Zinc in PDB 7auw: Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B.

All present enzymatic activity of Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B.:
3.4.24.63;

The structure of Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B., PDB code: 7auw was solved by U.Eckhard, F.X.Gomis-Ruth, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	76.26 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	126.66, 88.24, 156.66, 90, 113.82, 90
R / Rfree (%)	23.6 / 26.1

The structure of Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B. also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Zinc atom in the Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B. (pdb code 7auw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B., PDB code: 7auw:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn702 b:97.3 occ:1.00 OD1 B:ASP156 1.9 107.1 1.0 NE2 A:HIS152 2.1 89.3 1.0 NE2 A:HIS162 2.1 93.7 1.0 NE2 A:HIS156 2.1 92.2 1.0 OD2 B:ASP156 2.1 109.9 1.0 CG B:ASP156 2.3 107.5 1.0 CE1 A:HIS162 2.8 93.7 1.0 CD2 A:HIS152 3.0 88.3 1.0 CE1 A:HIS156 3.1 92.7 1.0 CD2 A:HIS156 3.1 91.4 1.0 CE1 A:HIS152 3.1 89.6 1.0 CD2 A:HIS162 3.3 92.1 1.0 CB B:ASP156 3.8 104.4 1.0 ND1 A:HIS162 3.9 92.8 1.0 CG A:HIS162 4.2 90.6 1.0 CG A:HIS152 4.2 87.5 1.0 ND1 A:HIS156 4.2 92.5 1.0 ND1 A:HIS152 4.2 89.0 1.0 CG A:HIS156 4.2 90.7 1.0 OE2 A:GLU153 4.3 100.1 1.0 OE1 A:GLU153 4.6 99.7 1.0 N B:ASP156 4.6 102.7 1.0 CA B:ASP156 4.7 103.0 1.0 CD A:GLU153 4.7 98.7 1.0 CB B:PRO155 4.8 103.1 1.0 CE A:MET209 4.8 91.1 1.0 CE1 A:TYR211 4.9 96.4 1.0

Zinc binding site 2 out of 2 in the Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Inhibitory Complex of Human Meprin Beta with Mouse Fetuin-B. within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn702 b:90.9 occ:1.00 OD1 D:ASP156 1.9 106.8 1.0 NE2 C:HIS152 2.1 68.3 1.0 OD2 D:ASP156 2.1 107.5 1.0 NE2 C:HIS162 2.1 90.8 1.0 NE2 C:HIS156 2.1 91.9 1.0 CG D:ASP156 2.3 106.2 1.0 CE1 C:HIS162 2.7 90.8 1.0 CD2 C:HIS152 3.0 67.4 1.0 CE1 C:HIS156 3.1 91.8 1.0 CD2 C:HIS156 3.1 90.9 1.0 CE1 C:HIS152 3.1 68.4 1.0 CD2 C:HIS162 3.3 89.2 1.0 CB D:ASP156 3.8 102.9 1.0 ND1 C:HIS162 3.9 90.2 1.0 OE2 C:GLU153 4.1 93.1 1.0 CG C:HIS162 4.2 87.7 1.0 CG C:HIS152 4.2 67.5 1.0 ND1 C:HIS156 4.2 91.3 1.0 CG C:HIS156 4.2 89.5 1.0 ND1 C:HIS152 4.2 68.3 1.0 CD C:GLU153 4.6 89.4 1.0 N D:ASP156 4.6 101.0 1.0 OE1 C:GLU153 4.7 88.0 1.0 CA D:ASP156 4.7 101.5 1.0 CE C:MET209 4.8 73.9 1.0 CB D:PRO155 4.8 102.6 1.0 CE1 C:TYR211 4.9 81.1 1.0

U.Eckhard, H.Korschgen, N.Von Wiegen, W.Stocker, F.X.Gomis-Ruth. The Crystal Structure of A 250-kDa Heterotetrameric Particle Explains Inhibition of Sheddase Meprin Beta By Endogenous Fetuin-B. Proc.Natl.Acad.Sci.Usa V. 118 2021.
ISSN: ESSN 1091-6490
PubMed: 33782129
DOI: 10.1073/PNAS.2023839118