Zinc in PDB 8egb: Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl

All present enzymatic activity of Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl:
2.7.7.6;

The structure of Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl (pdb code 8egb). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl, PDB code: 8egb:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:167.5 occ:1.00 SG J:CYS70 2.3 94.0 1.0 SG J:CYS85 2.3 97.2 1.0 SG J:CYS88 2.3 95.3 1.0 SG J:CYS72 2.3 98.3 1.0 CB J:CYS72 3.2 98.3 1.0 N J:CYS72 3.5 98.3 1.0 CB J:CYS85 3.5 97.2 1.0 CB J:CYS70 3.8 94.0 1.0 CB J:CYS88 3.8 95.3 1.0 N J:CYS88 3.9 95.3 1.0 CA J:CYS72 3.9 98.3 1.0 N J:LEU71 4.2 90.8 1.0 CA J:CYS88 4.2 95.3 1.0 N J:GLY89 4.3 91.5 1.0 C J:CYS88 4.4 95.3 1.0 C J:LEU71 4.5 90.8 1.0 N J:VAL90 4.6 84.1 1.0 CB J:LYS87 4.7 98.0 1.0 CG2 J:VAL90 4.7 84.1 1.0 CA J:LEU71 4.8 90.8 1.0 C J:CYS72 4.9 98.3 1.0 CA J:CYS85 4.9 97.2 1.0 CB J:LEU71 4.9 90.8 1.0 CB J:VAL90 4.9 84.1 1.0 C J:CYS70 4.9 94.0 1.0 C J:LYS87 4.9 98.0 1.0 CA J:CYS70 4.9 94.0 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Consensus Elemental Paused Elongation Complex with An Unfolded Tl within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1503 b:84.6 occ:1.00 SG J:CYS895 2.3 58.1 1.0 SG J:CYS898 2.3 57.6 1.0 SG J:CYS814 2.3 66.0 1.0 SG J:CYS888 2.3 61.8 1.0 CB J:CYS814 2.8 66.0 1.0 CB J:CYS888 3.1 61.8 1.0 CB J:CYS898 3.2 57.6 1.0 CB J:CYS895 3.6 58.1 1.0 CA J:CYS888 3.8 61.8 1.0 N J:CYS814 3.9 66.0 1.0 CA J:CYS814 4.0 66.0 1.0 N J:CYS895 4.2 58.1 1.0 OG1 J:THR890 4.3 59.9 1.0 CA J:CYS898 4.3 57.6 1.0 N J:CYS898 4.3 57.6 1.0 CA J:CYS895 4.4 58.1 1.0 C J:CYS888 4.7 61.8 1.0 N J:ASP889 4.7 68.9 1.0 O J:CYS895 4.8 58.1 1.0 OD1 J:ASP889 4.9 68.9 1.0 C J:CYS814 4.9 66.0 1.0 C J:CYS895 4.9 58.1 1.0

J.Y.Kang, T.V.Mishanina, Y.Bao, J.Chen, E.Llewellyn, J.Liu, S.A.Darst, R.Landick. An Ensemble of Interconverting Conformations of the Elemental Paused Transcription Complex Creates Regulatory Options. Proc.Natl.Acad.Sci.Usa V. 120 45120 2023.
ISSN: ESSN 1091-6490
PubMed: 36795753
DOI: 10.1073/PNAS.2215945120