Zinc in PDB 8c9d: Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue

All present enzymatic activity of Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue:
6.1.1.5;

The structure of Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue, PDB code: 8c9d was solved by A.Brkic, M.Leibundgut, J.Jablonska, V.Zanki, Z.Car, V.Petrovic Perokovic, N.Ban, I.Gruic-Sovulj, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.34 / 2.30
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	108.22, 108.22, 240.44, 90, 90, 90
R / Rfree (%)	20.1 / 24.1

The binding sites of Zinc atom in the Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue (pdb code 8c9d). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue, PDB code: 8c9d:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1101 b:49.2 occ:1.00 SG A:CYS181 2.3 45.0 1.0 SG A:CYS184 2.3 50.1 1.0 SG A:CYS387 2.3 48.2 1.0 SG A:CYS390 2.4 78.7 1.0 CB A:CYS387 3.1 49.3 1.0 CB A:CYS390 3.2 47.9 1.0 CB A:CYS184 3.3 42.8 1.0 CB A:CYS181 3.5 46.4 1.0 N A:CYS390 3.6 52.1 1.0 N A:CYS184 3.8 48.3 1.0 CA A:CYS390 4.0 50.7 1.0 O A:HOH1338 4.1 48.4 1.0 CA A:CYS184 4.2 39.8 1.0 CA A:CYS387 4.6 51.6 1.0 CB A:SER183 4.8 43.5 1.0 C A:CYS390 4.8 56.0 1.0 C A:ARG389 4.8 55.4 1.0 CA A:CYS181 4.8 46.2 1.0 N A:ASP391 4.9 54.7 1.0 C A:SER183 5.0 51.0 1.0

Zinc binding site 2 out of 2 in the Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Priestia Megaterium W130Q Mutant of Type 2 Isoleucyl-Trna Synthetase Complexed with An Isoleucyl-Adenylate Analogue within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1102 b:44.2 occ:1.00 SG A:CYS500 2.3 39.9 1.0 SG A:CYS462 2.3 44.2 1.0 SG A:CYS459 2.3 44.0 1.0 SG A:CYS503 2.3 43.4 1.0 CB A:CYS500 3.1 43.7 1.0 CB A:CYS459 3.2 38.8 1.0 CB A:CYS503 3.5 44.4 1.0 CB A:CYS462 3.5 41.1 1.0 N A:CYS503 3.7 45.6 1.0 N A:CYS462 3.8 46.6 1.0 CA A:CYS503 4.1 50.1 1.0 CA A:CYS462 4.2 50.5 1.0 CB A:VAL502 4.4 48.3 1.0 N A:GLY505 4.5 45.4 1.0 CA A:CYS500 4.6 37.8 1.0 CB A:HIS464 4.6 37.8 1.0 C A:CYS503 4.6 43.7 1.0 CA A:CYS459 4.6 40.3 1.0 C A:VAL502 4.7 49.6 1.0 C A:CYS462 4.7 40.3 1.0 N A:SER504 4.8 48.4 1.0 C A:GLY461 4.8 49.5 1.0 CA A:GLY505 4.9 50.0 1.0 N A:GLN463 4.9 46.5 1.0 CG1 A:VAL502 4.9 46.6 1.0 CA A:VAL502 4.9 46.0 1.0 N A:VAL502 4.9 50.2 1.0 N A:HIS464 5.0 40.5 1.0

A.Brkic, M.Leibundgut, J.Jablonska, V.Zanki, Z.Car, V.Petrovic Perokovic, N.Ban, I.Gruic-Sovulj. Antibiotic Hyper-Resistance in A Class I Aminoacyl-Trna Synthetase with Altered Active Site Signature Motif To Be Published.