Zinc in PDB 1lat: Glucocorticoid Receptor Mutant/Dna Complex

The structure of Glucocorticoid Receptor Mutant/Dna Complex, PDB code: 1lat was solved by D.T.Gewirth, P.B.Sigler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	38.720, 76.070, 118.880, 90.00, 90.00, 90.00
R / Rfree (%)	19.5 / 28.1

The binding sites of Zinc atom in the Glucocorticoid Receptor Mutant/Dna Complex (pdb code 1lat). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Glucocorticoid Receptor Mutant/Dna Complex, PDB code: 1lat:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Glucocorticoid Receptor Mutant/Dna Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Glucocorticoid Receptor Mutant/Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1514 b:34.1 occ:1.00 SG A:CYS460 2.3 31.3 1.0 SG A:CYS443 2.3 32.0 1.0 SG A:CYS457 2.4 30.0 1.0 SG A:CYS440 2.4 34.1 1.0 CB A:CYS460 3.3 32.0 1.0 CB A:CYS440 3.4 31.3 1.0 CB A:CYS443 3.4 32.1 1.0 CB A:CYS457 3.7 32.0 1.0 N A:CYS443 3.9 35.5 1.0 N A:CYS457 4.0 37.4 1.0 NH1 A:ARG489 4.0 33.1 1.0 CA A:CYS443 4.2 34.2 1.0 N A:CYS460 4.3 29.3 1.0 CB A:ASP445 4.3 42.9 1.0 CA A:CYS460 4.4 28.0 1.0 CA A:CYS457 4.4 33.1 1.0 CB A:VAL442 4.6 29.5 1.0 C A:VAL442 4.7 36.3 1.0 C A:CYS443 4.7 35.7 1.0 N A:ASP445 4.8 40.5 1.0 CA A:CYS440 4.8 34.6 1.0 NH2 A:ARG496 4.8 25.0 1.0 CB A:THR456 4.9 42.5 1.0 C A:CYS457 4.9 33.8 1.0 N A:SER444 5.0 37.7 1.0 CZ A:ARG489 5.0 30.9 1.0 O A:CYS457 5.0 32.0 1.0

Zinc binding site 2 out of 4 in the Glucocorticoid Receptor Mutant/Dna Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Glucocorticoid Receptor Mutant/Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1515 b:50.8 occ:1.00 SG A:CYS482 2.2 47.4 1.0 SG A:CYS476 2.3 54.3 1.0 SG A:CYS495 2.3 51.4 1.0 SG A:CYS492 2.3 47.4 1.0 CB A:CYS482 3.2 49.6 1.0 CB A:CYS492 3.2 43.0 1.0 CB A:CYS476 3.3 60.0 1.0 CB A:CYS495 3.4 48.3 1.0 N A:CYS495 4.2 40.9 1.0 N A:CYS482 4.2 56.5 1.0 N A:GLY480 4.2 73.1 1.0 CA A:CYS495 4.3 45.3 1.0 CA A:CYS482 4.3 50.9 1.0 CA A:CYS476 4.4 64.3 1.0 N A:GLU479 4.5 81.8 1.0 O A:HOH199 4.5 47.9 1.0 CA A:GLY480 4.6 68.3 1.0 CA A:CYS492 4.6 44.0 1.0 C A:GLU479 4.7 76.9 1.0 CB A:TYR478 4.7 82.3 1.0 N A:LYS481 4.8 62.2 1.0 NH2 A:ARG498 4.8 67.6 1.0 CA A:GLU479 4.8 79.5 1.0 N A:LYS477 4.9 70.7 1.0 O A:CYS492 5.0 45.4 1.0

Zinc binding site 3 out of 4 in the Glucocorticoid Receptor Mutant/Dna Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Glucocorticoid Receptor Mutant/Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1514 b:33.4 occ:1.00 SG B:CYS457 2.3 31.3 1.0 SG B:CYS443 2.3 35.1 1.0 SG B:CYS460 2.3 29.4 1.0 SG B:CYS440 2.4 35.1 1.0 CB B:CYS440 3.2 36.0 1.0 CB B:CYS460 3.4 30.6 1.0 CB B:CYS457 3.4 33.7 1.0 CB B:CYS443 3.4 30.6 1.0 NH1 B:ARG489 3.7 40.5 1.0 N B:CYS443 3.8 35.4 1.0 N B:CYS457 3.9 35.3 1.0 CA B:CYS443 4.1 35.0 1.0 N B:CYS460 4.2 28.9 1.0 CA B:CYS457 4.3 28.6 1.0 CA B:CYS460 4.4 28.1 1.0 CB B:ASP445 4.4 43.8 1.0 CB B:VAL442 4.5 34.7 1.0 N B:SER444 4.7 41.5 1.0 N B:ASP445 4.7 42.0 1.0 C B:CYS443 4.7 37.7 1.0 C B:VAL442 4.7 34.9 1.0 CA B:CYS440 4.7 40.9 1.0 CZ B:ARG489 4.8 37.4 1.0 NH2 B:ARG496 4.8 17.3 1.0 C B:CYS457 4.9 27.4 1.0 CA B:VAL442 5.0 36.0 1.0 C B:THR456 5.0 37.4 1.0 O B:CYS457 5.0 26.2 1.0

Zinc binding site 4 out of 4 in the Glucocorticoid Receptor Mutant/Dna Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Glucocorticoid Receptor Mutant/Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1515 b:34.7 occ:1.00 SG B:CYS492 2.3 34.6 1.0 SG B:CYS482 2.3 38.8 1.0 SG B:CYS495 2.3 33.3 1.0 SG B:CYS476 2.4 43.3 1.0 CB B:CYS492 3.3 32.9 1.0 CB B:CYS482 3.4 35.7 1.0 CB B:CYS495 3.4 29.5 1.0 CB B:CYS476 3.4 35.7 1.0 CD1 B:TYR478 3.9 54.1 1.0 N B:CYS482 4.0 39.2 1.0 N B:CYS495 4.1 29.0 1.0 CA B:CYS476 4.2 38.5 1.0 O B:HOH183 4.3 40.2 1.0 CA B:CYS482 4.3 36.3 1.0 CA B:GLY480 4.3 45.2 1.0 CA B:CYS495 4.3 29.8 1.0 N B:LYS481 4.4 44.3 1.0 N B:GLY480 4.5 46.6 1.0 CE1 B:TYR478 4.5 55.3 1.0 O B:HOH274 4.5 26.5 1.0 C B:GLY480 4.7 44.5 1.0 CA B:CYS492 4.8 33.9 1.0 CG B:TYR478 4.8 52.6 1.0 CB B:TYR478 4.8 51.5 1.0 O B:HOH149 4.9 70.6 1.0 O B:HOH148 5.0 42.9 1.0 C B:CYS476 5.0 41.1 1.0 O B:CYS492 5.0 31.6 1.0 N B:TYR478 5.0 47.3 1.0

D.T.Gewirth, P.B.Sigler. The Basis For Half-Site Specificity Explored Through A Non-Cognate Steroid Receptor-Dna Complex. Nat.Struct.Biol. V. 2 386 1995.
ISSN: ISSN 1072-8368
PubMed: 7664096
DOI: 10.1038/NSB0595-386