Zinc in PDB 7py8: Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation)

All present enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation):
2.7.7.6;

The structure of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation) (pdb code 7py8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation), PDB code: 7py8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1502 b:167.7 occ:1.00 SG D:CYS70 2.3 138.1 1.0 SG D:CYS88 2.3 144.6 1.0 N D:CYS72 2.5 147.0 1.0 CB D:CYS88 2.5 144.6 1.0 CB D:CYS72 2.6 147.0 1.0 CA D:CYS72 3.0 147.0 1.0 SG D:CYS85 3.6 135.3 1.0 C D:LEU71 3.6 139.2 1.0 N D:CYS88 3.6 144.6 1.0 CA D:CYS88 3.7 144.6 1.0 N D:LEU71 3.7 139.2 1.0 CB D:CYS70 4.0 138.1 1.0 CA D:LEU71 4.0 139.2 1.0 CB D:LEU71 4.2 139.2 1.0 SG D:CYS72 4.2 147.0 1.0 CB D:CYS85 4.3 135.3 1.0 C D:CYS70 4.3 138.1 1.0 C D:CYS72 4.3 147.0 1.0 C D:LYS87 4.6 146.9 1.0 O D:LEU71 4.6 139.2 1.0 C D:CYS88 4.6 144.6 1.0 N D:GLY73 4.6 141.0 1.0 CA D:CYS70 4.6 138.1 1.0 CB D:LYS87 4.8 146.9 1.0 O D:CYS70 5.0 138.1 1.0 N D:GLY89 5.0 131.9 1.0

Zinc binding site 2 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in Less-Swiveled Conformation) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1503 b:158.1 occ:1.00 SG D:CYS898 2.3 105.5 1.0 SG D:CYS895 2.3 108.4 1.0 SG D:CYS814 2.3 124.6 1.0 SG D:CYS888 2.3 105.1 1.0 CB D:CYS814 2.8 124.6 1.0 NH2 D:ARG883 3.2 112.7 1.0 CB D:CYS898 3.5 105.5 1.0 CB D:CYS888 3.5 105.1 1.0 CB D:CYS895 3.6 108.4 1.0 N D:CYS814 3.9 124.6 1.0 CA D:CYS814 3.9 124.6 1.0 CA D:CYS888 4.0 105.1 1.0 N D:CYS895 4.3 108.4 1.0 N D:ASP889 4.4 118.5 1.0 CZ D:ARG883 4.5 112.7 1.0 CA D:CYS895 4.5 108.4 1.0 N D:CYS898 4.5 105.5 1.0 CA D:CYS898 4.6 105.5 1.0 OG1 D:THR890 4.6 114.9 1.0 OG1 D:THR816 4.6 135.4 1.0 O D:CYS895 4.7 108.4 1.0 C D:CYS888 4.8 105.1 1.0 C D:CYS814 4.9 124.6 1.0 C D:CYS895 4.9 108.4 1.0 NE D:ARG883 4.9 112.7 1.0 N D:GLY815 5.0 127.7 1.0

C.Zhu, X.Guo, P.Dumas, M.Takacs, M.Abdelkareem, A.Vanden Broeck, C.Saint-Andre, G.Papai, C.Crucifix, J.Ortiz, A.Weixlbaumer. Transcription Factors Modulate Rna Polymerase Conformational Equilibrium. Nat Commun V. 13 1546 2022.
ISSN: ESSN 2041-1723
PubMed: 35318334
DOI: 10.1038/S41467-022-29148-0