Zinc in PDB 4p8q: Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site

All present enzymatic activity of Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site:
3.4.11.3;

The structure of Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site, PDB code: 4p8q was solved by S.J.Hermans, D.B.Ascher, N.C.Hancock, J.K.Holien, B.Michell, C.J.Morton, M.W.Parker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.42 / 3.02
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	68.323, 256.051, 71.132, 90.00, 115.12, 90.00
R / Rfree (%)	19 / 25

The binding sites of Zinc atom in the Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site (pdb code 4p8q). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site, PDB code: 4p8q:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1101 b:53.2 occ:0.80 OE1 A:GLU487 1.9 30.7 1.0 NE2 A:HIS464 1.9 53.5 1.0 NE2 A:HIS468 2.2 45.8 1.0 O A:UNL1112 2.4 53.4 0.8 OE2 A:GLU487 2.4 68.7 1.0 CD A:GLU487 2.5 67.0 1.0 CD2 A:HIS464 2.9 53.8 1.0 N A:UNL1112 2.9 59.0 0.8 CE1 A:HIS464 3.0 53.4 1.0 CD2 A:HIS468 3.0 46.3 1.0 C A:UNL1112 3.1 82.5 0.8 CE1 A:HIS468 3.3 45.5 1.0 CA A:UNL1112 3.6 80.1 0.8 OE1 A:GLU431 3.9 67.9 1.0 CG A:GLU487 4.0 51.0 1.0 CG A:HIS464 4.0 52.7 1.0 ND1 A:HIS464 4.1 54.4 1.0 CG A:HIS468 4.2 45.2 1.0 ND1 A:HIS468 4.3 46.5 1.0 OE2 A:GLU465 4.4 0.7 1.0 CB A:ALA490 4.6 45.4 1.0 OE1 A:GLU465 4.7 0.1 1.0 CB A:UNL1112 4.7 80.8 0.8 CB A:GLU487 4.8 42.8 1.0 CD A:GLU431 4.8 73.3 1.0 CA A:GLU487 4.8 42.0 1.0 CD A:GLU465 4.9 0.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1101 b:54.3 occ:0.80 OE1 B:GLU487 1.9 33.4 1.0 NE2 B:HIS464 2.1 55.8 1.0 NE2 B:HIS468 2.1 65.2 1.0 O B:UNL1113 2.1 57.5 0.8 C B:UNL1113 2.2 85.7 0.8 CD B:GLU487 2.6 76.5 1.0 OE2 B:GLU487 2.7 82.2 1.0 CD2 B:HIS464 3.0 56.2 1.0 CD2 B:HIS468 3.0 65.3 1.0 CE1 B:HIS468 3.1 65.0 1.0 CE1 B:HIS464 3.1 55.6 1.0 CA B:UNL1113 3.2 65.3 0.8 N B:UNL1113 3.3 36.8 0.8 OE2 B:GLU431 3.9 95.2 1.0 CB B:UNL1113 4.0 66.2 0.8 CG B:GLU487 4.1 60.3 1.0 CG B:HIS468 4.1 63.5 1.0 CG B:HIS464 4.1 55.2 1.0 ND1 B:HIS468 4.1 65.5 1.0 ND1 B:HIS464 4.2 56.7 1.0 OE1 B:GLU465 4.3 0.1 1.0 OE2 B:GLU465 4.4 84.0 1.0 CD B:GLU431 4.5 91.4 1.0 CD B:GLU465 4.7 94.7 1.0 OE1 B:GLU431 4.7 73.6 1.0 CB B:GLU487 4.8 52.3 1.0 CA B:GLU487 4.9 51.4 1.0 CB B:ALA490 4.9 60.8 1.0

S.J.Hermans, D.B.Ascher, N.C.Hancock, J.K.Holien, B.J.Michell, S.Y.Chai, C.J.Morton, M.W.Parker. Crystal Structure of Human Insulin-Regulated Aminopeptidase with Specificity For Cyclic Peptides. Protein Sci. 2014.
ISSN: ESSN 1469-896X
PubMed: 25408552
DOI: 10.1002/PRO.2604