Zinc in PDB 7riw: Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide

Enzymatic activity of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide

All present enzymatic activity of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide, PDB code: 7riw was solved by J.Oh, P.B.Dervan, D.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.81 / 3.20
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 165.012, 222.435, 192.301, 90, 99.61, 90
R / Rfree (%) 22.8 / 26.5

Other elements in 7riw:

The structure of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide (pdb code 7riw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide, PDB code: 7riw:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in 7riw

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Zinc binding site 1 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1801

b:205.5
occ:1.00
SG A:CYS107 2.3 188.2 1.0
CB A:CYS110 2.3 178.8 1.0
SG A:CYS148 2.5 199.4 1.0
CB A:CYS148 2.6 198.3 1.0
CB A:CYS167 2.7 200.6 1.0
CB A:CYS107 2.9 182.6 1.0
N A:CYS110 3.4 184.2 1.0
CA A:CYS110 3.4 182.8 1.0
SG A:CYS167 3.6 205.5 1.0
SG A:CYS110 3.6 178.1 1.0
CA A:CYS167 3.9 196.1 1.0
N A:CYS167 4.0 184.1 1.0
CA A:CYS148 4.1 194.2 1.0
O A:CYS110 4.1 177.9 1.0
O A:CYS148 4.3 202.8 1.0
C A:CYS110 4.3 181.4 1.0
CA A:CYS107 4.4 178.6 1.0
N A:GLY168 4.6 202.0 1.0
C A:CYS167 4.6 198.2 1.0
C A:HIS109 4.6 183.5 1.0
C A:CYS148 4.6 197.9 1.0
NE2 A:GLN171 4.7 183.4 1.0
CB A:ASN169 4.7 185.3 1.0
N A:ASN169 4.8 188.6 1.0
N A:CYS148 4.8 187.9 1.0
C A:CYS107 4.9 175.3 1.0
CB A:HIS109 5.0 179.2 1.0
C A:GLY166 5.0 191.2 1.0

Zinc binding site 2 out of 8 in 7riw

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Zinc binding site 2 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1802

b:135.6
occ:1.00
NE2 A:HIS80 1.8 139.1 1.0
CB A:CYS70 2.4 146.3 1.0
SG A:CYS77 2.5 100.5 1.0
SG A:CYS67 2.5 156.3 1.0
CB A:CYS67 2.5 155.9 1.0
SG A:CYS70 2.6 144.7 1.0
CD2 A:HIS80 2.7 134.8 1.0
CE1 A:HIS80 2.8 134.5 1.0
CB A:CYS77 3.8 110.4 1.0
ND1 A:HIS80 3.8 132.8 1.0
CA A:CYS70 3.9 150.6 1.0
CG A:HIS80 3.9 131.3 1.0
CA A:CYS67 4.0 153.8 1.0
N A:GLN71 4.3 143.3 1.0
N A:GLN68 4.4 148.8 1.0
C A:CYS70 4.4 143.1 1.0
N A:CYS70 4.4 165.3 1.0
C A:CYS67 4.6 150.0 1.0
CA A:CYS77 4.8 112.2 1.0
O A:PRO78 4.9 120.7 1.0
N A:GLU72 4.9 133.0 1.0
N A:CYS67 4.9 156.3 1.0
O A:GLN68 5.0 162.7 1.0

Zinc binding site 3 out of 8 in 7riw

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Zinc binding site 3 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1301

b:149.3
occ:1.00
CB B:CYS1166 2.3 129.7 1.0
SG B:CYS1185 2.4 146.8 1.0
SG B:CYS1182 2.4 149.4 1.0
SG B:CYS1166 2.5 138.5 1.0
SG B:CYS1163 2.8 134.0 1.0
N B:CYS1166 3.1 127.5 1.0
CB B:CYS1163 3.2 133.1 1.0
CA B:CYS1166 3.2 126.3 1.0
CB B:CYS1185 3.3 149.7 1.0
CB B:CYS1182 3.4 149.5 1.0
C B:ILE1165 4.3 130.6 1.0
C B:CYS1166 4.4 126.9 1.0
N B:GLY1167 4.5 126.8 1.0
CA B:CYS1163 4.6 129.4 1.0
CB B:ILE1165 4.7 127.7 1.0
CA B:CYS1185 4.7 153.1 1.0
CA B:CYS1182 4.8 148.6 1.0
CG2 B:ILE1165 4.8 132.4 1.0
O B:LYS1183 4.8 145.5 1.0
CA B:ILE1165 4.8 127.7 1.0
N B:CYS1185 4.9 149.4 1.0
N B:ILE1165 4.9 125.3 1.0
C B:CYS1163 5.0 127.9 1.0
OG1 B:THR1170 5.0 128.4 1.0

Zinc binding site 4 out of 8 in 7riw

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Zinc binding site 4 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn401

b:91.8
occ:1.00
SG C:CYS92 2.2 87.6 1.0
SG C:CYS86 2.3 84.7 1.0
SG C:CYS88 2.4 69.4 1.0
SG C:CYS95 2.6 76.7 1.0
CB C:CYS95 3.0 69.8 1.0
CB C:CYS86 3.0 78.7 1.0
CB C:CYS92 3.1 84.8 1.0
N C:CYS92 3.3 83.3 1.0
CB C:CYS88 3.4 70.9 1.0
CA C:CYS92 3.7 77.6 1.0
N C:CYS95 4.0 70.6 1.0
N C:CYS88 4.1 71.8 1.0
CA C:CYS95 4.1 69.6 1.0
C C:HIS91 4.3 85.2 1.0
CA C:CYS88 4.4 71.7 1.0
CA C:CYS86 4.4 79.1 1.0
C C:CYS92 4.5 73.4 1.0
CA C:HIS91 4.6 79.1 1.0
C C:LYS94 4.6 87.6 1.0
C C:CYS86 4.7 84.0 1.0
N C:PHE87 4.7 79.7 1.0
O C:CYS92 4.8 68.9 1.0
N C:LYS94 5.0 73.4 1.0

Zinc binding site 5 out of 8 in 7riw

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Zinc binding site 5 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn201

b:112.2
occ:1.00
SG I:CYS10 2.3 101.3 1.0
SG I:CYS32 2.3 117.9 1.0
SG I:CYS29 2.5 104.6 1.0
CB I:CYS7 2.5 93.5 1.0
SG I:CYS7 2.5 104.2 1.0
CB I:CYS29 2.9 98.8 1.0
OG1 I:THR31 3.3 115.3 1.0
CB I:CYS32 3.8 115.8 1.0
N I:CYS32 3.9 110.2 1.0
CA I:CYS7 4.0 92.4 1.0
CB I:CYS10 4.0 106.5 1.0
CB I:TYR34 4.1 93.8 1.0
N I:CYS10 4.3 96.9 1.0
CA I:CYS32 4.4 112.5 1.0
CA I:CYS29 4.4 99.6 1.0
CB I:THR31 4.7 114.7 1.0
N I:CYS7 4.7 91.8 1.0
CA I:CYS10 4.7 100.7 1.0
C I:CYS7 4.8 100.9 1.0
C I:CYS32 4.9 113.3 1.0
CG I:TYR34 4.9 92.7 1.0

Zinc binding site 6 out of 8 in 7riw

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Zinc binding site 6 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn202

b:81.8
occ:1.00
SG I:CYS106 2.2 71.9 1.0
SG I:CYS78 2.2 68.0 1.0
SG I:CYS75 2.6 90.4 1.0
CB I:CYS75 2.8 84.3 1.0
SG I:CYS103 2.8 85.4 1.0
OG I:SER80 3.3 88.4 1.0
CB I:CYS103 3.3 81.1 1.0
CB I:CYS78 3.4 70.3 1.0
CB I:CYS106 3.4 83.3 1.0
N I:CYS78 3.4 74.3 1.0
CA I:CYS78 3.9 74.4 1.0
N I:CYS106 4.2 90.9 1.0
N I:HIS79 4.3 79.1 1.0
CA I:CYS75 4.3 81.6 1.0
C I:CYS78 4.3 83.6 1.0
CA I:CYS106 4.3 85.8 1.0
CB I:LYS77 4.4 76.2 1.0
N I:SER80 4.5 94.2 1.0
C I:LYS77 4.6 78.6 1.0
CB I:HIS108 4.6 73.1 1.0
CB I:SER80 4.6 85.6 1.0
CA I:CYS103 4.8 68.1 1.0
N I:LYS77 4.8 78.6 1.0
CA I:LYS77 4.8 73.4 1.0
C I:CYS75 4.9 84.2 1.0
C I:CYS106 4.9 80.6 1.0

Zinc binding site 7 out of 8 in 7riw

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Zinc binding site 7 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn101

b:63.5
occ:1.00
SG J:CYS10 2.3 61.4 1.0
SG J:CYS46 2.4 56.5 1.0
SG J:CYS7 2.5 60.9 1.0
SG J:CYS45 2.5 87.5 1.0
CB J:CYS7 2.9 56.1 1.0
N J:CYS46 3.0 79.4 1.0
CA J:CYS46 3.1 73.1 1.0
CB J:CYS46 3.2 66.3 1.0
C J:CYS45 3.4 71.3 1.0
CB J:CYS10 3.7 69.0 1.0
CB J:CYS45 3.8 72.5 1.0
O J:CYS45 3.9 67.7 1.0
N J:CYS10 3.9 59.4 1.0
CA J:CYS45 4.2 62.6 1.0
CA J:CYS10 4.4 66.6 1.0
NE J:ARG43 4.4 71.2 1.0
CA J:CYS7 4.4 52.5 1.0
C J:CYS46 4.6 69.0 1.0
N J:GLY11 4.7 63.0 1.0
CB J:ARG43 4.8 75.7 1.0
CB J:SER9 4.8 63.8 1.0
CB J:LYS12 4.8 52.2 1.0
N J:LYS12 4.9 50.7 1.0
CD J:ARG43 5.0 76.5 1.0
N J:CYS45 5.0 58.8 1.0
C J:CYS7 5.0 55.2 1.0

Zinc binding site 8 out of 8 in 7riw

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Zinc binding site 8 out of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Zn101

b:215.2
occ:1.00
SG L:CYS48 2.3 187.2 1.0
SG L:CYS51 2.4 186.8 1.0
SG L:CYS34 2.4 176.2 1.0
SG L:CYS31 2.4 169.8 1.0
CB L:CYS48 2.6 185.9 1.0
CB L:CYS31 2.9 155.6 1.0
CB L:CYS51 3.2 187.7 1.0
OG L:SER36 3.6 176.7 1.0
N L:CYS51 3.8 187.0 1.0
CB L:CYS34 3.9 185.8 1.0
CA L:CYS51 4.0 187.1 1.0
CA L:CYS48 4.1 185.4 1.0
N L:GLY52 4.2 161.1 1.0
CA L:CYS31 4.4 141.2 1.0
N L:CYS34 4.4 183.0 1.0
N L:HIS53 4.5 150.8 1.0
C L:CYS51 4.6 186.1 1.0
C L:CYS48 4.7 185.4 1.0
CB L:HIS53 4.7 153.6 1.0
CA L:CYS34 4.8 186.7 1.0
C L:ASP50 4.8 187.7 1.0
CB L:SER36 4.8 174.0 1.0
N L:CYS48 4.9 184.4 1.0
O L:CYS48 5.0 185.0 1.0

Reference:

J.Oh, T.Jia, J.Xu, J.Chong, P.B.Dervan, D.Wang. Rna Polymerase II Trapped on A Molecular Treadmill: Structural Basis of Persistent Transcriptional Arrest By A Minor Groove Dna Binder. Proc.Natl.Acad.Sci.Usa V. 119 2022.
ISSN: ESSN 1091-6490
PubMed: 35022237
DOI: 10.1073/PNAS.2114065119
Page generated: Wed Oct 30 10:22:51 2024

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