Zinc in PDB 7py6: Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation)

Enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation)

All present enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation):
2.7.7.6;

Other elements in 7py6:

The structure of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation) also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation) (pdb code 7py6). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation), PDB code: 7py6:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 7py6

Go back to Zinc Binding Sites List in 7py6
Zinc binding site 1 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1502

b:176.8
occ:1.00
SG D:CYS88 2.0 136.0 1.0
CB D:CYS88 2.1 136.0 1.0
N D:CYS88 2.3 136.0 1.0
SG D:CYS85 2.5 135.7 1.0
CB D:CYS72 2.5 131.5 1.0
CA D:CYS88 2.7 136.0 1.0
C D:LYS87 3.2 136.0 1.0
SG D:CYS72 3.4 131.5 1.0
CB D:LYS87 3.6 136.0 1.0
CB D:CYS85 3.7 135.7 1.0
CE D:LYS87 3.8 136.0 1.0
CA D:LYS87 3.8 136.0 1.0
CD D:LYS87 3.9 136.0 1.0
C D:CYS88 3.9 136.0 1.0
CA D:CYS72 3.9 131.5 1.0
N D:LYS87 4.0 136.0 1.0
O D:LYS87 4.1 136.0 1.0
NZ D:LYS87 4.1 136.0 1.0
N D:CYS72 4.1 131.5 1.0
N D:GLY89 4.2 124.6 1.0
CG D:LYS87 4.4 136.0 1.0
O D:CYS88 4.8 136.0 1.0
C D:CYS85 4.9 135.7 1.0
CA D:CYS85 4.9 135.7 1.0
C D:CYS72 4.9 131.5 1.0
O D:CYS85 4.9 135.7 1.0

Zinc binding site 2 out of 2 in 7py6

Go back to Zinc Binding Sites List in 7py6
Zinc binding site 2 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusa and Nusg (Nusa and Nusg Elongation Complex in Less-Swiveled Conformation) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1503

b:120.5
occ:1.00
SG D:CYS898 2.3 87.0 1.0
SG D:CYS888 2.3 89.8 1.0
SG D:CYS814 2.3 91.2 1.0
CB D:CYS895 2.5 85.6 1.0
CB D:CYS888 2.8 89.8 1.0
CB D:CYS814 2.9 91.2 1.0
CA D:CYS888 3.5 89.8 1.0
SG D:CYS895 3.5 85.6 1.0
CB D:CYS898 3.7 87.0 1.0
CA D:CYS895 3.8 85.6 1.0
N D:CYS895 3.9 85.6 1.0
N D:ASP889 4.1 97.0 1.0
C D:CYS888 4.3 89.8 1.0
CA D:CYS814 4.3 91.2 1.0
OG1 D:THR816 4.5 94.3 1.0
N D:CYS814 4.6 91.2 1.0
N D:CYS888 4.6 89.8 1.0
C D:CYS895 4.7 85.6 1.0
N D:CYS898 4.7 87.0 1.0
CA D:CYS898 4.8 87.0 1.0
CB D:THR816 4.9 94.3 1.0
N D:THR890 4.9 92.7 1.0
O D:CYS895 5.0 85.6 1.0

Reference:

C.Zhu, X.Guo, P.Dumas, M.Takacs, M.Abdelkareem, A.Vanden Broeck, C.Saint-Andre, G.Papai, C.Crucifix, J.Ortiz, A.Weixlbaumer. Transcription Factors Modulate Rna Polymerase Conformational Equilibrium. Nat Commun V. 13 1546 2022.
ISSN: ESSN 2041-1723
PubMed: 35318334
DOI: 10.1038/S41467-022-29148-0
Page generated: Wed Oct 30 09:26:30 2024

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