Zinc in PDB 2eu8: Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R)

All present enzymatic activity of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R):
2.7.4.3;

The structure of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R), PDB code: 2eu8 was solved by S.Chen, Y.Shamoo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.57 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	34.690, 75.140, 77.350, 90.00, 98.13, 90.00
R / Rfree (%)	18.7 / 22.4

The structure of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R) also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Calcium	(Ca)	1 atom

The binding sites of Zinc atom in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R) (pdb code 2eu8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R), PDB code: 2eu8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn220 b:13.0 occ:1.00 OD2 A:ASP153 2.2 7.5 1.0 SG A:CYS130 2.3 8.9 1.0 SG A:CYS150 2.4 8.7 1.0 SG A:CYS133 2.4 12.4 1.0 OD1 A:ASP153 2.8 9.5 1.0 CG A:ASP153 2.8 11.4 1.0 CB A:CYS130 3.1 9.3 1.0 CB A:CYS150 3.1 8.7 1.0 CB A:CYS133 3.3 13.7 1.0 N A:CYS133 3.6 13.7 1.0 CA A:CYS133 4.0 12.8 1.0 CB A:ASP153 4.2 11.1 1.0 CG2 A:THR135 4.3 13.3 1.0 N A:ASP153 4.4 12.6 1.0 CB A:VAL132 4.4 14.6 1.0 O A:HOH1323 4.4 29.4 1.0 CA A:CYS130 4.6 7.6 1.0 C A:VAL132 4.6 14.3 1.0 CA A:CYS150 4.6 10.9 1.0 CB A:LYS152 4.6 14.4 1.0 C A:CYS133 4.8 12.1 1.0 N A:GLY134 4.8 10.9 1.0 CA A:ASP153 4.9 10.5 1.0 CA A:VAL132 4.9 13.8 1.0 CG1 A:VAL132 4.9 14.1 1.0 N A:VAL132 4.9 11.4 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (Q199R) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn221 b:15.9 occ:1.00 OD2 B:ASP153 2.2 16.3 1.0 SG B:CYS150 2.4 13.3 1.0 SG B:CYS130 2.4 11.8 1.0 SG B:CYS133 2.4 14.0 1.0 CG B:ASP153 2.9 17.9 1.0 OD1 B:ASP153 2.9 14.6 1.0 CB B:CYS150 3.2 12.6 1.0 CB B:CYS130 3.2 13.7 1.0 CB B:CYS133 3.4 16.6 1.0 N B:CYS133 3.6 15.4 1.0 CG2 B:THR135 4.0 18.9 1.0 CA B:CYS133 4.1 15.4 1.0 CB B:ASP153 4.3 16.1 1.0 O B:HOH1313 4.3 25.9 1.0 CB B:VAL132 4.4 14.6 1.0 N B:ASP153 4.4 16.0 1.0 CB B:LYS152 4.5 17.5 1.0 C B:VAL132 4.6 16.0 1.0 CA B:CYS130 4.6 13.5 1.0 CA B:CYS150 4.6 13.8 1.0 CD B:LYS152 4.7 21.7 1.0 N B:GLY134 4.7 16.0 1.0 C B:CYS133 4.8 16.1 1.0 CA B:VAL132 4.9 15.9 1.0 N B:VAL132 4.9 15.1 1.0 CA B:ASP153 4.9 16.5 1.0 CG1 B:VAL132 5.0 16.6 1.0

R.Counago, S.Chen, Y.Shamoo. In Vivo Molecular Evolution Reveals Biophysical Origins of Organismal Fitness. Mol.Cell V. 22 441 2006.
ISSN: ISSN 1097-2765
PubMed: 16713575
DOI: 10.1016/J.MOLCEL.2006.04.012