Zinc in PDB 2wwy: Structure of Human Recq-Like Helicase in Complex with A Dna Substrate

The structure of Structure of Human Recq-Like Helicase in Complex with A Dna Substrate, PDB code: 2wwy was solved by A.C.W.Pike, Y.Zhang, C.Schnecke, A.Chaikuad, T.Krojer, C.D.O.Cooper, F.Vondelft, C.H.Arrowsmith, J.Weigelt, A.Edwards, C.Bountra, O.Gileadi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.38 / 2.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	58.333, 174.218, 100.229, 90.00, 93.31, 90.00
R / Rfree (%)	19.8 / 23.6

The binding sites of Zinc atom in the Structure of Human Recq-Like Helicase in Complex with A Dna Substrate (pdb code 2wwy). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Human Recq-Like Helicase in Complex with A Dna Substrate, PDB code: 2wwy:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Human Recq-Like Helicase in Complex with A Dna Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Human Recq-Like Helicase in Complex with A Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1595 b:59.2 occ:1.00 SG A:CYS475 2.3 57.8 1.0 SG A:CYS453 2.3 57.9 1.0 SG A:CYS471 2.3 67.3 1.0 SG A:CYS478 2.3 72.3 1.0 CB A:CYS478 3.2 72.1 1.0 CB A:CYS471 3.3 70.6 1.0 CB A:CYS475 3.4 56.5 1.0 CB A:CYS453 3.6 57.3 1.0 N A:CYS475 3.9 60.4 1.0 CA A:CYS453 3.9 54.5 1.0 N A:ARG454 4.1 60.3 1.0 CA A:CYS475 4.2 57.6 1.0 CA A:CYS478 4.4 71.2 1.0 N A:CYS478 4.4 68.1 1.0 C A:CYS453 4.4 52.2 1.0 C A:MET474 4.7 60.2 1.0 CA A:CYS471 4.8 73.2 1.0 C A:CYS475 4.8 57.5 1.0 N A:MET474 4.8 65.6 1.0 O A:CYS475 4.9 59.6 1.0 N A:ARG455 5.0 56.2 1.0

Zinc binding site 2 out of 2 in the Structure of Human Recq-Like Helicase in Complex with A Dna Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Human Recq-Like Helicase in Complex with A Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1595 b:57.4 occ:1.00 SG B:CYS475 2.3 59.5 1.0 SG B:CYS471 2.3 62.7 1.0 SG B:CYS478 2.3 62.4 1.0 SG B:CYS453 2.4 61.2 1.0 CB B:CYS471 3.2 65.9 1.0 CB B:CYS478 3.2 62.1 1.0 CB B:CYS475 3.4 57.6 1.0 CB B:CYS453 3.5 61.4 1.0 CA B:CYS453 3.9 59.2 1.0 N B:CYS475 3.9 60.2 1.0 N B:ARG454 4.1 58.9 1.0 CA B:CYS475 4.2 58.1 1.0 CA B:CYS478 4.4 61.8 1.0 C B:CYS453 4.4 56.9 1.0 N B:CYS478 4.5 59.0 1.0 C B:MET474 4.7 57.0 1.0 CA B:CYS471 4.7 67.8 1.0 N B:MET474 4.8 61.6 1.0 C B:CYS475 4.9 58.4 1.0 N B:ARG455 4.9 57.2 1.0 O B:CYS475 4.9 60.3 1.0

A.C.W.Pike, Y.Zhang, C.Schnecke, A.Chaikuad, T.Krojer, C.D.O.Cooper, F.Von Delft, C.H.Arrowsmith, J.Weigelt, A.Edwards, C.Bountra, O.Gileadi. RECQ1 Helicase-Driven Dna Unwinding, Annealing, and Branch Migration: Insights From Dna Complex Structures Proc.Natl.Acad.Sci.Usa V. 112 4286 2015.
ISSN: ISSN 0027-8424
PubMed: 25831490
DOI: 10.1073/PNAS.1417594112