Zinc in PDB 7jhp: Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase

All present enzymatic activity of Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase:
2.7.11.1;

The structure of Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase, PDB code: 7jhp was solved by T.Cookis, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.61 / 2.77
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	94.487, 44.353, 74.395, 90.00, 103.95, 90.00
R / Rfree (%)	21.1 / 26.4

The structure of Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase (pdb code 7jhp). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase, PDB code: 7jhp:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn201 b:40.1 occ:1.00 ND1 C:HIS173 2.1 48.5 1.0 SG C:CYS155 2.5 42.3 1.0 SG C:CYS152 2.5 42.2 1.0 SG C:CYS176 2.6 40.3 1.0 CE1 C:HIS173 3.0 43.6 1.0 CB C:CYS152 3.0 46.3 1.0 CB C:CYS176 3.2 30.3 1.0 CG C:HIS173 3.2 44.5 1.0 CB C:HIS173 3.6 41.0 1.0 CB C:CYS155 3.7 41.6 1.0 N C:HIS173 3.8 41.9 1.0 N C:CYS155 3.9 40.1 1.0 NE2 C:HIS173 4.2 39.0 1.0 CD2 C:HIS173 4.3 44.5 1.0 CA C:HIS173 4.3 46.0 1.0 CA C:CYS155 4.4 40.6 1.0 CB C:ILE154 4.5 36.1 1.0 CE1 C:HIS175 4.5 62.0 1.0 CA C:CYS152 4.5 44.7 1.0 CA C:CYS176 4.7 44.1 1.0 C C:PHE172 4.8 41.9 1.0 CA C:PHE172 4.9 43.2 1.0 N C:ILE154 4.9 42.2 1.0 C C:ILE154 4.9 47.8 1.0 NE2 C:HIS175 5.0 62.1 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Hras in Complex with the Ras-Binding and Cysteine-Rich Domains of Craf-Kinase within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn202 b:63.0 occ:1.00 ND1 C:HIS139 2.3 49.8 1.0 CB C:CYS168 2.4 98.8 1.0 SG C:CYS165 2.4 64.8 1.0 SG C:CYS184 2.5 71.6 1.0 CB C:CYS165 3.0 65.5 1.0 CE1 C:HIS139 3.2 50.0 1.0 N C:CYS168 3.2 89.5 1.0 CG C:HIS139 3.4 48.6 1.0 CA C:CYS168 3.4 92.3 1.0 CB C:CYS184 3.5 69.4 1.0 SG C:CYS168 3.7 0.2 1.0 CB C:HIS139 3.7 50.8 1.0 CA C:HIS139 3.9 57.5 1.0 C C:THR167 4.3 82.1 1.0 O C:CYS165 4.3 83.6 1.0 NE2 C:HIS139 4.3 44.1 1.0 CA C:CYS165 4.4 71.6 1.0 CD2 C:HIS139 4.5 47.9 1.0 CA C:CYS184 4.5 68.8 1.0 C C:CYS168 4.5 92.0 1.0 CB C:THR167 4.5 70.7 1.0 C C:CYS165 4.6 78.1 1.0 N C:ASN140 4.6 62.6 1.0 CG2 C:THR167 4.7 71.5 1.0 CA C:THR167 4.8 81.6 1.0 N C:GLY169 4.8 80.1 1.0 N C:THR167 4.8 84.9 1.0 C C:HIS139 4.8 60.1 1.0 N C:HIS139 4.9 64.0 1.0

T.Cookis, C.Mattos. Crystal Structure Reveals the Full Ras:Raf Interface and Advances Mechanistic Understanding of Raf Activation Biorxiv 2020.
DOI: 10.1101/2020.07.28.225938