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Zinc in PDB 2iu2: Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions

Enzymatic activity of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions

All present enzymatic activity of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions:
1.16.3.1;

Protein crystallography data

The structure of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions, PDB code: 2iu2 was solved by L.Toussaint, R.R.Crichton, J.P.Declercq, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 104.26 / 1.80
Space group F 4 3 2
Cell size a, b, c (Å), α, β, γ (°) 181.050, 181.050, 181.050, 90.00, 90.00, 90.00
R / Rfree (%) 19.3 / 21.9

Zinc Binding Sites:

The binding sites of Zinc atom in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions (pdb code 2iu2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions, PDB code: 2iu2:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in 2iu2

Go back to Zinc Binding Sites List in 2iu2
Zinc binding site 1 out of 6 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn210

b:24.7
occ:0.50
 OE1 A:GLN86 2.2 26.9 1.0
CD A:GLN86 3.3 22.7 1.0
NE2 A:GLN86 3.7 24.2 1.0
O A:HOH2090 4.1 22.3 1.0
CG A:GLN86 4.7 19.6 1.0
O A:HOH2089 4.9 53.2 1.0

Zinc binding site 2 out of 6 in 2iu2

Go back to Zinc Binding Sites List in 2iu2
Zinc binding site 2 out of 6 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn211

b:46.9
occ:0.33
 OD1 A:ASP131 3.6 28.7 1.0
OG1 A:THR135 4.0 26.7 1.0
OE1 A:GLU134 4.5 34.3 1.0
CG A:ASP131 4.7 27.7 1.0

Zinc binding site 3 out of 6 in 2iu2

Go back to Zinc Binding Sites List in 2iu2
Zinc binding site 3 out of 6 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn214

b:35.3
occ:0.25
 O A:HOH2150 2.2 12.9 0.2
NE2 A:HIS173 2.3 30.6 1.0
CE1 A:HIS173 2.9 32.6 1.0
CD2 A:HIS173 3.5 27.9 1.0
ND1 A:HIS173 4.2 28.8 1.0
CG A:HIS173 4.5 24.4 1.0
CD2 A:LEU169 4.9 17.2 1.0

Zinc binding site 4 out of 6 in 2iu2

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Zinc binding site 4 out of 6 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn215

b:84.4
occ:1.00
 CE1 A:HIS60 2.4 30.3 1.0
NE2 A:HIS60 2.4 29.4 1.0
ND1 A:HIS60 3.6 30.4 1.0
CD2 A:HIS60 3.7 28.8 1.0
CG A:HIS60 4.3 25.1 1.0
CD2 A:LEU56 4.6 19.1 1.0
CD1 A:LEU56 4.7 20.4 1.0
CG A:LEU56 4.8 18.0 1.0

Zinc binding site 5 out of 6 in 2iu2

Go back to Zinc Binding Sites List in 2iu2
Zinc binding site 5 out of 6 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn219

b:38.5
occ:1.00
 NE2 A:HIS65 2.1 28.1 1.0
OE2 A:GLU61 2.2 35.8 1.0
O A:HOH2151 2.3 33.8 1.0
CD2 A:HIS65 3.1 24.9 1.0
CD A:GLU61 3.1 34.6 1.0
CE1 A:HIS65 3.2 27.8 1.0
CG A:GLU61 3.3 27.0 1.0
CG A:HIS65 4.2 22.2 1.0
ND1 A:HIS65 4.2 24.5 1.0
OE1 A:GLU61 4.3 38.1 1.0
CG A:GLU140 4.5 29.7 1.0
OE1 A:GLU140 4.6 42.2 1.0
O A:HOH2035 4.7 39.8 1.0
CB A:GLU140 4.7 24.1 1.0
CB A:GLU61 4.8 21.1 1.0
O A:GLU61 4.8 18.5 1.0
CD A:GLU140 4.9 38.6 1.0

Zinc binding site 6 out of 6 in 2iu2

Go back to Zinc Binding Sites List in 2iu2
Zinc binding site 6 out of 6 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn220

b:32.7
occ:0.33
 O A:HOH2152 2.0 38.3 0.3
OE1 A:GLU134 2.3 34.3 1.0
CD A:GLU134 2.8 27.5 1.0
OE2 A:GLU134 3.0 35.6 1.0
CG A:GLU134 4.1 28.1 1.0
CB A:GLU134 4.8 24.3 1.0

Reference:

L.Toussaint, L.Bertrand, L.Hue, R.R.Crichton, J.P.Declercq. High-Resolution X-Ray Structures of Human Apoferritin H-Chain Mutants Correlated with Their Activity and Metal-Binding Sites. J.Mol.Biol. V. 365 440 2007.
ISSN: ISSN 0022-2836
PubMed: 17070541
DOI: 10.1016/J.JMB.2006.10.010
Page generated: Thu Oct 17 00:59:40 2024

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