Zinc in PDB 7s7l: Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)

Enzymatic activity of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)

All present enzymatic activity of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD):
3.4.24.17;

Protein crystallography data

The structure of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD), PDB code: 7s7l was solved by M.Coban, M.Raeeszadeh-Sarmazdeh, A.Hockla, B.Sankaran, E.S.Radisky, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.60 / 2.34
Space group P 65 2 2
Cell size a, b, c (Å), α, β, γ (°) 69.332, 69.332, 312.215, 90, 90, 120
R / Rfree (%) 20.6 / 23.9

Other elements in 7s7l:

The structure of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) also contains other interesting chemical elements:

Chlorine (Cl) 1 atom
Calcium (Ca) 3 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) (pdb code 7s7l). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD), PDB code: 7s7l:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 7s7l

Go back to Zinc Binding Sites List in 7s7l
Zinc binding site 1 out of 2 in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn304

b:41.5
occ:1.00
 NE2 A:HIS211 2.0 35.8 1.0
NE2 A:HIS201 2.0 74.0 1.0
NE2 A:HIS205 2.1 45.6 1.0
N B:CYS1 2.2 68.9 1.0
O B:CYS1 2.3 46.4 1.0
CD2 A:HIS205 2.7 15.4 1.0
C B:CYS1 2.8 31.3 1.0
CD2 A:HIS211 2.8 34.3 1.0
CA B:CYS1 2.9 51.1 1.0
CD2 A:HIS201 3.0 30.3 1.0
CE1 A:HIS201 3.1 36.2 1.0
CE1 A:HIS211 3.1 36.0 1.0
CE1 A:HIS205 3.3 36.1 1.0
CB B:CYS1 3.4 42.5 1.0
CG A:HIS205 4.0 19.7 1.0
OE2 A:GLU202 4.0 52.0 1.0
N B:THR2 4.0 42.9 1.0
CG A:HIS211 4.1 34.3 1.0
CG A:HIS201 4.1 32.2 1.0
ND1 A:HIS211 4.1 30.7 1.0
ND1 A:HIS201 4.2 40.0 1.0
ND1 A:HIS205 4.2 32.4 1.0
OE1 A:GLU202 4.3 54.0 1.0
CD A:GLU202 4.5 53.3 1.0
O B:TYR67 4.7 39.8 1.0
CA A:PRO221 4.8 42.3 1.0
CE A:MET219 4.8 32.6 1.0
CA B:SER68 4.8 42.0 1.0
SG B:CYS1 4.8 63.2 1.0
CA B:THR2 4.8 48.8 1.0
O B:SER68 4.8 34.5 1.0
CB A:PRO221 4.9 35.2 1.0
OG1 B:THR2 4.9 39.2 1.0

Zinc binding site 2 out of 2 in 7s7l

Go back to Zinc Binding Sites List in 7s7l
Zinc binding site 2 out of 2 in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/M66S/E67Y/L133N/S155L) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn305

b:43.2
occ:1.00
 OD2 A:ASP153 2.0 45.0 1.0
ND1 A:HIS179 2.0 46.6 1.0
NE2 A:HIS151 2.0 43.4 1.0
NE2 A:HIS166 2.0 46.5 1.0
CE1 A:HIS166 2.9 31.8 1.0
CG A:HIS179 2.9 51.4 1.0
CG A:ASP153 3.0 49.2 1.0
CE1 A:HIS151 3.0 39.2 1.0
CE1 A:HIS179 3.0 40.7 1.0
CD2 A:HIS151 3.0 32.8 1.0
CD2 A:HIS166 3.1 47.2 1.0
CB A:HIS179 3.2 31.7 1.0
OD1 A:ASP153 3.6 66.9 1.0
CD2 A:HIS179 4.1 35.1 1.0
CB A:ASP153 4.1 50.6 1.0
NE2 A:HIS179 4.1 52.9 1.0
ND1 A:HIS166 4.1 38.6 1.0
OH A:TYR168 4.1 43.6 1.0
ND1 A:HIS151 4.1 47.4 1.0
CG A:HIS151 4.2 53.8 1.0
CG A:HIS166 4.2 47.7 1.0
O A:TYR155 4.2 38.2 1.0
CE1 A:TYR168 4.5 37.8 1.0
CZ A:PHE157 4.6 37.6 1.0
CE2 A:PHE157 4.7 42.2 1.0
CA A:HIS179 4.8 34.1 1.0
CZ A:TYR168 4.8 48.0 1.0

Reference:

M.Raeeszadeh-Sarmazdeh, M.Coban, S.Mahajan, A.Hockla, B.Sankaran, G.P.Downey, D.C.Radisky, E.S.Radisky. Engineering of Tissue Inhibitor of Metalloproteinases Timp-1 For Fine Discrimination Between Closely Related Stromelysins Mmp-3 and Mmp-10. J.Biol.Chem. V. 298 01654 2022.
ISSN: ESSN 1083-351X
PubMed: 35101440
DOI: 10.1016/J.JBC.2022.101654
Page generated: Wed Oct 30 10:38:58 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy