Zinc in PDB 3k0v: Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution

The structure of Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution, PDB code: 3k0v was solved by R.Mir, G.Vikram, M.Sinha, N.Singh, S.Sharma, P.Kaur, T.P.Singh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	62.00 / 1.91
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	61.810, 50.134, 65.545, 90.00, 107.10, 90.00
R / Rfree (%)	20.7 / 23.7

The structure of Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Zinc atom in the Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution (pdb code 3k0v). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution, PDB code: 3k0v:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn81 b:18.0 occ:1.00 OE1 A:GLU659 2.2 15.9 1.0 OE2 A:GLU659 2.3 17.4 1.0 CD A:GLU659 2.5 16.7 1.0 O A:GLY653 3.7 23.0 1.0 CG A:GLU659 4.0 16.7 1.0 O A:ARG654 4.5 19.7 1.0 CA A:PRO655 4.6 17.1 1.0 CB A:GLU659 4.8 16.7 1.0 N A:THR656 4.8 15.3 1.0 C A:GLY653 4.9 23.2 1.0 CG2 A:THR656 4.9 14.6 1.0

Zinc binding site 2 out of 2 in the Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Removal of Sugars and Sugars-Like Molecules From the Solution By C- Lobe of Lactoferrin: Crystal Structure of the Complex of C-Lobe with Beta-D-Glucopyranosyl-(1->4)-Beta-D-Galactopyranosyl-(1->4)-Alpha-D- Glucopyranose at 1.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn82 b:24.6 occ:1.00 O A:HOH893 2.0 28.4 1.0 NE2 A:HIS588 2.1 18.8 1.0 O A:HOH1240 2.3 28.4 1.0 CE1 A:HIS588 3.0 17.8 1.0 CD2 A:HIS588 3.1 18.4 1.0 ND1 A:HIS588 4.1 19.1 1.0 CG A:HIS588 4.2 17.4 1.0 O A:HOH807 4.6 19.0 1.0 O A:HOH894 4.7 23.8 1.0

R.Mir, R.P.Kumar, N.Singh, G.P.Vikram, M.Sinha, A.Bhushan, P.Kaur, A.Srinivasan, S.Sharma, T.P.Singh. Specific Interactions of C-Terminal Half (C-Lobe) of Lactoferrin Protein with Edible Sugars: Binding and Structural Studies with Implications on Diabetes. Int.J.Biol.Macromol. V. 47 50 2010.
ISSN: ISSN 0141-8130
PubMed: 20371371
DOI: 10.1016/J.IJBIOMAC.2010.03.021