Zinc in PDB 2imz: Crystal Structure of Mtu Reca Intein Splicing Domain

The structure of Crystal Structure of Mtu Reca Intein Splicing Domain, PDB code: 2imz was solved by P.Van Roey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.76 / 1.70
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	82.430, 93.630, 40.140, 90.00, 90.00, 90.00
R / Rfree (%)	18.1 / 21

The binding sites of Zinc atom in the Crystal Structure of Mtu Reca Intein Splicing Domain (pdb code 2imz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Mtu Reca Intein Splicing Domain, PDB code: 2imz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Mtu Reca Intein Splicing Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Mtu Reca Intein Splicing Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:15.4 occ:1.00 ND1 A:HIS429 2.0 15.1 1.0 NE2 B:HIS439 2.0 17.1 1.0 N1 A:SNN440 2.1 14.2 1.0 OE1 A:GLU424 2.2 18.2 1.0 CE1 A:HIS429 2.8 14.9 1.0 OE2 A:GLU424 2.9 19.4 1.0 CD A:GLU424 2.9 14.2 1.0 C5 A:SNN440 2.9 14.6 1.0 CE1 B:HIS439 3.0 19.6 1.0 CD2 B:HIS439 3.0 17.2 1.0 O5 A:SNN440 3.0 14.4 1.0 CG A:HIS429 3.1 14.9 1.0 C2 A:SNN440 3.1 18.6 1.0 O2 A:SNN440 3.4 23.7 1.0 CB A:HIS429 3.6 15.3 1.0 O A:HOH521 3.9 18.5 1.0 NE2 A:HIS429 4.0 16.3 1.0 CA A:HIS429 4.0 12.9 1.0 ND1 B:HIS439 4.1 18.2 1.0 CD2 A:HIS429 4.1 17.7 1.0 CG B:HIS439 4.1 13.6 1.0 C4 A:SNN440 4.3 14.9 1.0 C3 A:SNN440 4.3 15.3 1.0 CG A:GLU424 4.4 17.8 1.0 NZ B:LYS74 4.4 28.4 1.0 O A:HIS429 4.6 12.1 1.0 C A:HIS429 4.8 13.0 1.0 O A:HOH511 4.9 29.0 1.0 CE B:LYS74 5.0 24.6 1.0 CA A:GLU424 5.0 12.8 1.0 CB A:GLU424 5.0 14.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Mtu Reca Intein Splicing Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Mtu Reca Intein Splicing Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn502 b:13.8 occ:1.00 OE1 B:GLU424 2.0 13.9 1.0 NE2 A:HIS439 2.0 14.9 1.0 ND1 B:HIS429 2.0 11.1 1.0 N1 B:SNN440 2.1 16.5 1.0 CD B:GLU424 2.8 14.7 1.0 C5 B:SNN440 2.8 16.4 1.0 OE2 B:GLU424 2.9 19.0 1.0 CE1 B:HIS429 2.9 13.3 1.0 O5 B:SNN440 2.9 12.6 1.0 CE1 A:HIS439 3.0 19.2 1.0 CD2 A:HIS439 3.0 16.9 1.0 CG B:HIS429 3.2 12.2 1.0 C2 B:SNN440 3.2 17.2 1.0 O2 B:SNN440 3.6 22.2 1.0 CB B:HIS429 3.6 11.3 1.0 O B:HOH512 3.9 15.4 1.0 NE2 B:HIS429 4.1 9.8 1.0 ND1 A:HIS439 4.1 16.9 1.0 CG A:HIS439 4.2 14.8 1.0 CA B:HIS429 4.2 11.0 1.0 CD2 B:HIS429 4.2 10.0 1.0 CG B:GLU424 4.2 15.8 1.0 C4 B:SNN440 4.2 15.5 1.0 C3 B:SNN440 4.4 15.9 1.0 O B:HOH596 4.4 36.0 1.0 O A:HOH651 4.4 49.2 1.0 O B:HIS429 4.7 12.0 1.0 CA B:GLU424 4.8 13.7 1.0 CB B:GLU424 4.8 15.2 1.0 C B:HIS429 4.9 11.3 1.0

P.Van Roey, B.Pereira, Z.Li, K.Hiraga, M.Belfort, V.Derbyshire. Crystallographic and Mutational Studies of Mycobacterium Tuberculosis Reca Mini-Inteins Suggest A Pivotal Role For A Highly Conserved Aspartate Residue. J.Mol.Biol. V. 367 162 2007.
ISSN: ISSN 0022-2836
PubMed: 17254599
DOI: 10.1016/J.JMB.2006.12.050