Atomistry » Zinc » PDB 1q66-1qmd » 1qf2
Atomistry »
  Zinc »
    PDB 1q66-1qmd »
      1qf2 »

Zinc in PDB 1qf2: Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase

Enzymatic activity of Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase

All present enzymatic activity of Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase:
3.4.24.27;

Protein crystallography data

The structure of Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase, PDB code: 1qf2 was solved by J.-F.Gaucher, M.Selkti, G.Tiraboschi, T.Prange, B.P.Roques, A.Tomas, M.C.Fournie-Zaluski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 10.00 / 2.06
Space group P 61 2 2
Cell size a, b, c (Å), α, β, γ (°) 93.500, 93.500, 131.300, 90.00, 90.00, 120.00
R / Rfree (%) 15.9 / 22.2

Other elements in 1qf2:

The structure of Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase also contains other interesting chemical elements:

Calcium (Ca) 4 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase (pdb code 1qf2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase, PDB code: 1qf2:

Zinc binding site 1 out of 1 in 1qf2

Go back to Zinc Binding Sites List in 1qf2
Zinc binding site 1 out of 1 in the Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Thermolysin (E.C.3.4.24.27) Complexed with (2-Sulphanyl-3- Phenylpropanoyl)-Gly-(5-Phenylproline). Parameters For Zn- Monodentation of Mercaptoacyldipeptides in Metalloendopeptidase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn320

b:7.4
occ:1.00
OE2 A:GLU166 1.9 11.4 1.0
NE2 A:HIS142 2.0 9.6 1.0
NE2 A:HIS146 2.1 8.9 1.0
SG A:TI3317 2.3 22.2 1.0
CD A:GLU166 2.8 8.8 1.0
OE1 A:GLU166 3.0 9.4 1.0
CD2 A:HIS146 3.0 7.1 1.0
CD2 A:HIS142 3.0 5.1 1.0
CE1 A:HIS142 3.1 5.9 1.0
CE1 A:HIS146 3.1 7.3 1.0
CA1 A:TI3317 3.3 21.6 1.0
OH A:TYR157 3.9 11.5 1.0
CG A:HIS146 4.2 9.0 1.0
ND1 A:HIS146 4.2 7.8 1.0
ND1 A:HIS142 4.2 5.0 1.0
CG A:HIS142 4.2 6.0 1.0
CG A:GLU166 4.2 5.9 1.0
NE2 A:HIS231 4.2 8.1 1.0
CB1 A:TI3317 4.2 20.0 1.0
C1 A:TI3317 4.4 21.0 1.0
C2 A:DMS326 4.5 51.7 1.0
OE1 A:GLU143 4.6 12.6 1.0
CB A:SER169 4.6 5.2 1.0
CD2 A:HIS231 4.7 7.7 1.0
O A:DMS326 4.8 55.0 1.0
OG A:SER169 4.8 6.6 1.0
O1 A:TI3317 4.8 19.1 1.0
CA A:GLU166 4.9 5.4 1.0
CZ A:TYR157 4.9 11.2 1.0

Reference:

J.F.Gaucher, M.Selkti, G.Tiraboschi, T.Prange, B.P.Roques, A.Tomas, M.C.Fournie-Zaluski. Crystal Structures of Alpha-Mercaptoacyldipeptides in the Thermolysin Active Site: Structural Parameters For A Zn Monodentation or Bidentation in Metalloendopeptidases. Biochemistry V. 38 12569 1999.
ISSN: ISSN 0006-2960
PubMed: 10504225
DOI: 10.1021/BI991043Z
Page generated: Wed Oct 16 18:08:24 2024

Last articles

Zn in 9MJ5
Zn in 9HNW
Zn in 9G0L
Zn in 9FNE
Zn in 9DZN
Zn in 9E0I
Zn in 9D32
Zn in 9DAK
Zn in 8ZXC
Zn in 8ZUF
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy