Zinc in PDB 1znj: Insulin, Monoclinic Crystal Form

The structure of Insulin, Monoclinic Crystal Form, PDB code: 1znj was solved by M.G.W.Turkenburg, J.L.Whittingham, J.P.Turkenburg, G.G.Dodson, U.Derewenda, G.D.Smith, E.J.Dodson, Z.S.Derewenda, B.Xiao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	61.230, 61.650, 48.050, 90.00, 110.50, 90.00
R / Rfree (%)	17.8 / n/a

The structure of Insulin, Monoclinic Crystal Form also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Insulin, Monoclinic Crystal Form (pdb code 1znj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Insulin, Monoclinic Crystal Form, PDB code: 1znj:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Insulin, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Insulin, Monoclinic Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn31 b:18.0 occ:1.00 NE2 B:HIS10 1.8 7.6 1.0 NE2 J:HIS10 2.0 15.5 1.0 NE2 F:HIS10 2.2 21.8 1.0 CL B:CL32 2.3 21.2 1.0 CE1 J:HIS10 2.7 14.4 1.0 CE1 B:HIS10 2.8 12.0 1.0 CD2 B:HIS10 2.9 10.1 1.0 CD2 F:HIS10 3.1 19.5 1.0 CE1 F:HIS10 3.1 21.5 1.0 CD2 J:HIS10 3.3 16.7 1.0 ND1 J:HIS10 3.9 16.3 1.0 ND1 B:HIS10 3.9 13.1 1.0 CG B:HIS10 4.0 12.2 1.0 CG J:HIS10 4.2 16.9 1.0 ND1 F:HIS10 4.2 21.0 1.0 CG F:HIS10 4.2 21.4 1.0 O B:LEU6 4.7 18.2 1.0 O J:LEU6 4.7 23.4 1.0 O F:LEU6 4.8 17.5 1.0 CB B:LEU6 5.0 18.0 1.0

Zinc binding site 2 out of 2 in the Insulin, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Insulin, Monoclinic Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn31 b:19.8 occ:1.00 NE2 H:HIS10 1.8 14.2 1.0 NE2 L:HIS10 1.9 13.2 1.0 NE2 D:HIS10 2.2 17.9 1.0 CL H:CL31 2.3 21.1 1.0 CE1 L:HIS10 2.7 15.6 1.0 CE1 H:HIS10 2.8 16.4 1.0 CD2 H:HIS10 2.9 15.2 1.0 CD2 D:HIS10 3.1 17.1 1.0 CD2 L:HIS10 3.2 14.2 1.0 CE1 D:HIS10 3.2 18.0 1.0 ND1 L:HIS10 3.9 15.6 1.0 ND1 H:HIS10 3.9 16.9 1.0 CG H:HIS10 4.1 16.1 1.0 CG L:HIS10 4.1 16.3 1.0 CG D:HIS10 4.3 18.4 1.0 ND1 D:HIS10 4.3 17.5 1.0 O L:LEU6 4.7 24.1 1.0 O D:LEU6 4.7 20.3 1.0 O H:LEU6 4.7 19.5 1.0 O H:HOH33 4.9 25.9 1.0 CB L:LEU6 5.0 23.9 1.0 O L:HOH32 5.0 38.1 1.0

J.P.Turkenburg, J.L.Whittingham, U.Derewenda, Z.S.Derewenda, E.J.Dodson, G.G.Dodson, G.D.Smith, B.Xiao. Structure Determination and Refinement of Two Crystal Forms of Native Insulins To Be Published.