Zinc in PDB 7c2j: Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization)

All present enzymatic activity of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization):
2.7.7.48; 3.4.19.12; 3.4.22.69; 3.6.4.12; 3.6.4.13;

The structure of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization), PDB code: 7c2j was solved by S.Lin, H.Chen, F.Ye, Z.M.Chen, F.L.Yang, Y.Zheng, Y.Cao, J.X.Qiao, S.Y.Yang, G.W.Lu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.88 / 2.80
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	167.534, 167.534, 51.690, 90.00, 90.00, 120.00
R / Rfree (%)	20.2 / 23.5

The binding sites of Zinc atom in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization) (pdb code 7c2j). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization), PDB code: 7c2j:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:42.9 occ:1.00 SG B:CYS128 2.2 43.6 1.0 SG B:CYS117 2.2 41.9 1.0 SG B:CYS120 2.4 40.4 1.0 SG B:CYS130 2.6 46.7 1.0 CB B:CYS117 2.9 39.7 1.0 CB B:CYS130 3.4 59.7 1.0 CB B:CYS128 3.5 41.0 1.0 CB B:CYS120 3.8 40.3 1.0 N B:CYS120 3.9 41.1 1.0 O B:CYS120 4.0 71.2 1.0 N B:CYS130 4.1 72.6 1.0 CA B:CYS128 4.2 46.8 1.0 CA B:CYS120 4.3 39.5 1.0 CA B:CYS130 4.4 66.5 1.0 CA B:CYS117 4.4 38.7 1.0 C B:CYS120 4.5 46.5 1.0 N B:SER129 4.6 51.2 1.0 CB B:VAL119 4.7 48.8 1.0 C B:CYS128 4.7 43.4 1.0 CE B:LYS124 4.8 71.5 1.0 NZ B:LYS124 5.0 74.8 1.0 C B:VAL119 5.0 41.6 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (Without Additional Sam During Crystallization) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:23.1 occ:1.00 NE2 B:HIS83 2.0 25.1 1.0 SG B:CYS90 2.2 23.1 1.0 SG B:CYS74 2.2 24.0 1.0 SG B:CYS77 2.2 21.6 1.0 CD2 B:HIS83 2.9 32.4 1.0 CE1 B:HIS83 3.0 25.2 1.0 CB B:CYS74 3.1 22.7 1.0 CB B:CYS90 3.3 25.6 1.0 CB B:CYS77 3.4 21.5 1.0 N B:CYS77 3.7 24.0 1.0 CG B:HIS83 4.0 27.4 1.0 CA B:CYS77 4.1 22.7 1.0 ND1 B:HIS83 4.1 26.6 1.0 CA B:CYS90 4.4 25.4 1.0 CA B:CYS74 4.5 23.1 1.0 CB B:TYR76 4.7 27.4 1.0 C B:TYR76 4.7 25.4 1.0 CB B:LYS93 4.8 19.6 1.0 N B:LYS93 4.8 20.2 1.0 C B:CYS74 4.9 23.8 1.0

S.Lin, H.Chen, F.Ye, Z.M.Chen, F.L.Yang, Y.Zheng, Y.Cao, J.X.Qiao, S.Y.Yang, G.W.Lu. Crystal Structure of Sars-Cov-2 NSP10/NSP16 2'-O-Methylase and Its Implication on Antiviral Drug Design To Be Published.