Zinc in PDB 3gjn: Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins

The structure of Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins, PDB code: 3gjn was solved by O.Dym, D.S.Tawfik, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.94 / 2.48
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	59.650, 53.713, 79.334, 90.00, 105.59, 90.00
R / Rfree (%)	24.2 / 27.6

The binding sites of Zinc atom in the Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins (pdb code 3gjn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins, PDB code: 3gjn:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn600 b:38.2 occ:1.00 NE2 B:HIS569 1.9 31.9 1.0 NE2 B:HIS573 2.0 36.8 1.0 ND1 B:HIS544 2.3 31.4 1.0 O B:HOH601 2.5 22.8 1.0 CD2 B:HIS569 2.9 35.8 1.0 CE1 B:HIS573 2.9 38.2 1.0 CE1 B:HIS569 3.0 29.2 1.0 CE1 B:HIS544 3.1 35.0 1.0 CD2 B:HIS573 3.1 40.1 1.0 CG B:HIS544 3.3 35.1 1.0 CB B:HIS544 3.7 34.2 1.0 CA B:HIS544 3.7 34.6 1.0 ND1 B:HIS573 4.0 39.3 1.0 CG B:HIS569 4.0 34.3 1.0 ND1 B:HIS569 4.0 33.6 1.0 N B:ALA545 4.1 39.1 1.0 CG B:HIS573 4.1 41.2 1.0 NE2 B:HIS544 4.2 33.0 1.0 CD2 B:HIS544 4.3 34.4 1.0 C B:HIS544 4.4 36.6 1.0 O B:ALA545 4.6 37.4 1.0 O B:LEU543 4.7 31.7 1.0 N B:HIS544 4.8 34.2 1.0

Zinc binding site 2 out of 2 in the Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Following Evolutionary Paths to High Affinity and Selectivity Protein- Protein Interactions Using COLICIN7 and Immunity Proteins within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn600 b:44.9 occ:1.00 ND1 C:HIS544 2.1 31.3 1.0 NE2 C:HIS569 2.2 36.5 1.0 NE2 C:HIS573 2.2 43.5 1.0 O C:HOH601 2.4 15.4 1.0 CE1 C:HIS544 2.9 36.0 1.0 CD2 C:HIS569 3.0 37.3 1.0 CE1 C:HIS573 3.1 42.4 1.0 CD2 C:HIS573 3.2 47.7 1.0 CE1 C:HIS569 3.2 35.6 1.0 CG C:HIS544 3.3 36.3 1.0 CA C:HIS544 3.5 37.0 1.0 CB C:HIS544 3.7 37.4 1.0 N C:ALA545 3.8 39.1 1.0 C C:HIS544 4.1 37.8 1.0 NE2 C:HIS544 4.1 34.6 1.0 CG C:HIS569 4.2 35.9 1.0 ND1 C:HIS573 4.3 44.7 1.0 ND1 C:HIS569 4.3 35.7 1.0 CD2 C:HIS544 4.3 34.6 1.0 CG C:HIS573 4.3 47.7 1.0 O C:LEU543 4.5 33.5 1.0 O C:ALA545 4.6 36.6 1.0 N C:HIS544 4.6 36.5 1.0 C C:ALA545 4.9 38.2 1.0 CA C:ALA545 5.0 38.0 1.0

K.B.Levin, O.Dym, S.Albeck, S.Magdassi, A.H.Keeble, C.Kleanthous, D.S.Tawfik. Following Evolutionary Paths to Protein-Protein Interactions with High Affinity and Selectivity Nat.Struct.Mol.Biol. V. 16 1049 2009.
ISSN: ISSN 1545-9993
PubMed: 19749752
DOI: 10.1038/NSMB.1670