Zinc in PDB 5byh: Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex

All present enzymatic activity of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex:
2.7.7.6;

The structure of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex, PDB code: 5byh was solved by X.Zhang, M.Buck, V.C.Darbari, Y.Yang, N.Zhang, D.Lu, R.Glyde, Y.Wang, J.Winkelman, R.L.Gourse, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.64 / 3.76
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	208.480, 151.520, 195.280, 90.00, 90.00, 90.00
R / Rfree (%)	34.7 / 35.3

The binding sites of Zinc atom in the Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex (pdb code 5byh). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex, PDB code: 5byh:

Zinc binding site 1 out of 1 in the Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Escherichia Coli Rna Polymerase - SIGMA54 Holoenzyme Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1501 b:0.4 occ:1.00 SG D:CYS72 2.1 0.3 1.0 SG D:CYS70 2.2 0.7 1.0 SG D:CYS85 2.4 1.0 1.0 CB D:CYS85 3.3 0.1 1.0 SG D:CYS88 3.4 0.1 1.0 CB D:CYS72 3.5 0.8 1.0 CB D:CYS70 3.7 0.4 1.0 N D:CYS72 3.8 0.5 1.0 CA D:CYS72 4.2 0.8 1.0 N D:CYS88 4.3 0.4 1.0 CB D:CYS88 4.4 0.4 1.0 CB D:LYS87 4.5 0.2 1.0 N D:GLY73 4.5 0.1 1.0 N D:LEU71 4.5 0.4 1.0 CA D:CYS85 4.8 0.9 1.0 CA D:CYS70 4.8 1.0 1.0 C D:CYS72 4.9 0.1 1.0 C D:LEU71 5.0 0.1 1.0 CA D:CYS88 5.0 0.3 1.0 N D:LYS87 5.0 0.8 1.0

Y.Yang, V.C.Darbari, N.Zhang, D.Lu, R.Glyde, Y.P.Wang, J.T.Winkelman, R.L.Gourse, K.S.Murakami, M.Buck, X.Zhang. Transcription. Structures of the Rna Polymerase-Sigma 54 Reveal New and Conserved Regulatory Strategies. Science V. 349 882 2015.
ISSN: ESSN 1095-9203
PubMed: 26293966
DOI: 10.1126/SCIENCE.AAB1478