Zinc in PDB 7xue: Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex

All present enzymatic activity of Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex:
2.7.7.6;

The structure of Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex (pdb code 7xue). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex, PDB code: 7xue:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1501 b:88.0 occ:1.00 SG J:CYS70 2.3 64.3 1.0 SG J:CYS72 2.3 71.0 1.0 SG J:CYS85 2.3 71.6 1.0 SG J:CYS88 2.3 68.8 1.0 CB J:CYS85 3.1 71.6 1.0 CB J:CYS72 3.4 71.0 1.0 CB J:CYS88 3.6 68.8 1.0 N J:CYS88 3.8 68.8 1.0 N J:CYS72 3.8 71.0 1.0 CB J:CYS70 4.0 64.3 1.0 CB J:LYS87 4.1 83.5 1.0 CA J:CYS72 4.2 71.0 1.0 CA J:CYS88 4.2 68.8 1.0 N J:LEU71 4.5 62.6 1.0 CA J:CYS85 4.5 71.6 1.0 N J:GLY73 4.6 70.5 1.0 C J:LYS87 4.6 83.5 1.0 N J:LYS87 4.7 83.5 1.0 CA J:LYS87 4.7 83.5 1.0 N J:GLY89 4.8 64.2 1.0 CB J:LYS74 4.8 80.3 1.0 C J:CYS70 4.8 64.3 1.0 N J:LYS74 4.9 80.3 1.0 C J:CYS72 4.9 71.0 1.0 C J:CYS88 4.9 68.8 1.0 C J:LEU71 4.9 62.6 1.0 C J:CYS85 4.9 71.6 1.0 CG J:LYS87 4.9 83.5 1.0 CA J:CYS70 5.0 64.3 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:81.2 occ:1.00 SG J:CYS898 2.3 50.7 1.0 SG J:CYS814 2.3 59.7 1.0 SG J:CYS895 2.3 47.8 1.0 SG J:CYS888 2.3 54.3 1.0 CB J:CYS814 3.2 59.7 1.0 CB J:CYS898 3.2 50.7 1.0 CB J:CYS895 3.4 47.8 1.0 CB J:CYS888 3.4 54.3 1.0 NH2 J:ARG883 3.6 58.7 1.0 N J:CYS814 3.9 59.7 1.0 CA J:CYS888 4.0 54.3 1.0 CA J:CYS814 4.1 59.7 1.0 N J:CYS898 4.2 50.7 1.0 CA J:CYS898 4.3 50.7 1.0 N J:CYS895 4.3 47.8 1.0 CA J:CYS895 4.4 47.8 1.0 CZ J:ARG883 4.4 58.7 1.0 OG1 J:THR816 4.5 62.9 1.0 N J:ASP889 4.6 62.3 1.0 OG1 J:THR890 4.6 54.7 1.0 C J:CYS888 4.7 54.3 1.0 O J:CYS895 4.8 47.8 1.0 NE J:ARG883 4.8 58.7 1.0 C J:CYS895 4.9 47.8 1.0 C J:ASP813 5.0 56.5 1.0

S.Hwang, P.D.B.Olinares, J.Lee, J.Kim, B.T.Chait, R.A.King, J.Y.Kang. Structural Basis of Transcriptional Regulation By A Nascent Rna Element, HK022 Putrna. Nat Commun V. 13 4668 2022.
ISSN: ESSN 2041-1723
PubMed: 35970830
DOI: 10.1038/S41467-022-32315-Y