Zinc in PDB 2b8o: Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex

All present enzymatic activity of Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex:
3.4.21.21;

The structure of Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex, PDB code: 2b8o was solved by S.P.Bajaj, A.E.Schmidt, K.Padmanabhan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.280, 81.110, 126.860, 90.00, 90.00, 90.00
R / Rfree (%)	22.5 / 28.5

The structure of Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Calcium	(Ca)	6 atoms
Chlorine	(Cl)	3 atoms
Sodium	(Na)	1 atom

The binding sites of Zinc atom in the Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex (pdb code 2b8o). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex, PDB code: 2b8o:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex within 5.0Å range: probe atom residue distance (Å) B Occ H:Zn512 b:44.9 occ:1.00 CE1 H:HIS76 2.9 17.4 1.0 O H:HOH578 3.4 33.1 1.0 O H:HOH562 3.5 43.7 1.0 OG H:SER82 3.7 10.8 1.0 ND1 H:HIS76 3.8 15.5 1.0 NE2 H:HIS76 3.9 11.5 1.0 CG1 H:VAL67 4.3 2.0 1.0 CG2 H:VAL67 4.4 2.0 1.0 OE1 H:GLU70 4.7 21.1 1.0 CB H:GLU80 4.9 11.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Glu-Gly-Arg-Chloromethyl Ketone-Factor Viia/Soluble Tissue Factor Complex within 5.0Å range: probe atom residue distance (Å) B Occ H:Zn513 b:57.1 occ:1.00 NZ H:LYS24 2.3 24.1 1.0 O H:HOH525 2.7 20.3 1.0 CG H:LYS24 2.9 15.6 1.0 O H:GLY69 3.0 13.5 1.0 CE H:LYS24 3.1 18.8 1.0 CD H:LYS24 3.3 14.8 1.0 ND1 H:HIS117 3.8 8.5 1.0 CB H:HIS71 3.9 15.7 1.0 CB H:ASP79 4.0 12.2 1.0 C H:GLY69 4.1 7.7 1.0 CB H:HIS117 4.1 6.3 1.0 OD2 H:ASP79 4.2 15.1 1.0 CG H:HIS117 4.2 6.6 1.0 OD1 H:ASP77 4.3 24.7 1.0 CB H:LYS24 4.3 12.6 1.0 CG H:ASP79 4.5 14.3 1.0 N H:HIS71 4.6 17.1 1.0 CE1 H:HIS117 4.7 10.0 1.0 N H:GLY25 4.7 14.2 1.0 CA H:HIS71 4.8 14.7 1.0 C H:GLU70 4.8 15.7 1.0 CA H:GLY69 4.8 7.0 1.0 CA H:LYS24 4.8 14.8 1.0 O H:GLU70 4.8 17.6 1.0 C H:LYS24 4.9 13.9 1.0 CG H:HIS71 4.9 15.9 1.0

S.P.Bajaj, A.E.Schmidt, K.Padmanabhan. Crystal Structure of Factor Viia/Soluble Tissue Factor Complexed with Glu-Gly-Arg-Chloromethyl Ketone To Be Published.