Zinc in PDB 1jzs: Isoleucyl-Trna Synthetase Complexed with Mupirocin

All present enzymatic activity of Isoleucyl-Trna Synthetase Complexed with Mupirocin:
6.1.1.5;

The structure of Isoleucyl-Trna Synthetase Complexed with Mupirocin, PDB code: 1jzs was solved by T.Nakama, O.Nureki, S.Yokoyama, Riken Structuralgenomics/Proteomics Initiative (Rsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.50
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	160.640, 94.580, 125.900, 90.00, 126.30, 90.00
R / Rfree (%)	24.9 / 27.4

The binding sites of Zinc atom in the Isoleucyl-Trna Synthetase Complexed with Mupirocin (pdb code 1jzs). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Isoleucyl-Trna Synthetase Complexed with Mupirocin, PDB code: 1jzs:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Isoleucyl-Trna Synthetase Complexed with Mupirocin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Isoleucyl-Trna Synthetase Complexed with Mupirocin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1101 b:40.7 occ:1.00 SG A:CYS184 2.4 30.4 1.0 SG A:CYS392 2.4 28.4 1.0 SG A:CYS389 2.4 40.0 1.0 SG A:CYS181 2.5 44.1 1.0 CB A:CYS389 3.1 34.4 1.0 CB A:CYS184 3.5 29.0 1.0 CB A:CYS181 3.5 37.0 1.0 CB A:CYS392 3.9 28.9 1.0 O A:CYS392 3.9 45.0 1.0 CG A:ARG183 4.1 38.0 1.0 N A:CYS184 4.1 30.7 1.0 N A:CYS392 4.2 31.7 1.0 CA A:CYS184 4.4 27.8 1.0 CA A:CYS392 4.5 33.4 1.0 CA A:CYS389 4.6 34.0 1.0 C A:CYS392 4.7 38.0 1.0 NE A:ARG183 4.8 36.8 1.0 CB A:THR394 4.9 35.6 1.0 CA A:CYS181 4.9 36.0 1.0 CB A:ARG391 5.0 33.3 1.0 CD A:ARG183 5.0 37.0 1.0

Zinc binding site 2 out of 2 in the Isoleucyl-Trna Synthetase Complexed with Mupirocin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Isoleucyl-Trna Synthetase Complexed with Mupirocin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1102 b:0.9 occ:1.00 N A:GLY465 2.5 81.7 1.0 SG A:CYS502 3.0 75.2 1.0 SG A:CYS504 3.0 99.1 1.0 CB A:CYS504 3.0 93.5 1.0 C A:CYS464 3.1 82.2 1.0 O A:GLY465 3.1 82.7 1.0 CB A:CYS464 3.2 86.9 1.0 CA A:GLY465 3.3 81.7 1.0 CA A:CYS464 3.3 83.5 1.0 SG A:CYS461 3.4 71.5 1.0 N A:CYS464 3.4 81.9 1.0 C A:GLY465 3.6 80.7 1.0 O A:CYS464 3.9 81.0 1.0 CB A:CYS461 4.0 63.0 1.0 N A:CYS504 4.1 90.1 1.0 CA A:CYS504 4.2 92.3 1.0 SG A:CYS464 4.4 92.8 1.0 CB A:CYS502 4.5 74.4 1.0 C A:ALA463 4.5 81.0 1.0 CB A:ALA463 4.8 78.8 1.0 N A:LYS466 4.8 77.6 1.0

T.Nakama, O.Nureki, S.Yokoyama. Structural Basis For the Recognition of Isoleucyl-Adenylate and An Antibiotic, Mupirocin, By Isoleucyl-Trna Synthetase. J.Biol.Chem. V. 276 47387 2001.
ISSN: ISSN 0021-9258
PubMed: 11584022
DOI: 10.1074/JBC.M109089200