Zinc in PDB 4oif: 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G.

All present enzymatic activity of 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G.:
3.2.1.23;

The structure of 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G., PDB code: 4oif was solved by H.V.Solomon, O.Tabachnikov, H.Feinberg, Y.Shoham, G.Shoham, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.86 / 2.45
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	71.840, 181.350, 196.570, 90.00, 90.00, 90.00
R / Rfree (%)	13.3 / 20.4

The binding sites of Zinc atom in the 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G. (pdb code 4oif). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G., PDB code: 4oif:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:13.0 occ:1.00 SG A:CYS169 2.2 9.7 1.0 SG A:CYS166 2.4 9.7 1.0 SG A:CYS164 2.4 9.5 1.0 SG A:CYS124 2.4 12.6 1.0 CB A:CYS164 3.2 7.7 1.0 CB A:CYS124 3.4 11.2 1.0 CB A:CYS169 3.4 10.5 1.0 CB A:CYS166 3.5 8.8 1.0 N A:CYS166 3.9 8.4 1.0 N A:CYS169 4.0 7.8 1.0 O A:HOH1039 4.1 9.9 1.0 CA A:CYS124 4.1 7.8 1.0 CA A:CYS166 4.3 7.1 1.0 C A:CYS164 4.3 12.7 1.0 CA A:CYS169 4.3 9.4 1.0 CA A:CYS164 4.4 12.8 1.0 CB A:THR126 4.5 19.3 1.0 O A:CYS164 4.5 7.6 1.0 N A:HIS165 4.6 11.5 1.0 N A:THR126 4.6 12.4 1.0 C A:CYS124 4.7 14.1 1.0 N A:PHE125 4.7 17.1 1.0 CB A:LEU168 4.7 11.6 1.0 OG1 A:THR126 4.7 16.3 1.0 C A:CYS166 4.8 10.8 1.0 O A:CYS166 4.8 8.6 1.0

Zinc binding site 2 out of 3 in the 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G. within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn701 b:12.2 occ:1.00 SG B:CYS169 2.2 4.6 1.0 SG B:CYS164 2.3 10.1 1.0 SG B:CYS124 2.4 7.2 1.0 SG B:CYS166 2.4 7.3 1.0 CB B:CYS164 3.0 2.9 1.0 CB B:CYS124 3.4 7.7 1.0 CB B:CYS169 3.5 8.4 1.0 CB B:CYS166 3.5 7.0 1.0 N B:CYS166 3.9 8.1 1.0 N B:CYS169 4.1 7.6 1.0 CA B:CYS124 4.1 5.6 1.0 CA B:CYS166 4.2 8.9 1.0 CA B:CYS164 4.2 10.2 1.0 O B:HOH973 4.3 10.0 1.0 C B:CYS164 4.3 9.0 1.0 CA B:CYS169 4.3 5.7 1.0 CB B:THR126 4.4 9.4 1.0 N B:HIS165 4.6 7.1 1.0 O B:CYS164 4.6 7.0 1.0 C B:CYS124 4.6 8.3 1.0 N B:THR126 4.7 5.0 1.0 OG1 B:THR126 4.7 9.0 1.0 O B:CYS166 4.7 9.0 1.0 C B:CYS166 4.7 8.7 1.0 N B:PHE125 4.7 11.1 1.0 CE1 B:PHE125 4.8 20.2 1.0 CB B:LEU168 4.9 8.5 1.0 CD1 B:PHE125 4.9 9.9 1.0

Zinc binding site 3 out of 3 in the 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of 3D Structure of GAN42B, A GH42 Beta-Galactosidase From G. within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn701 b:16.3 occ:1.00 SG C:CYS164 2.3 14.2 1.0 SG C:CYS169 2.3 9.0 1.0 SG C:CYS166 2.3 11.3 1.0 SG C:CYS124 2.4 11.5 1.0 CB C:CYS164 3.3 11.8 1.0 CB C:CYS124 3.3 7.2 1.0 CB C:CYS166 3.4 8.9 1.0 CB C:CYS169 3.5 8.4 1.0 N C:CYS166 3.9 7.8 1.0 N C:CYS169 4.0 8.9 1.0 O C:HOH1201 4.1 4.2 1.0 CA C:CYS124 4.1 7.6 1.0 CA C:CYS166 4.2 8.2 1.0 CA C:CYS169 4.4 11.5 1.0 C C:CYS164 4.4 12.0 1.0 CA C:CYS164 4.5 10.5 1.0 CB C:LEU168 4.6 14.1 1.0 N C:HIS165 4.6 9.0 1.0 CB C:THR126 4.6 18.0 1.0 O C:CYS166 4.7 8.7 1.0 C C:CYS166 4.7 7.8 1.0 C C:CYS124 4.7 6.8 1.0 O C:CYS164 4.8 9.2 1.0 N C:PHE125 4.8 10.3 1.0 OG1 C:THR126 4.8 14.5 1.0 N C:THR126 4.8 12.4 1.0

H.V.Solomon, O.Tabachnikov, H.Feinberg, L.Govada, N.E.Chayen, Y.Shoham, G.Shoham. Crystallization and Preliminary Crystallographic Analysis of Ganb, A GH42 Intracellular Beta-Galactosidase From Geobacillus Stearothermophilus. Acta Crystallogr.,Sect.F V. 69 1114 2013.
ISSN: ISSN 1744-3091
PubMed: 24100561
DOI: 10.1107/S1744309113023609