Atomistry » Zinc » PDB 3boc-3c4u » 3bup
Atomistry »
  Zinc »
    PDB 3boc-3c4u »
      3bup »

Zinc in PDB 3bup: Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose

Enzymatic activity of Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose

All present enzymatic activity of Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose:
3.2.1.114;

Protein crystallography data

The structure of Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose, PDB code: 3bup was solved by D.A.Kuntz, D.R.Rose, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.79 / 2.03
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 68.670, 109.486, 138.584, 90.00, 90.00, 90.00
R / Rfree (%) 15.2 / 19.5

Zinc Binding Sites:

The binding sites of Zinc atom in the Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose (pdb code 3bup). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose, PDB code: 3bup:

Zinc binding site 1 out of 1 in 3bup

Go back to Zinc Binding Sites List in 3bup
Zinc binding site 1 out of 1 in the Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Golgi Alpha-Mannosidase II D341N Acid-Base Catalyst Mutant with Bound Mannose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1048

b:18.4
occ:1.00
OD1 A:ASP92 2.0 16.2 1.0
NE2 A:HIS90 2.1 13.8 1.0
NE2 A:HIS471 2.1 12.3 1.0
OD1 A:ASP204 2.3 14.2 1.0
O3 A:MAN1047 2.4 23.8 1.0
O2 A:MAN1047 2.6 23.4 1.0
CD2 A:HIS90 3.0 13.2 1.0
CG A:ASP92 3.0 16.6 1.0
C2 A:MAN1047 3.0 25.1 1.0
CD2 A:HIS471 3.0 13.1 1.0
C3 A:MAN1047 3.1 23.4 1.0
CE1 A:HIS90 3.1 13.2 1.0
CE1 A:HIS471 3.2 13.2 1.0
OD2 A:ASP92 3.2 18.4 1.0
CG A:ASP204 3.3 15.5 1.0
CB A:ASP204 3.6 13.6 1.0
C1 A:MAN1047 4.0 25.7 1.0
OD2 A:ASP472 4.1 14.4 1.0
C4 A:MAN1047 4.1 22.2 1.0
CG A:HIS90 4.2 13.8 1.0
ND1 A:HIS90 4.2 13.7 1.0
CG A:HIS471 4.2 13.7 1.0
ND1 A:HIS471 4.3 12.8 1.0
CE1 A:HIS470 4.3 13.0 1.0
CB A:ASP92 4.4 15.1 1.0
OD2 A:ASP204 4.4 14.3 1.0
O A:HOH2106 4.6 17.7 1.0
O5 A:MAN1047 4.6 24.6 1.0
OH A:TYR269 4.8 15.4 1.0
NE2 A:HIS470 4.9 13.9 1.0
CA A:ASP92 4.9 15.5 1.0

Reference:

W.Zhong, D.A.Kuntz, B.Ember, H.Singh, K.W.Moremen, D.R.Rose, G.J.Boons. Probing the Substrate Specificity of Golgi Alpha-Mannosidase II By Use of Synthetic Oligosaccharides and A Catalytic Nucleophile Mutant. J.Am.Chem.Soc. V. 130 8975 2008.
ISSN: ISSN 0002-7863
PubMed: 18558690
DOI: 10.1021/JA711248Y
Page generated: Thu Oct 24 11:37:42 2024

Last articles

Zn in 9MJ5
Zn in 9HNW
Zn in 9G0L
Zn in 9FNE
Zn in 9DZN
Zn in 9E0I
Zn in 9D32
Zn in 9DAK
Zn in 8ZXC
Zn in 8ZUF
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy