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Zinc in PDB 2f56: Barnase Cross-Linked with Glutaraldehyde Soaked in 6M Urea

Protein crystallography data

The structure of Barnase Cross-Linked with Glutaraldehyde Soaked in 6M Urea, PDB code: 2f56 was solved by T.Prange, M.Salem, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 10.00 / 1.96
Space group P 32
Cell size a, b, c (Å), α, β, γ (°) 59.280, 59.280, 81.550, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Zinc Binding Sites:

The binding sites of Zinc atom in the Barnase Cross-Linked with Glutaraldehyde Soaked in 6M Urea (pdb code 2f56). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Barnase Cross-Linked with Glutaraldehyde Soaked in 6M Urea, PDB code: 2f56:

Zinc binding site 1 out of 1 in 2f56

Go back to Zinc Binding Sites List in 2f56
Zinc binding site 1 out of 1 in the Barnase Cross-Linked with Glutaraldehyde Soaked in 6M Urea


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Barnase Cross-Linked with Glutaraldehyde Soaked in 6M Urea within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn300

b:46.0
occ:1.00
ND1 C:HIS16 2.6 19.6 1.0
CE1 C:HIS16 3.4 16.7 1.0
CG C:HIS16 3.6 24.3 1.0
CB C:HIS16 4.0 19.7 1.0
O A:LEU31 4.2 16.9 1.0
O C:TYR15 4.2 21.8 1.0
NE2 C:HIS16 4.6 17.4 1.0
C C:TYR15 4.6 14.7 1.0
O A:VAL43 4.7 21.6 1.0
CD2 C:HIS16 4.7 19.3 1.0
O C:HOH381 5.0 24.9 1.0

Reference:

M.Salem, Y.Mauguen, T.Prange. On the Edge of the Denaturation Process: Application of X-Ray Diffraction to Barnase and Lysozyme Cross-Linked Crystals with Denaturants in Molar Concentrations. Biochim.Biophys.Acta V.1764 903 2006.
ISSN: ISSN 0006-3002
PubMed: 16600702
DOI: 10.1016/J.BBAPAP.2006.02.009
Page generated: Wed Oct 16 23:38:41 2024

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