Zinc in PDB 7riw: Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Enzymatic activity of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
All present enzymatic activity of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide:
2.7.7.6;
Protein crystallography data
The structure of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide, PDB code: 7riw
was solved by
J.Oh,
P.B.Dervan,
D.Wang,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
47.81 /
3.20
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
165.012,
222.435,
192.301,
90,
99.61,
90
|
R / Rfree (%)
|
22.8 /
26.5
|
Other elements in 7riw:
The structure of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
(pdb code 7riw). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the
Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide, PDB code: 7riw:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Zinc binding site 1 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 1 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1801
b:205.5
occ:1.00
|
SG
|
A:CYS107
|
2.3
|
188.2
|
1.0
|
CB
|
A:CYS110
|
2.3
|
178.8
|
1.0
|
SG
|
A:CYS148
|
2.5
|
199.4
|
1.0
|
CB
|
A:CYS148
|
2.6
|
198.3
|
1.0
|
CB
|
A:CYS167
|
2.7
|
200.6
|
1.0
|
CB
|
A:CYS107
|
2.9
|
182.6
|
1.0
|
N
|
A:CYS110
|
3.4
|
184.2
|
1.0
|
CA
|
A:CYS110
|
3.4
|
182.8
|
1.0
|
SG
|
A:CYS167
|
3.6
|
205.5
|
1.0
|
SG
|
A:CYS110
|
3.6
|
178.1
|
1.0
|
CA
|
A:CYS167
|
3.9
|
196.1
|
1.0
|
N
|
A:CYS167
|
4.0
|
184.1
|
1.0
|
CA
|
A:CYS148
|
4.1
|
194.2
|
1.0
|
O
|
A:CYS110
|
4.1
|
177.9
|
1.0
|
O
|
A:CYS148
|
4.3
|
202.8
|
1.0
|
C
|
A:CYS110
|
4.3
|
181.4
|
1.0
|
CA
|
A:CYS107
|
4.4
|
178.6
|
1.0
|
N
|
A:GLY168
|
4.6
|
202.0
|
1.0
|
C
|
A:CYS167
|
4.6
|
198.2
|
1.0
|
C
|
A:HIS109
|
4.6
|
183.5
|
1.0
|
C
|
A:CYS148
|
4.6
|
197.9
|
1.0
|
NE2
|
A:GLN171
|
4.7
|
183.4
|
1.0
|
CB
|
A:ASN169
|
4.7
|
185.3
|
1.0
|
N
|
A:ASN169
|
4.8
|
188.6
|
1.0
|
N
|
A:CYS148
|
4.8
|
187.9
|
1.0
|
C
|
A:CYS107
|
4.9
|
175.3
|
1.0
|
CB
|
A:HIS109
|
5.0
|
179.2
|
1.0
|
C
|
A:GLY166
|
5.0
|
191.2
|
1.0
|
|
Zinc binding site 2 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 2 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1802
b:135.6
occ:1.00
|
NE2
|
A:HIS80
|
1.8
|
139.1
|
1.0
|
CB
|
A:CYS70
|
2.4
|
146.3
|
1.0
|
SG
|
A:CYS77
|
2.5
|
100.5
|
1.0
|
SG
|
A:CYS67
|
2.5
|
156.3
|
1.0
|
CB
|
A:CYS67
|
2.5
|
155.9
|
1.0
|
SG
|
A:CYS70
|
2.6
|
144.7
|
1.0
|
CD2
|
A:HIS80
|
2.7
|
134.8
|
1.0
|
CE1
|
A:HIS80
|
2.8
|
134.5
|
1.0
|
CB
|
A:CYS77
|
3.8
|
110.4
|
1.0
|
ND1
|
A:HIS80
|
3.8
|
132.8
|
1.0
|
CA
|
A:CYS70
|
3.9
|
150.6
|
1.0
|
CG
|
A:HIS80
|
3.9
|
131.3
|
1.0
|
CA
|
A:CYS67
|
4.0
|
153.8
|
1.0
|
N
|
A:GLN71
|
4.3
|
143.3
|
1.0
|
N
|
A:GLN68
|
4.4
|
148.8
|
1.0
|
C
|
A:CYS70
|
4.4
|
143.1
|
1.0
|
N
|
A:CYS70
|
4.4
|
165.3
|
1.0
|
C
|
A:CYS67
|
4.6
|
150.0
|
1.0
|
CA
|
A:CYS77
|
4.8
|
112.2
|
1.0
|
O
|
A:PRO78
|
4.9
|
120.7
|
1.0
|
N
|
A:GLU72
|
4.9
|
133.0
|
1.0
|
N
|
A:CYS67
|
4.9
|
156.3
|
1.0
|
O
|
A:GLN68
|
5.0
|
162.7
|
1.0
|
|
Zinc binding site 3 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 3 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn1301
b:149.3
occ:1.00
|
CB
|
B:CYS1166
|
2.3
|
129.7
|
1.0
|
SG
|
B:CYS1185
|
2.4
|
146.8
|
1.0
|
SG
|
B:CYS1182
|
2.4
|
149.4
|
1.0
|
SG
|
B:CYS1166
|
2.5
|
138.5
|
1.0
|
SG
|
B:CYS1163
|
2.8
|
134.0
|
1.0
|
N
|
B:CYS1166
|
3.1
|
127.5
|
1.0
|
CB
|
B:CYS1163
|
3.2
|
133.1
|
1.0
|
CA
|
B:CYS1166
|
3.2
|
126.3
|
1.0
|
CB
|
B:CYS1185
|
3.3
|
149.7
|
1.0
|
CB
|
B:CYS1182
|
3.4
|
149.5
|
1.0
|
C
|
B:ILE1165
|
4.3
|
130.6
|
1.0
|
C
|
B:CYS1166
|
4.4
|
126.9
|
1.0
|
N
|
B:GLY1167
|
4.5
|
126.8
|
1.0
|
CA
|
B:CYS1163
|
4.6
|
129.4
|
1.0
|
CB
|
B:ILE1165
|
4.7
|
127.7
|
1.0
|
CA
|
B:CYS1185
|
4.7
|
153.1
|
1.0
|
CA
|
B:CYS1182
|
4.8
|
148.6
|
1.0
|
CG2
|
B:ILE1165
|
4.8
|
132.4
|
1.0
|
O
|
B:LYS1183
|
4.8
|
145.5
|
1.0
|
CA
|
B:ILE1165
|
4.8
|
127.7
|
1.0
|
N
|
B:CYS1185
|
4.9
|
149.4
|
1.0
|
N
|
B:ILE1165
|
4.9
|
125.3
|
1.0
|
C
|
B:CYS1163
|
5.0
|
127.9
|
1.0
|
OG1
|
B:THR1170
|
5.0
|
128.4
|
1.0
|
|
Zinc binding site 4 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 4 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn401
b:91.8
occ:1.00
|
SG
|
C:CYS92
|
2.2
|
87.6
|
1.0
|
SG
|
C:CYS86
|
2.3
|
84.7
|
1.0
|
SG
|
C:CYS88
|
2.4
|
69.4
|
1.0
|
SG
|
C:CYS95
|
2.6
|
76.7
|
1.0
|
CB
|
C:CYS95
|
3.0
|
69.8
|
1.0
|
CB
|
C:CYS86
|
3.0
|
78.7
|
1.0
|
CB
|
C:CYS92
|
3.1
|
84.8
|
1.0
|
N
|
C:CYS92
|
3.3
|
83.3
|
1.0
|
CB
|
C:CYS88
|
3.4
|
70.9
|
1.0
|
CA
|
C:CYS92
|
3.7
|
77.6
|
1.0
|
N
|
C:CYS95
|
4.0
|
70.6
|
1.0
|
N
|
C:CYS88
|
4.1
|
71.8
|
1.0
|
CA
|
C:CYS95
|
4.1
|
69.6
|
1.0
|
C
|
C:HIS91
|
4.3
|
85.2
|
1.0
|
CA
|
C:CYS88
|
4.4
|
71.7
|
1.0
|
CA
|
C:CYS86
|
4.4
|
79.1
|
1.0
|
C
|
C:CYS92
|
4.5
|
73.4
|
1.0
|
CA
|
C:HIS91
|
4.6
|
79.1
|
1.0
|
C
|
C:LYS94
|
4.6
|
87.6
|
1.0
|
C
|
C:CYS86
|
4.7
|
84.0
|
1.0
|
N
|
C:PHE87
|
4.7
|
79.7
|
1.0
|
O
|
C:CYS92
|
4.8
|
68.9
|
1.0
|
N
|
C:LYS94
|
5.0
|
73.4
|
1.0
|
|
Zinc binding site 5 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 5 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn201
b:112.2
occ:1.00
|
SG
|
I:CYS10
|
2.3
|
101.3
|
1.0
|
SG
|
I:CYS32
|
2.3
|
117.9
|
1.0
|
SG
|
I:CYS29
|
2.5
|
104.6
|
1.0
|
CB
|
I:CYS7
|
2.5
|
93.5
|
1.0
|
SG
|
I:CYS7
|
2.5
|
104.2
|
1.0
|
CB
|
I:CYS29
|
2.9
|
98.8
|
1.0
|
OG1
|
I:THR31
|
3.3
|
115.3
|
1.0
|
CB
|
I:CYS32
|
3.8
|
115.8
|
1.0
|
N
|
I:CYS32
|
3.9
|
110.2
|
1.0
|
CA
|
I:CYS7
|
4.0
|
92.4
|
1.0
|
CB
|
I:CYS10
|
4.0
|
106.5
|
1.0
|
CB
|
I:TYR34
|
4.1
|
93.8
|
1.0
|
N
|
I:CYS10
|
4.3
|
96.9
|
1.0
|
CA
|
I:CYS32
|
4.4
|
112.5
|
1.0
|
CA
|
I:CYS29
|
4.4
|
99.6
|
1.0
|
CB
|
I:THR31
|
4.7
|
114.7
|
1.0
|
N
|
I:CYS7
|
4.7
|
91.8
|
1.0
|
CA
|
I:CYS10
|
4.7
|
100.7
|
1.0
|
C
|
I:CYS7
|
4.8
|
100.9
|
1.0
|
C
|
I:CYS32
|
4.9
|
113.3
|
1.0
|
CG
|
I:TYR34
|
4.9
|
92.7
|
1.0
|
|
Zinc binding site 6 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 6 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn202
b:81.8
occ:1.00
|
SG
|
I:CYS106
|
2.2
|
71.9
|
1.0
|
SG
|
I:CYS78
|
2.2
|
68.0
|
1.0
|
SG
|
I:CYS75
|
2.6
|
90.4
|
1.0
|
CB
|
I:CYS75
|
2.8
|
84.3
|
1.0
|
SG
|
I:CYS103
|
2.8
|
85.4
|
1.0
|
OG
|
I:SER80
|
3.3
|
88.4
|
1.0
|
CB
|
I:CYS103
|
3.3
|
81.1
|
1.0
|
CB
|
I:CYS78
|
3.4
|
70.3
|
1.0
|
CB
|
I:CYS106
|
3.4
|
83.3
|
1.0
|
N
|
I:CYS78
|
3.4
|
74.3
|
1.0
|
CA
|
I:CYS78
|
3.9
|
74.4
|
1.0
|
N
|
I:CYS106
|
4.2
|
90.9
|
1.0
|
N
|
I:HIS79
|
4.3
|
79.1
|
1.0
|
CA
|
I:CYS75
|
4.3
|
81.6
|
1.0
|
C
|
I:CYS78
|
4.3
|
83.6
|
1.0
|
CA
|
I:CYS106
|
4.3
|
85.8
|
1.0
|
CB
|
I:LYS77
|
4.4
|
76.2
|
1.0
|
N
|
I:SER80
|
4.5
|
94.2
|
1.0
|
C
|
I:LYS77
|
4.6
|
78.6
|
1.0
|
CB
|
I:HIS108
|
4.6
|
73.1
|
1.0
|
CB
|
I:SER80
|
4.6
|
85.6
|
1.0
|
CA
|
I:CYS103
|
4.8
|
68.1
|
1.0
|
N
|
I:LYS77
|
4.8
|
78.6
|
1.0
|
CA
|
I:LYS77
|
4.8
|
73.4
|
1.0
|
C
|
I:CYS75
|
4.9
|
84.2
|
1.0
|
C
|
I:CYS106
|
4.9
|
80.6
|
1.0
|
|
Zinc binding site 7 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 7 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 7 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn101
b:63.5
occ:1.00
|
SG
|
J:CYS10
|
2.3
|
61.4
|
1.0
|
SG
|
J:CYS46
|
2.4
|
56.5
|
1.0
|
SG
|
J:CYS7
|
2.5
|
60.9
|
1.0
|
SG
|
J:CYS45
|
2.5
|
87.5
|
1.0
|
CB
|
J:CYS7
|
2.9
|
56.1
|
1.0
|
N
|
J:CYS46
|
3.0
|
79.4
|
1.0
|
CA
|
J:CYS46
|
3.1
|
73.1
|
1.0
|
CB
|
J:CYS46
|
3.2
|
66.3
|
1.0
|
C
|
J:CYS45
|
3.4
|
71.3
|
1.0
|
CB
|
J:CYS10
|
3.7
|
69.0
|
1.0
|
CB
|
J:CYS45
|
3.8
|
72.5
|
1.0
|
O
|
J:CYS45
|
3.9
|
67.7
|
1.0
|
N
|
J:CYS10
|
3.9
|
59.4
|
1.0
|
CA
|
J:CYS45
|
4.2
|
62.6
|
1.0
|
CA
|
J:CYS10
|
4.4
|
66.6
|
1.0
|
NE
|
J:ARG43
|
4.4
|
71.2
|
1.0
|
CA
|
J:CYS7
|
4.4
|
52.5
|
1.0
|
C
|
J:CYS46
|
4.6
|
69.0
|
1.0
|
N
|
J:GLY11
|
4.7
|
63.0
|
1.0
|
CB
|
J:ARG43
|
4.8
|
75.7
|
1.0
|
CB
|
J:SER9
|
4.8
|
63.8
|
1.0
|
CB
|
J:LYS12
|
4.8
|
52.2
|
1.0
|
N
|
J:LYS12
|
4.9
|
50.7
|
1.0
|
CD
|
J:ARG43
|
5.0
|
76.5
|
1.0
|
N
|
J:CYS45
|
5.0
|
58.8
|
1.0
|
C
|
J:CYS7
|
5.0
|
55.2
|
1.0
|
|
Zinc binding site 8 out
of 8 in 7riw
Go back to
Zinc Binding Sites List in 7riw
Zinc binding site 8 out
of 8 in the Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 8 of Rna Polymerase II Elongation Complex Scaffold 2, Without Polyamide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
L:Zn101
b:215.2
occ:1.00
|
SG
|
L:CYS48
|
2.3
|
187.2
|
1.0
|
SG
|
L:CYS51
|
2.4
|
186.8
|
1.0
|
SG
|
L:CYS34
|
2.4
|
176.2
|
1.0
|
SG
|
L:CYS31
|
2.4
|
169.8
|
1.0
|
CB
|
L:CYS48
|
2.6
|
185.9
|
1.0
|
CB
|
L:CYS31
|
2.9
|
155.6
|
1.0
|
CB
|
L:CYS51
|
3.2
|
187.7
|
1.0
|
OG
|
L:SER36
|
3.6
|
176.7
|
1.0
|
N
|
L:CYS51
|
3.8
|
187.0
|
1.0
|
CB
|
L:CYS34
|
3.9
|
185.8
|
1.0
|
CA
|
L:CYS51
|
4.0
|
187.1
|
1.0
|
CA
|
L:CYS48
|
4.1
|
185.4
|
1.0
|
N
|
L:GLY52
|
4.2
|
161.1
|
1.0
|
CA
|
L:CYS31
|
4.4
|
141.2
|
1.0
|
N
|
L:CYS34
|
4.4
|
183.0
|
1.0
|
N
|
L:HIS53
|
4.5
|
150.8
|
1.0
|
C
|
L:CYS51
|
4.6
|
186.1
|
1.0
|
C
|
L:CYS48
|
4.7
|
185.4
|
1.0
|
CB
|
L:HIS53
|
4.7
|
153.6
|
1.0
|
CA
|
L:CYS34
|
4.8
|
186.7
|
1.0
|
C
|
L:ASP50
|
4.8
|
187.7
|
1.0
|
CB
|
L:SER36
|
4.8
|
174.0
|
1.0
|
N
|
L:CYS48
|
4.9
|
184.4
|
1.0
|
O
|
L:CYS48
|
5.0
|
185.0
|
1.0
|
|
Reference:
J.Oh,
T.Jia,
J.Xu,
J.Chong,
P.B.Dervan,
D.Wang.
Rna Polymerase II Trapped on A Molecular Treadmill: Structural Basis of Persistent Transcriptional Arrest By A Minor Groove Dna Binder. Proc.Natl.Acad.Sci.Usa V. 119 2022.
ISSN: ESSN 1091-6490
PubMed: 35022237
DOI: 10.1073/PNAS.2114065119
Page generated: Wed Oct 30 10:22:51 2024
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