Zinc in PDB 2wxu: Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I

All present enzymatic activity of Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I:
3.1.4.3;

The structure of Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I, PDB code: 2wxu was solved by S.G.Vachieri, C.E.Naylor, A.K.Basak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.64 / 1.80
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	59.861, 173.643, 78.564, 90.00, 90.00, 90.00
R / Rfree (%)	17.5 / 21.6

The structure of Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I also contains other interesting chemical elements:

Cadmium	(Cd)	7 atoms
Calcium	(Ca)	5 atoms

The binding sites of Zinc atom in the Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I (pdb code 2wxu). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I, PDB code: 2wxu:

Zinc binding site 1 out of 1 in the Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Clostridium Perfringens Alpha-Toxin Strain NCTC8237 Mutant T74I within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1375 b:19.3 occ:1.00 O A:HOH2402 1.9 16.0 1.0 NE2 A:HIS11 2.0 18.6 1.0 OD1 A:ASP130 2.1 16.1 1.0 N A:TRP1 2.2 19.9 1.0 O A:TRP1 2.3 16.4 1.0 C A:TRP1 2.9 20.5 1.0 CA A:TRP1 3.0 16.5 1.0 CD2 A:HIS11 3.0 17.4 1.0 CE1 A:HIS11 3.0 17.8 1.0 O A:HOH2190 3.2 28.5 1.0 CG A:ASP130 3.2 19.5 1.0 OD2 A:ASP130 3.6 16.1 1.0 CB A:TRP1 3.6 18.4 1.0 CD A:CD1372 3.6 19.3 1.0 CE1 A:HIS126 3.9 18.2 1.0 NE2 A:HIS126 4.0 15.9 1.0 OD2 A:ASP56 4.1 20.9 1.0 ND1 A:HIS11 4.1 16.5 1.0 CG A:HIS11 4.2 14.1 1.0 N A:ASP2 4.3 17.5 1.0 CG A:TRP1 4.4 18.4 1.0 CB A:ASP130 4.5 19.8 1.0 ND1 A:HIS126 4.7 14.2 1.0 O A:HOH2107 4.7 22.2 1.0 CD1 A:TRP1 4.7 14.7 1.0 CA A:ASP130 4.7 17.7 1.0 OE2 A:GLU152 4.8 23.9 1.0 CD A:CD1373 4.8 23.7 1.0 CD2 A:HIS126 4.9 12.1 1.0 OD1 A:ASP56 4.9 18.8 1.0 CG A:ASP56 4.9 20.0 1.0 O A:HOH2006 4.9 33.1 1.0 N A:ASP130 5.0 16.2 1.0 NE2 A:HIS136 5.0 17.3 1.0

S.G.Vachieri, G.C.Clark, A.Alape-Giron, M.Flores-Diaz, N.Justin, C.E.Naylor, R.W.Titball, A.K.Basak. Comparison of A Nontoxic Variant of Clostridium Perfringens [Alpha]-Toxin with the Toxic Wild-Type Strain Acta Crystallogr.,Sect.D V. 66 1067 2010.
ISSN: ISSN 0907-4449
PubMed: 20944240
DOI: 10.1107/S090744491003369X