Zinc in PDB 258l: An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme

All present enzymatic activity of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme:
3.2.1.17;

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 258l was solved by J.W.Wray, W.A.Baase, G.J.Ostheimer, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.330, 61.330, 97.470, 90.00, 90.00, 120.00
R / Rfree (%)	17.3 / n/a

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme (pdb code 258l). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 258l:

Zinc binding site 1 out of 1 in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn500 b:25.6 occ:1.00 NE2 A:HIS21 1.9 20.8 1.0 OE2 A:GLU22 1.9 20.7 1.0 O A:HOH501 2.2 13.9 1.0 NE2 A:HIS142 2.3 18.6 1.0 CD2 A:HIS21 2.9 11.7 1.0 CE1 A:HIS21 2.9 22.5 1.0 CD A:GLU22 3.0 25.4 1.0 CD2 A:HIS142 3.1 16.7 1.0 O A:HOH256 3.3 45.8 1.0 CG A:GLU22 3.3 18.3 1.0 CE1 A:HIS142 3.4 16.5 1.0 CG A:HIS21 4.0 16.7 1.0 ND1 A:HIS21 4.0 29.3 1.0 NE2 A:GLN105 4.0 22.2 1.0 OE1 A:GLU22 4.1 25.5 1.0 CG A:HIS142 4.3 15.3 1.0 NH1 A:ARG137 4.3 16.8 1.0 ND1 A:HIS142 4.4 15.7 1.0 CB A:GLU22 4.8 19.7 1.0 O A:HOH172 4.8 20.8 1.0

J.W.Wray, W.A.Baase, G.J.Ostheimer, X.J.Zhang, B.W.Matthews. Use of A Non-Rigid Region in T4 Lysozyme to Design An Adaptable Metal-Binding Site. Protein Eng. V. 13 313 2000.
ISSN: ISSN 0269-2139
PubMed: 10835104
DOI: 10.1093/PROTEIN/13.5.313