Zinc in PDB 7xug: Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex

All present enzymatic activity of Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex:
2.7.7.6;

The structure of Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex (pdb code 7xug). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex, PDB code: 7xug:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1501 b:176.5 occ:1.00 SG J:CYS70 2.3 99.6 1.0 SG J:CYS85 2.3 103.6 1.0 SG J:CYS72 2.3 103.9 1.0 SG J:CYS88 2.3 100.2 1.0 CB J:CYS85 3.2 103.6 1.0 CB J:CYS72 3.7 103.9 1.0 CB J:CYS88 3.7 100.2 1.0 N J:CYS72 3.7 103.9 1.0 CB J:CYS70 4.0 99.6 1.0 N J:CYS88 4.0 100.2 1.0 CA J:CYS72 4.2 103.9 1.0 N J:LEU71 4.2 93.8 1.0 CA J:CYS88 4.4 100.2 1.0 CG2 J:VAL90 4.5 91.7 1.0 C J:LEU71 4.5 93.8 1.0 CA J:CYS85 4.6 103.6 1.0 N J:GLY89 4.7 95.5 1.0 CA J:LEU71 4.8 93.8 1.0 C J:CYS72 4.8 103.9 1.0 CB J:LEU71 4.9 93.8 1.0 CB J:LYS87 4.9 106.3 1.0 CB J:LYS74 4.9 105.4 1.0 CE J:LYS87 5.0 106.3 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:94.5 occ:1.00 SG J:CYS898 2.3 48.6 1.0 SG J:CYS895 2.3 45.8 1.0 SG J:CYS888 2.3 54.4 1.0 SG J:CYS814 2.3 63.7 1.0 CB J:CYS814 3.1 63.7 1.0 CB J:CYS898 3.1 48.6 1.0 CB J:CYS888 3.3 54.4 1.0 NH2 J:ARG883 3.6 59.5 1.0 CB J:CYS895 3.7 45.8 1.0 CA J:CYS888 3.7 54.4 1.0 N J:CYS814 3.9 63.7 1.0 N J:ASP889 4.1 61.0 1.0 CA J:CYS814 4.1 63.7 1.0 N J:CYS898 4.2 48.6 1.0 CA J:CYS898 4.2 48.6 1.0 N J:CYS895 4.3 45.8 1.0 C J:CYS888 4.4 54.4 1.0 OG1 J:THR816 4.5 71.9 1.0 OG1 J:THR890 4.5 56.1 1.0 CA J:CYS895 4.6 45.8 1.0 CZ J:ARG883 4.7 59.5 1.0 N J:THR890 4.8 56.1 1.0 N J:CYS888 4.9 54.4 1.0 O J:CYS895 5.0 45.8 1.0 C J:CYS814 5.0 63.7 1.0

S.Hwang, P.D.B.Olinares, J.Lee, J.Kim, B.T.Chait, R.A.King, J.Y.Kang. Structural Basis of Transcriptional Regulation By A Nascent Rna Element, HK022 Putrna. Nat Commun V. 13 4668 2022.
ISSN: ESSN 2041-1723
PubMed: 35970830
DOI: 10.1038/S41467-022-32315-Y