Zinc in PDB 8f1i: Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T)

All present enzymatic activity of Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T):
2.7.7.6;

The structure of Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T) (pdb code 8f1i). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T), PDB code: 8f1i:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T) within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:114.0 occ:1.00 SG J:CYS70 2.3 53.4 1.0 SG J:CYS88 2.3 64.5 1.0 SG J:CYS72 2.3 64.7 1.0 SG J:CYS85 2.3 77.4 1.0 CB J:CYS85 3.2 65.3 1.0 CB J:CYS72 3.4 55.9 1.0 N J:CYS72 3.7 59.3 1.0 CB J:CYS70 4.0 39.1 1.0 N J:CYS88 4.0 68.1 1.0 CB J:CYS88 4.0 65.0 1.0 CA J:CYS72 4.1 58.1 1.0 CB J:LYS87 4.2 72.3 1.0 N J:LEU71 4.4 48.4 1.0 N J:GLY73 4.5 58.9 1.0 CA J:CYS88 4.6 68.0 1.0 CA J:CYS85 4.6 63.8 1.0 C J:LYS87 4.6 72.0 1.0 N J:LYS87 4.7 72.7 1.0 CA J:LYS87 4.8 73.3 1.0 C J:CYS72 4.8 59.3 1.0 N J:LYS74 4.8 63.8 1.0 C J:LEU71 4.8 50.2 1.0 CB J:LYS74 4.9 68.6 1.0

Zinc binding site 2 out of 2 in the Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Sign Rna Polymerase Early-Melted Intermediate Bound to Mismatch Fragment DHSU36MM1 (-12T) within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1503 b:102.6 occ:1.00 SG J:CYS895 2.3 54.5 1.0 SG J:CYS898 2.3 71.6 1.0 SG J:CYS888 2.3 50.8 1.0 SG J:CYS814 2.3 75.9 1.0 CB J:CYS898 3.2 41.2 1.0 CB J:CYS814 3.3 68.6 1.0 CB J:CYS888 3.5 52.2 1.0 NH2 J:ARG883 3.7 58.4 1.0 CB J:CYS895 3.8 40.2 1.0 CA J:CYS888 3.9 56.5 1.0 N J:CYS814 4.2 66.8 1.0 N J:ASP889 4.3 66.8 1.0 CA J:CYS898 4.3 39.6 1.0 N J:CYS898 4.3 37.6 1.0 CA J:CYS814 4.4 70.6 1.0 N J:CYS895 4.4 27.7 1.0 OG1 J:THR890 4.5 59.0 1.0 C J:CYS888 4.6 58.2 1.0 CA J:CYS895 4.7 28.1 1.0 CZ J:ARG883 4.7 62.7 1.0 N J:THR890 5.0 63.8 1.0 NE J:ARG883 5.0 63.0 1.0

A.U.Mueller, J.Chen, M.Wu, C.Chiu, B.T.Nixon, E.A.Campbell, S.A.Darst. A General Mechanism For Transcription Bubble Nucleation in Bacteria. Proc.Natl.Acad.Sci.Usa V. 120 74120 2023.
ISSN: ESSN 1091-6490
PubMed: 36972428
DOI: 10.1073/PNAS.2220874120