Zinc in PDB 7py1: Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec)

All present enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec):
2.7.7.6;

The structure of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec) (pdb code 7py1). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec), PDB code: 7py1:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1502 b:135.7 occ:1.00 N D:CYS72 2.2 121.3 1.0 SG D:CYS72 2.5 121.3 1.0 SG D:CYS70 2.5 117.2 1.0 CB D:CYS70 2.6 117.2 1.0 N D:LEU71 2.8 111.2 1.0 C D:CYS70 2.9 117.2 1.0 CA D:CYS72 2.9 121.3 1.0 CB D:CYS72 2.9 121.3 1.0 N D:GLY73 3.1 123.2 1.0 C D:LEU71 3.1 111.2 1.0 CA D:CYS70 3.3 117.2 1.0 C D:CYS72 3.4 121.3 1.0 O D:CYS70 3.5 117.2 1.0 CA D:LEU71 3.5 111.2 1.0 CB D:CYS88 4.0 114.0 1.0 O D:LEU71 4.2 111.2 1.0 SG D:CYS85 4.2 119.3 1.0 CB D:LEU71 4.3 111.2 1.0 CA D:GLY73 4.3 123.2 1.0 CG1 D:VAL90 4.3 94.5 1.0 N D:LYS74 4.4 123.0 1.0 N D:CYS70 4.4 117.2 1.0 CB D:CYS85 4.5 119.3 1.0 O D:CYS72 4.6 121.3 1.0 C D:GLY73 4.8 123.2 1.0 SG D:CYS88 4.9 114.0 1.0

Zinc binding site 2 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (the Consensus Nusg-Ec) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1503 b:99.1 occ:1.00 SG D:CYS888 2.4 69.3 1.0 SG D:CYS814 2.4 81.4 1.0 SG D:CYS898 2.4 57.1 1.0 SG D:CYS895 2.5 59.9 1.0 CB D:CYS814 2.5 81.4 1.0 CB D:CYS888 3.2 69.3 1.0 CA D:CYS888 3.3 69.3 1.0 CA D:CYS814 3.7 81.4 1.0 N D:ASP889 3.7 77.0 1.0 N D:CYS814 3.7 81.4 1.0 CB D:CYS895 3.8 59.9 1.0 CB D:CYS898 3.8 57.1 1.0 C D:CYS888 4.0 69.3 1.0 N D:CYS895 4.2 59.9 1.0 N D:CYS888 4.4 69.3 1.0 CA D:CYS895 4.5 59.9 1.0 C D:CYS814 4.8 81.4 1.0 NH1 D:ARG883 4.8 74.0 1.0 N D:CYS898 4.9 57.1 1.0 CA D:CYS898 4.9 57.1 1.0 C D:ASP813 4.9 73.5 1.0 CA D:ASP889 5.0 77.0 1.0 O D:SER887 5.0 67.1 1.0

C.Zhu, X.Guo, P.Dumas, M.Takacs, M.Abdelkareem, A.Vanden Broeck, C.Saint-Andre, G.Papai, C.Crucifix, J.Ortiz, A.Weixlbaumer. Transcription Factors Modulate Rna Polymerase Conformational Equilibrium. Nat Commun V. 13 1546 2022.
ISSN: ESSN 2041-1723
PubMed: 35318334
DOI: 10.1038/S41467-022-29148-0