Zinc in PDB 1ro0: Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote

The structure of Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote, PDB code: 1ro0 was solved by G.Lipps, A.O.Weinzierl, G.Von Scheven, C.Buchen, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.80
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	47.532, 119.424, 41.943, 90.00, 90.00, 90.00
R / Rfree (%)	23.2 / 24.3

The binding sites of Zinc atom in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote (pdb code 1ro0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote, PDB code: 1ro0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:66.9 occ:1.00 ND1 A:HIS188 2.3 27.7 1.0 NE2 A:HIS141 2.5 35.6 1.0 SG A:CYS191 2.8 62.5 1.0 SG A:CYS196 2.8 45.6 1.0 CE1 A:HIS188 3.1 24.7 1.0 CD2 A:HIS141 3.3 32.8 1.0 CG A:HIS188 3.4 26.1 1.0 CE1 A:HIS141 3.5 36.1 1.0 CB A:CYS196 3.6 44.7 1.0 CB A:CYS191 3.7 53.1 1.0 CB A:HIS188 3.8 27.5 1.0 NE2 A:HIS188 4.3 28.7 1.0 CA A:HIS188 4.3 27.0 1.0 CG1 A:VAL186 4.4 30.6 1.0 CD2 A:HIS188 4.4 25.5 1.0 CG A:HIS141 4.5 32.0 1.0 CA A:CYS196 4.6 42.9 1.0 ND1 A:HIS141 4.6 35.4 1.0

Zinc binding site 2 out of 2 in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Semet Remote within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn302 b:49.8 occ:1.00 O A:HOH483 1.9 59.7 1.0 NE2 A:HIS53 2.2 36.9 1.0 OE2 A:GLU215 2.3 49.4 1.0 OE1 A:GLU215 2.5 51.1 1.0 CD2 A:HIS53 2.7 36.6 1.0 O A:HOH319 2.7 57.0 1.0 CD A:GLU215 2.7 46.7 1.0 CE1 A:HIS53 3.4 39.3 1.0 O A:HOH440 3.8 56.9 1.0 CG A:HIS53 4.0 36.6 1.0 CG A:GLU215 4.2 45.0 1.0 ND1 A:HIS53 4.3 38.3 1.0 NE1 A:TRP49 4.8 32.8 1.0 CB A:GLU215 4.9 38.1 1.0

G.Lipps, A.O.Weinzierl, G.Von Scheven, C.Buchen, P.Cramer. Structure of A Bifunctional Dna Primase-Polymerase Nat.Struct.Mol.Biol. V. 11 157 2004.
ISSN: ISSN 1545-9993
PubMed: 14730355
DOI: 10.1038/NSMB723