Zinc in PDB 2qf7: Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli

All present enzymatic activity of Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli:
6.4.1.1;

The structure of Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli, PDB code: 2qf7 was solved by M.St Maurice, K.H.Surinya, I.Rayment, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	131.31 / 2.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	234.729, 93.258, 137.222, 90.00, 107.33, 90.00
R / Rfree (%)	17.8 / 22.3

The structure of Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli also contains other interesting chemical elements:

Magnesium	(Mg)	6 atoms
Chlorine	(Cl)	4 atoms

The binding sites of Zinc atom in the Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli (pdb code 2qf7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli, PDB code: 2qf7:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1157 b:15.8 occ:1.00 NE2 A:HIS747 2.1 26.4 1.0 NE2 A:HIS749 2.1 21.9 1.0 OQ1 A:KCX718 2.2 23.9 1.0 OD2 A:ASP549 2.2 20.4 1.0 O A:HOH1476 2.3 13.2 1.0 OQ2 A:KCX718 2.3 21.6 1.0 CX A:KCX718 2.5 27.7 1.0 CE1 A:HIS749 2.9 23.6 1.0 CE1 A:HIS747 3.0 26.7 1.0 CD2 A:HIS747 3.1 25.5 1.0 CG A:ASP549 3.1 23.6 1.0 CD2 A:HIS749 3.3 23.0 1.0 OD1 A:ASP549 3.4 25.2 1.0 NZ A:KCX718 3.9 24.3 1.0 O A:HOH1257 4.0 15.5 1.0 NE2 A:GLN783 4.0 23.7 1.0 ND1 A:HIS749 4.1 21.9 1.0 ND1 A:HIS747 4.2 24.4 1.0 O A:HOH1210 4.2 17.1 1.0 CG A:HIS747 4.2 24.9 1.0 NH1 A:ARG548 4.3 22.2 1.0 CG A:HIS749 4.3 22.2 1.0 CB A:ASP549 4.5 20.4 1.0 O A:HOH1242 4.6 20.1 1.0 CA A:MET720 4.9 23.5 1.0 O A:HOH1477 5.0 21.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1155 b:16.5 occ:1.00 OD2 B:ASP549 2.1 20.4 1.0 NE2 B:HIS747 2.1 20.6 1.0 O B:HOH1335 2.2 10.3 1.0 NE2 B:HIS749 2.2 24.7 1.0 OQ1 B:KCX718 2.2 28.6 1.0 OQ2 B:KCX718 2.3 21.1 1.0 CX B:KCX718 2.5 30.0 1.0 CE1 B:HIS749 3.0 22.1 1.0 CE1 B:HIS747 3.1 22.8 1.0 CG B:ASP549 3.1 23.7 1.0 CD2 B:HIS747 3.2 20.1 1.0 CD2 B:HIS749 3.3 20.2 1.0 OD1 B:ASP549 3.4 24.0 1.0 NZ B:KCX718 3.9 26.6 1.0 NE2 B:GLN783 3.9 23.2 1.0 O B:HOH1171 4.0 14.5 1.0 ND1 B:HIS749 4.2 20.8 1.0 ND1 B:HIS747 4.2 18.4 1.0 O B:HOH1180 4.3 15.4 1.0 CG B:HIS747 4.3 21.9 1.0 NH1 B:ARG548 4.3 21.7 1.0 CB B:ASP549 4.3 23.8 1.0 CG B:HIS749 4.3 21.4 1.0 CA B:MET720 4.8 22.6 1.0 CD B:GLN783 5.0 23.0 1.0

M.St Maurice, L.Reinhardt, K.H.Surinya, P.V.Attwood, J.C.Wallace, W.W.Cleland, I.Rayment. Domain Architecture of Pyruvate Carboxylase, A Biotin-Dependent Multifunctional Enzyme Science V. 317 1076 2007.
ISSN: ISSN 0036-8075
PubMed: 17717183
DOI: 10.1126/SCIENCE.1144504