Atomistry » Zinc » PDB 4ggk-4gsz » 4gk8
Atomistry »
  Zinc »
    PDB 4ggk-4gsz »
      4gk8 »

Zinc in PDB 4gk8: Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate

Enzymatic activity of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate

All present enzymatic activity of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate:
3.1.3.15;

Protein crystallography data

The structure of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate, PDB code: 4gk8 was solved by A.A.Fedorov, E.V.Fedorov, S.Ghodge, F.M.Raushel, S.C.Almo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.31 / 1.93
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 85.860, 86.622, 45.093, 90.00, 90.00, 90.00
R / Rfree (%) 16.2 / 19.6

Other elements in 4gk8:

The structure of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate also contains other interesting chemical elements:

Arsenic (As) 1 atom
Chlorine (Cl) 3 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate (pdb code 4gk8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate, PDB code: 4gk8:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 4gk8

Go back to Zinc Binding Sites List in 4gk8
Zinc binding site 1 out of 4 in the Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn301

b:15.9
occ:0.88
 O2 A:GK8305 1.9 21.9 0.8
OE2 A:GLU81 2.0 17.1 1.0
O A:HOH575 2.1 23.9 1.0
NE2 A:HIS109 2.1 19.3 1.0
NE2 A:HIS154 2.1 17.5 1.0
CE1 A:HIS109 3.0 18.4 1.0
CD A:GLU81 3.0 19.0 1.0
CD2 A:HIS154 3.1 15.0 1.0
CD2 A:HIS109 3.1 17.7 1.0
CE1 A:HIS154 3.2 19.0 1.0
AS A:GK8305 3.2 24.1 0.8
OE1 A:GLU81 3.3 16.3 1.0
ZN A:ZN303 3.7 17.4 0.8
O3 A:GK8305 3.7 21.4 0.8
OG A:SER107 3.9 19.9 1.0
CE1 A:TYR157 4.0 20.6 1.0
ND1 A:HIS109 4.1 17.2 1.0
O1 A:GK8305 4.2 21.2 0.8
CB A:SER107 4.2 15.2 1.0
CG A:HIS109 4.2 17.4 1.0
CG A:HIS154 4.2 15.4 1.0
ND1 A:HIS154 4.2 16.1 1.0
CG A:GLU81 4.3 18.2 1.0
CE1 A:HIS42 4.3 16.7 1.0
CD1 A:TYR157 4.3 18.9 1.0
N A:GK8305 4.4 21.1 0.8
CE1 A:HIS9 4.5 16.0 1.0
OD2 A:ASP228 4.5 17.9 1.0
NE2 A:HIS9 4.6 16.5 1.0
O4 A:GK8305 4.6 19.2 0.8
CZ A:TYR157 4.8 22.3 1.0

Zinc binding site 2 out of 4 in 4gk8

Go back to Zinc Binding Sites List in 4gk8
Zinc binding site 2 out of 4 in the Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn302

b:19.9
occ:1.00
 NE2 A:HIS42 2.1 16.8 1.0
OD2 A:ASP17 2.1 16.9 1.0
NE2 A:HIS230 2.1 18.5 1.0
O4 A:GK8305 2.2 19.2 0.8
O3 A:GK8305 2.7 21.4 0.8
CG A:ASP17 2.8 16.6 1.0
OD1 A:ASP17 2.9 16.2 1.0
AS A:GK8305 3.1 24.1 0.8
CE1 A:HIS42 3.1 16.7 1.0
CD2 A:HIS42 3.1 14.5 1.0
CE1 A:HIS230 3.1 21.7 1.0
CD2 A:HIS230 3.1 16.8 1.0
C A:GK8305 3.3 28.7 0.8
CA A:GK8305 3.8 24.8 0.8
NE2 A:HIS11 4.1 15.7 1.0
ND1 A:HIS42 4.2 16.4 1.0
ND1 A:HIS230 4.2 15.2 1.0
CG A:HIS42 4.2 16.0 1.0
CG A:HIS230 4.2 17.6 1.0
O1 A:GK8305 4.2 21.2 0.8
CE1 A:HIS11 4.2 18.3 1.0
O2 A:GK8305 4.2 21.9 0.8
CD2 A:HIS11 4.3 15.0 1.0
O A:HOH557 4.3 39.2 1.0
CB A:ASP17 4.3 17.6 1.0
ZN A:ZN303 4.4 17.4 0.8
ND1 A:HIS11 4.4 16.1 1.0
N A:GK8305 4.5 21.1 0.8
CG A:HIS11 4.5 15.9 1.0
CD2 A:GK8305 4.8 27.6 0.8
CE1 A:PHE52 4.9 18.7 1.0

Zinc binding site 3 out of 4 in 4gk8

Go back to Zinc Binding Sites List in 4gk8
Zinc binding site 3 out of 4 in the Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn303

b:17.4
occ:0.84
 O3 A:GK8305 1.8 21.4 0.8
O A:HOH575 2.1 23.9 1.0
NE2 A:HIS9 2.1 16.5 1.0
NE2 A:HIS11 2.2 15.7 1.0
OE1 A:GLU81 2.2 16.3 1.0
OD1 A:ASP228 2.3 19.9 1.0
CE1 A:HIS11 3.0 18.3 1.0
CE1 A:HIS9 3.1 16.0 1.0
CD2 A:HIS9 3.1 16.9 1.0
CD A:GLU81 3.1 19.0 1.0
CG A:ASP228 3.2 20.7 1.0
CD2 A:HIS11 3.2 15.0 1.0
AS A:GK8305 3.2 24.1 0.8
OD2 A:ASP228 3.4 17.9 1.0
OE2 A:GLU81 3.5 17.1 1.0
ZN A:ZN301 3.7 15.9 0.9
O2 A:GK8305 3.9 21.9 0.8
CE1 A:HIS42 3.9 16.7 1.0
O1 A:GK8305 4.1 21.2 0.8
ND1 A:HIS9 4.2 17.1 1.0
ND1 A:HIS11 4.2 16.1 1.0
CE1 A:HIS230 4.2 21.7 1.0
CG A:HIS9 4.2 14.4 1.0
NE2 A:HIS42 4.2 16.8 1.0
CG A:HIS11 4.3 15.9 1.0
ZN A:ZN302 4.4 19.9 1.0
CG A:GLU81 4.4 18.2 1.0
CB A:ASP228 4.5 18.7 1.0
NE2 A:HIS230 4.6 18.5 1.0
O4 A:GK8305 4.6 19.2 0.8
CB A:GLU81 4.7 17.5 1.0
ND1 A:HIS42 4.8 16.4 1.0
CA A:ASP228 4.8 16.1 1.0

Zinc binding site 4 out of 4 in 4gk8

Go back to Zinc Binding Sites List in 4gk8
Zinc binding site 4 out of 4 in the Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Histidinol Phosphate Phosphatase (Hisk) From Lactococcus Lactis Subsp. Lactis IL1403 Complexed with Zn and L- Histidinol Arsenate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn304

b:21.4
occ:1.00
 N3 A:IMD309 2.0 21.6 1.0
NE2 A:HIS32 2.0 20.7 1.0
NE2 A:HIS240 2.0 22.4 1.0
CL A:CL306 2.2 24.2 1.0
C2 A:IMD309 2.9 23.7 1.0
CD2 A:HIS240 2.9 21.0 1.0
CD2 A:HIS32 3.0 16.2 1.0
CE1 A:HIS32 3.0 18.6 1.0
CE1 A:HIS240 3.1 19.2 1.0
C4 A:IMD309 3.1 21.8 1.0
N1 A:IMD309 4.1 28.4 1.0
CG A:HIS32 4.1 18.0 1.0
ND1 A:HIS32 4.1 17.4 1.0
CG A:HIS240 4.1 20.0 1.0
ND1 A:HIS240 4.2 19.4 1.0
C5 A:IMD309 4.2 23.2 1.0
CE3 A:TRP236 4.3 20.7 1.0
CZ3 A:TRP236 4.8 19.8 1.0
CD2 A:TRP236 4.9 17.9 1.0

Reference:

S.V.Ghodge, A.A.Fedorov, E.V.Fedorov, B.Hillerich, R.Seidel, S.C.Almo, F.M.Raushel. Structural and Mechanistic Characterization of L-Histidinol Phosphate Phosphatase From the Polymerase and Histidinol Phosphatase Family of Proteins. Biochemistry V. 52 1101 2013.
ISSN: ISSN 0006-2960
PubMed: 23327428
DOI: 10.1021/BI301496P
Page generated: Sat Oct 26 23:19:31 2024

Last articles

Mg in 5IPL
Mg in 5IPN
Mg in 5IPM
Mg in 5IN8
Mg in 5IOO
Mg in 5IP9
Mg in 5INM
Mg in 5IP7
Mg in 5INL
Mg in 5INK
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy