0.3 1.0 C D:CYS1213 4.8 0.2 1.0 C D:VAL1211 4.9 0.8 1.0 N C:CYS432 5.0 0.1 1.0

S.N.Nangle, C.Rosensweig, N.Koike, H.Tei, J.S.Takahashi, C.B.Green, N.Zheng. Molecular Assembly of the Period-Cryptochrome Circadian Transcriptional Repressor Complex. Elife V. 3 03674 2014.
ISSN: ESSN 2050-084X
PubMed: 25127877
DOI: 10.7554/ELIFE.03674