Zinc in PDB 8eiy: Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide

Enzymatic activity of Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide

All present enzymatic activity of Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide:
1.1.1.1;

Protein crystallography data

The structure of Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide, PDB code: 8eiy was solved by C.Zheng, I.I.Mathews, S.G.Boxer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.09 / 2.55
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 55.1, 71.54, 92.44, 90, 102.95, 90
R / Rfree (%) 20.8 / 26.3

Other elements in 8eiy:

The structure of Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide also contains other interesting chemical elements:

Cobalt (Co) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide (pdb code 8eiy). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide, PDB code: 8eiy:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 8eiy

Go back to Zinc Binding Sites List in 8eiy
Zinc binding site 1 out of 2 in the Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn402

b:53.2
occ:1.00
 SG A:CYS100 2.3 64.2 1.0
SG A:CYS111 2.3 58.5 1.0
SG A:CYS97 2.3 59.7 1.0
SG A:CYS103 2.3 53.5 1.0
CB A:CYS111 3.2 56.8 1.0
CB A:CYS103 3.3 56.6 1.0
CB A:CYS100 3.4 57.3 1.0
N A:CYS97 3.4 55.0 1.0
CB A:CYS97 3.6 51.0 1.0
CA A:CYS111 3.8 55.5 1.0
N A:GLY98 3.9 56.8 1.0
N A:LEU112 4.0 61.1 1.0
CA A:CYS97 4.0 53.1 1.0
N A:CYS100 4.2 57.8 1.0
N A:CYS103 4.2 62.5 1.0
CA A:CYS103 4.4 60.2 1.0
C A:CYS111 4.4 58.9 1.0
C A:CYS97 4.4 52.4 1.0
CA A:CYS100 4.4 60.3 1.0
C A:GLN96 4.4 59.3 1.0
CA A:GLN96 4.6 53.0 1.0
CD1 A:LEU112 4.7 66.6 1.0
N A:LYS99 4.7 55.6 1.0
CA A:GLY98 4.9 57.0 1.0

Zinc binding site 2 out of 2 in 8eiy

Go back to Zinc Binding Sites List in 8eiy
Zinc binding site 2 out of 2 in the Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase in Complex with Nadh and N-Cyclohexylformamide within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn402

b:55.8
occ:1.00
 SG B:CYS103 2.3 57.0 1.0
SG B:CYS111 2.3 60.1 1.0
SG B:CYS100 2.3 52.7 1.0
SG B:CYS97 2.3 60.1 1.0
CB B:CYS103 3.3 66.5 1.0
CB B:CYS111 3.3 49.1 1.0
CB B:CYS97 3.4 60.4 1.0
N B:CYS97 3.5 55.5 1.0
CB B:CYS100 3.6 52.2 1.0
N B:GLY98 3.7 65.8 1.0
N B:CYS100 3.8 56.9 1.0
CA B:CYS97 3.9 60.8 1.0
CA B:CYS111 4.0 54.4 1.0
N B:CYS103 4.1 64.2 1.0
C B:CYS97 4.2 65.3 1.0
CA B:CYS103 4.2 68.1 1.0
CA B:CYS100 4.2 61.8 1.0
N B:LYS99 4.3 60.8 1.0
N B:LEU112 4.3 49.3 1.0
C B:GLN96 4.5 59.5 1.0
C B:CYS111 4.6 51.8 1.0
CA B:GLY98 4.6 61.0 1.0
CA B:GLN96 4.8 54.5 1.0
N B:LYS113 4.8 46.9 1.0
C B:GLY98 4.9 56.3 1.0
CG B:LYS113 4.9 60.4 1.0
C B:LYS99 4.9 62.7 1.0
C B:CYS100 4.9 60.6 1.0

Reference:

C.Zheng, I.I.Mathews, S.G.Boxer. Structure of Cobalt(II)-Substituted S48T Horse Liver Alcohol Dehydrogenase at 2.55 Angstroms Resolution To Be Published.
Page generated: Sat Apr 8 08:39:19 2023

Last articles

Zn in 2YRC
Zn in 2YQP
Zn in 2YR2
Zn in 2YQL
Zn in 2YPT
Zn in 2YPA
Zn in 2YPU
Zn in 2YNW
Zn in 2YNT
Zn in 2YNV
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy