Zinc in PDB 1ju9: Horse Liver Alcohol Dehydrogenase VAL292SER Mutant

All present enzymatic activity of Horse Liver Alcohol Dehydrogenase VAL292SER Mutant:
1.1.1.1;

The structure of Horse Liver Alcohol Dehydrogenase VAL292SER Mutant, PDB code: 1ju9 was solved by J.K.Rubach, S.Ramaswamy, B.V.Plapp, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	46.271, 180.398, 46.176, 90.00, 106.65, 90.00
R / Rfree (%)	19.6 / 23.2

The binding sites of Zinc atom in the Horse Liver Alcohol Dehydrogenase VAL292SER Mutant (pdb code 1ju9). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Horse Liver Alcohol Dehydrogenase VAL292SER Mutant, PDB code: 1ju9:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Horse Liver Alcohol Dehydrogenase VAL292SER Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Horse Liver Alcohol Dehydrogenase VAL292SER Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn375 b:29.9 occ:1.00 NE2 A:HIS67 2.1 21.4 1.0 O A:HOH437 2.2 21.4 1.0 SG A:CYS174 2.3 23.1 1.0 SG A:CYS46 2.4 25.1 1.0 CD2 A:HIS67 3.0 21.0 1.0 CE1 A:HIS67 3.1 22.1 1.0 CB A:CYS46 3.3 23.3 1.0 CB A:CYS174 3.4 22.0 1.0 OG A:SER48 4.0 24.3 1.0 CB A:SER48 4.1 23.2 1.0 ND1 A:HIS67 4.2 21.2 1.0 CG A:HIS67 4.2 20.5 1.0 CA A:CYS46 4.8 23.5 1.0 CA A:CYS174 4.8 22.7 1.0 N A:SER48 4.9 23.7 1.0

Zinc binding site 2 out of 4 in the Horse Liver Alcohol Dehydrogenase VAL292SER Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Horse Liver Alcohol Dehydrogenase VAL292SER Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn376 b:25.7 occ:1.00 SG A:CYS103 2.2 22.3 1.0 SG A:CYS100 2.2 28.1 1.0 SG A:CYS111 2.4 24.6 1.0 SG A:CYS97 2.4 26.2 1.0 CB A:CYS111 3.2 22.3 1.0 CB A:CYS103 3.3 24.4 1.0 CB A:CYS97 3.4 25.7 1.0 CB A:CYS100 3.5 28.7 1.0 N A:CYS97 3.6 25.1 1.0 CA A:CYS111 3.7 22.9 1.0 N A:CYS100 3.8 29.8 1.0 N A:GLY98 3.9 27.5 1.0 CA A:CYS97 3.9 25.8 1.0 N A:LEU112 4.0 23.8 1.0 N A:CYS103 4.1 23.9 1.0 CA A:CYS100 4.2 28.7 1.0 C A:CYS111 4.2 23.5 1.0 CA A:CYS103 4.3 24.3 1.0 C A:CYS97 4.3 27.0 1.0 N A:LYS99 4.5 30.7 1.0 C A:GLN96 4.7 24.0 1.0 N A:LYS113 4.7 25.7 1.0 CG A:LYS113 4.8 28.4 1.0 O A:CYS100 4.9 26.6 1.0 C A:CYS100 4.9 27.9 1.0 CA A:GLY98 4.9 29.1 1.0 CA A:GLN96 5.0 23.2 1.0 C A:LYS99 5.0 31.4 1.0

Zinc binding site 3 out of 4 in the Horse Liver Alcohol Dehydrogenase VAL292SER Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Horse Liver Alcohol Dehydrogenase VAL292SER Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn375 b:30.3 occ:1.00 NE2 B:HIS67 2.0 20.8 1.0 O B:HOH433 2.1 20.5 1.0 SG B:CYS174 2.4 22.4 1.0 SG B:CYS46 2.4 25.6 1.0 CE1 B:HIS67 3.0 21.5 1.0 CD2 B:HIS67 3.0 20.7 1.0 CB B:CYS46 3.4 24.0 1.0 CB B:CYS174 3.4 22.2 1.0 CB B:SER48 4.1 24.6 1.0 OG B:SER48 4.1 26.0 1.0 ND1 B:HIS67 4.1 20.5 1.0 CG B:HIS67 4.2 21.4 1.0 CA B:CYS174 4.8 22.3 1.0 CA B:CYS46 4.9 23.8 1.0 N B:SER48 4.9 24.8 1.0 CE2 B:PHE93 4.9 18.6 1.0

Zinc binding site 4 out of 4 in the Horse Liver Alcohol Dehydrogenase VAL292SER Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Horse Liver Alcohol Dehydrogenase VAL292SER Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn376 b:25.6 occ:1.00 SG B:CYS103 2.2 22.8 1.0 SG B:CYS100 2.3 25.6 1.0 SG B:CYS97 2.4 26.8 1.0 SG B:CYS111 2.4 23.2 1.0 CB B:CYS111 3.2 21.8 1.0 CB B:CYS103 3.3 24.2 1.0 CB B:CYS100 3.4 27.1 1.0 CB B:CYS97 3.4 26.0 1.0 N B:CYS97 3.6 25.1 1.0 CA B:CYS111 3.7 22.9 1.0 N B:GLY98 3.8 27.8 1.0 N B:CYS100 3.9 29.0 1.0 CA B:CYS97 4.0 26.0 1.0 N B:LEU112 4.0 23.8 1.0 N B:CYS103 4.1 24.3 1.0 CA B:CYS100 4.2 26.9 1.0 C B:CYS111 4.2 23.3 1.0 CA B:CYS103 4.3 24.4 1.0 C B:CYS97 4.3 27.0 1.0 N B:LYS99 4.4 30.9 1.0 C B:GLN96 4.6 24.1 1.0 O B:CYS100 4.8 24.8 1.0 C B:CYS100 4.8 26.1 1.0 CA B:GLY98 4.8 29.9 1.0 CG B:LYS113 4.8 26.8 1.0 N B:LYS113 4.9 24.4 1.0 CA B:GLN96 4.9 24.1 1.0 N B:CYS111 5.0 21.5 1.0

J.K.Rubach, S.Ramaswamy, B.V.Plapp. Contributions of Valine-292 in the Nicotinamide Binding Site of Liver Alcohol Dehydrogenase and Dynamics to Catalysis. Biochemistry V. 40 12686 2001.
ISSN: ISSN 0006-2960
PubMed: 11601993
DOI: 10.1021/BI011540R