Zinc in PDB 7c2i: Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization)

All present enzymatic activity of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization):
2.7.7.48; 3.4.19.12; 3.4.22.69; 3.6.4.12; 3.6.4.13;

The structure of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization), PDB code: 7c2i was solved by S.Lin, H.Chen, F.Ye, Z.M.Chen, F.L.Yang, Y.Zheng, Y.Cao, J.X.Qiao, S.Y.Yang, G.W.Lu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.94 / 2.50
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	167.817, 167.817, 51.571, 90.00, 90.00, 120.00
R / Rfree (%)	17.4 / 20.9

The binding sites of Zinc atom in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization) (pdb code 7c2i). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization), PDB code: 7c2i:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:63.5 occ:1.00 SG B:CYS128 2.2 53.9 1.0 SG B:CYS117 2.3 55.1 1.0 SG B:CYS130 2.3 60.7 1.0 SG B:CYS120 2.4 51.3 1.0 CB B:CYS117 3.1 52.2 1.0 CB B:CYS130 3.3 67.9 1.0 CB B:CYS128 3.5 55.8 1.0 CB B:CYS120 3.7 49.1 1.0 N B:CYS120 3.8 54.3 1.0 O B:CYS120 4.0 62.7 1.0 N B:CYS130 4.1 71.8 1.0 CA B:CYS128 4.1 59.1 1.0 CA B:CYS120 4.2 57.3 1.0 CA B:CYS130 4.3 73.0 1.0 NZ B:LYS124 4.3 69.0 1.0 CE B:LYS124 4.4 68.7 1.0 N B:SER129 4.5 58.2 1.0 C B:CYS120 4.5 58.3 1.0 CA B:CYS117 4.5 49.0 1.0 CB B:VAL119 4.6 58.6 1.0 C B:CYS128 4.7 57.1 1.0 C B:VAL119 4.9 55.6 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of NSP16-NSP10 Heterodimer From Sars-Cov-2 in Complex with Sam (with Additional Sam During Crystallization) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:30.9 occ:1.00 NE2 B:HIS83 2.1 44.1 1.0 SG B:CYS90 2.1 34.9 1.0 SG B:CYS74 2.2 30.6 1.0 SG B:CYS77 2.3 29.1 1.0 CE1 B:HIS83 3.0 43.4 1.0 CD2 B:HIS83 3.1 46.3 1.0 CB B:CYS74 3.1 28.5 1.0 CB B:CYS90 3.3 41.6 1.0 CB B:CYS77 3.4 27.9 1.0 N B:CYS77 3.6 33.8 1.0 CA B:CYS77 4.0 31.6 1.0 ND1 B:HIS83 4.2 49.5 1.0 CG B:HIS83 4.2 48.6 1.0 CA B:CYS90 4.4 48.0 1.0 CB B:TYR76 4.6 38.4 1.0 CA B:CYS74 4.6 28.1 1.0 C B:TYR76 4.6 33.6 1.0 CB B:LYS93 4.8 27.5 1.0 C B:CYS74 4.9 29.4 1.0 CA B:TYR76 5.0 33.9 1.0 N B:LYS93 5.0 28.9 1.0 N B:TYR76 5.0 30.9 1.0 O B:CYS74 5.0 32.2 1.0

S.Lin, H.Chen, F.Ye, Z.M.Chen, F.L.Yang, Y.Zheng, Y.Cao, J.X.Qiao, S.Y.Yang, G.W.Lu. Crystal Structure of Sars-Cov-2 NSP10/NSP16 2'-O-Methylase and Its Implication on Antiviral Drug Design To Be Published.